Abstract: The present invention discloses an immunological test for the detection and specific determination of autoantibodies against testicular antigens which are associated with inflammation-related fertility disorders of male mammals in a biological sample of a male mammal, in particular the detection of testicular ER-60 autoantibodies and/or transferrin autoantibodies. The immunological test is utilized to detect the presence of immunologically-caused and infection-related infertility in male mammals, particularly in humans.
Abstract: Testing systems and methods are disclosed for detecting a pregnancy marker of an animal. A test kit may include a first standard with a first concentration of the marker, a second standard with a second concentration of the marker lower than the first concentration, and at least three test surfaces coated with a biomolecular recognition element selected to bind with the marker. The test may also include a reagent solution with a conjugated biomolecular recognition element that binds with the marker, and a visual indicator that produces a visually detectable change when reacting with the conjugated biomolecular recognition element bound to each test surface. A detectable change generated by the marker from the sample with an intensity greater than the first concentration yields a pregnant result, lower than the second concentration yields a not pregnant result, and between the first and second concentrations yields a retest result.
Type:
Grant
Filed:
March 1, 2011
Date of Patent:
September 24, 2013
Assignee:
Bio Tracking, LLC
Inventors:
Charles W. Passavant, Joshua R. Branen, Robert Garth Sasser, Jeremy Michael Howard
Abstract: Generally, conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like, are described herein.
Type:
Grant
Filed:
June 13, 2012
Date of Patent:
August 27, 2013
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Jianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
Abstract: The invention provides immunoassays for detecting serum auto-antibodies reactive against a phospholipase A2 receptor (PLA2R) and uses thereof for diagnosis and prognosis evaluation of idiopathic membranous nephropathy (MN).
Type:
Grant
Filed:
January 14, 2011
Date of Patent:
August 13, 2013
Assignees:
Boston Medical Center Corporation, Le Centre National de la Recherche Scientifique
Inventors:
David J Salant, Laurence H. Beck, Gerard Lambeau
Abstract: Luminescence assays and compositions for assay of biomolecular interaction and activity and detection of modulators of biomolecular interaction and activity are provided. Technology described herein has utility in a variety of assay formats and types, for example, simultaneous monitoring multiple parameters which affect interaction and activity of biological molecules. Compositions and methods are provided herein which include a first solid-phase support associated with a first specific binding agent and a photosensitizer; a second solid-phase support associated with a second specific binding agent and a first emission system; and a third solid-phase support associated with a third specific binding agent and a second emission system.
Type:
Grant
Filed:
February 22, 2010
Date of Patent:
July 16, 2013
Assignee:
PerkinElmer BioSignal, Inc.
Inventors:
Philippe Roby, Roger Bosse, Mathieu Arcand
Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
Type:
Grant
Filed:
March 15, 2012
Date of Patent:
July 16, 2013
Assignee:
Intrinsic Bioprobes, Inc.
Inventors:
Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
Abstract: A method for the detection in a body fluid of perlecan polypeptide fragments that are biomarkers of tumor metastasis, and antibodies for detecting these fragments are described. An immunoassay kit for detecting the presence of these biomarkers in a body fluid, such as serum or urine, is also described.
Abstract: A method for identifying a ligand or compound which binds to albumin comprises the steps of contacting a reaction mixture comprising a site-specific probe and albumin in the presence and the absence of the ligand or compound and measuring either dissociation constant KD, inhibitor concentration IC50 or fluorescence displacement; whereby a change in KD, IC50 and/or fluorescence in the presence of the ligand or compound is indicative of the ligand or compound binds to albumin.
Type:
Grant
Filed:
September 20, 2010
Date of Patent:
July 2, 2013
Assignee:
Janssen Pharmaceutica NV
Inventors:
James K. Kranz, Gabrielle C. Todd, Matthew J. Todd
Abstract: The invention relates to lipid bilayer coated beads and methods of using those beads in delivery systems, in immunoassays, in analytical assays and the like.
Type:
Grant
Filed:
August 21, 2006
Date of Patent:
June 18, 2013
Inventors:
Gabriel P. Lopez, Reema Zeineldin, Menake E. Piyasena, Sireesha Chemburu
Abstract: A method of detecting liver damage in a subject comprises measuring the level of caspase-3 generated cytokeratin-18 fragments in the bodily sample. The level of measuring the level of caspase-3 generated cytokeratin-18 fragments is then correlated with liver disease progression.
Type:
Grant
Filed:
December 28, 2010
Date of Patent:
June 11, 2013
Assignee:
The Cleveland Clinic Foundation
Inventors:
Ariel E. Feldstein, Anna Wieckowska, Nizar Zein
Abstract: A multi-dimensional method for simultaneously analyzing multiple analytes within a sample solution, comprising adding affinity selectors to a sample solution containing analytes to be measured, the affinity selectors having an affinity for one or more of the analytes within the sample solution; allowing immune complexes to form between the affinity selectors and the analytes; partially or totally resolving the formed immune complexes from non-analyte substances within the sample solution; dissociating the resolved immune complexes; separating the analytes and the affinity selectors of the dissociated immune complexes from one another by capturing the analytes through a surface adsorption process; transferring the captured analytes to a detection means; and resolving the analytes with the detection means in accordance with their mass-to-charge ratios.
Type:
Grant
Filed:
March 10, 2010
Date of Patent:
June 4, 2013
Assignee:
Perfinity Biosciences, Inc.
Inventors:
Fred E. Regnier, Nicholas B. Herold, Kevin W. Meyer
Abstract: The present specification discloses SNAP-25 immune response inducing compositions, methods of making ?-SNAP-25 antibodies that selectively binds to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, ?-SNAP-25 antibodies that selectively bind to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, methods of detecting retargeted endopeptidase activity, and methods of detecting neutralizing ?-re-targeted endopeptidase antibodies.
Type:
Grant
Filed:
March 12, 2010
Date of Patent:
June 4, 2013
Assignee:
Allergan, Inc.
Inventors:
Joanne Wang, Hong Zhu, Dianne D. Hodges, Ester Fernandez-Salas
Abstract: Described herein is an in vitro method for the determination of the formation of endothelins in serious diseases, in particular cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient, in which relatively long-lived peptide fragments of the processed primary prepro- or proendothelins are determined which contain neither the actual biologically active endothelin nor its direct precursor big endothelin. In particular, disclosed are antibodies and kits for selectively detecting these fragments.
Abstract: A multi-stage method for diagnosing an immunologic food sensitivity or intolerance in a companion animal. Firstly a saliva or blood spot or other non-serum bodily fluid sample is collected. The screening the saliva or blood spot or other non-serum bodily fluid sample detects the presence of at least one of IgA or IgM antibody to a particular food ingredient or composition. An immunologic food sensitivity or intolerance based on the presence of the antibody is diagnosed. Secondly a blood sample is collected and serum from the sample is screened to detect the semi-quantitative or quantitative presence of at least one of an IgA, IgM or IgG antibody or immune complex to a particular food ingredient or composition. An immunologic food sensitivity or intolerance based on the presence of the antibody or immune complex is diagnosed. Thirdly, a biologically active nutrient in relation to the animal from a molecular dietary signature is determined.
Abstract: A multi-stage method for diagnosing an immunologic food sensitivity or intolerance in a companion animal. Firstly a saliva or blood spot or other non-serum bodily fluid sample is collected. The screening the saliva or blood spot or other non-serum bodily fluid sample detects the presence of at least one of IgA or IgM antibody to a particular food ingredient or composition. An immunologic food sensitivity or intolerance based on the presence of the antibody is diagnosed. Secondly a blood sample is collected and serum from the sample is screened to detect the semi-quantitative or quantitative presence of at least one of an IgA, IgM or IgG antibody or immune complex to a particular food ingredient or composition. An immunologic food sensitivity or intolerance based on the presence of the antibody or immune complex is diagnosed. Thirdly, a biologically active nutrient in relation to the animal from a molecular dietary signature is determined.
Abstract: The present invention in one embodiment uses detection of isoforms of CAP37 as an early detection marker for a chronic inflammatory-associated disease. Chronic inflammatory-related diseases may include, for example, atherosclerosis, psoriasis, and osteoarthritis. The invention is also directed to isoforms of CAP37 protein having apparent molecular weights of about 135 kDa (±15 kDa) and about 100 kDa (±15 kDa) as assessed by SDS PAGE under reducing conditions, and their use as biomarkers for the presence of a chronic inflammatory-associated disease in a subject. The invention is also directed to monoclonal and/or polyclonal antibodies raised against such isoforms of CAP37 protein and kits and diagnostic tests comprising these antibodies.
Type:
Grant
Filed:
October 14, 2010
Date of Patent:
May 28, 2013
Assignee:
The Board of Regents of the University of Oklahoma
Abstract: The invention provides a multiparametric method of assessing the reaction of a patient's immune system to a test subject. The invention compares a patient sample reacted with a test sample and a third party sample and combines the assessments of the multiple parameters to correlate the test reaction with a clinical event.
Type:
Grant
Filed:
June 5, 2006
Date of Patent:
April 23, 2013
Assignee:
University of Pittsburgh—Of the Commonwealth System of Higher Education
Abstract: A method of evaluating performance of an activation gas deactivating an antigenic substance including the steps of causing the antigenic substance and the activation gas to react with each other, to obtain a processed antigenic substance, and causing an antibody against the antigenic substance with the processed antigenic substance to measure binding activity of the processed antigenic substance with the antibody is provided, whereby an evaluation method that can accurately and easily evaluate performance of an activation gas deactivating an antigenic substance is provided.
Abstract: A method of detecting liver damage in a subject comprises measuring the level of caspase-3 generated cytokeratin-18 fragments in the bodily sample. The level of measuring the level of caspase-3 generated cytokeratin-18 fragments is then correlated with liver disease progression.
Type:
Grant
Filed:
December 28, 2010
Date of Patent:
April 2, 2013
Assignee:
The Cleveland Clinic Foundation
Inventors:
Ariel E. Feldstein, Anna Wieckowska, Nizar Zein
Abstract: The invention relates to methods for the quantitative or qualitative detection of an analyte in an assay and adequate reagents therefor, particularly a homogeneous binding test. According to the invention, an analyte-specific binding partner R1 comprises more than one specific binding point for a specific binding partner X that is associated with a component of a signal-forming system while a second analyte-specific binding partner R2 comprises more than one specific binding point for a specific binding partner Y which is also associated with a component of a signal-forming system.