Abstract: The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods described herein, DNA binding protein of a cell is linked (e.g., covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein of binds.

Abstract: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

Abstract: Regulatory nucleotide sequences for a novel Pichia pastoris gene, designated PpSEC10 gene, and the nucleotide sequences and respective amino acid sequences for the secretion leader and the mature Sec10p protein components of the precursor polypeptide encoded by this novel gene are provided. These compositions are useful in methods for expression and secretion of proteins when assembled in proper reading frame, individually or in combination, within a DNA construct that further comprises a nucleotide sequence encoding a protein of interest. Vectors comprising the DNA constructs of the invention can be used to transform a yeast host cell, which can then be cultured to obtain the secreted protein of interest. Kits useful in this method and in methods of detection of the Sec10p protein using antibodies are also disclosed.

Abstract: Long term calorie restriction has the benefit of increasing life span. Methods to screen interventions that mimic the effects of calorie restriction are disclosed. Extensive analysis of genes for which expression is statistically different between control and calorie restricted animals has demonstrated that specific genes are preferentially expressed during calorie restriction. Screening for interventions which produce the same expression profile will provide interventions that increase life span. In a further aspect, it has been discovered that test animals on a calorie restricted diet for a relatively short time have a similar gene expression profile to test animals which have been on a long term calorie restricted diet.

Abstract: The invention relates to methods and products for analyzing polymers. The polymers are analyzed by reconstructing sequence information from population data sets. The data sets include information about polymer dependent impulses arising from the polymers. The invention is also a method for linearly analyzing polymers by assessing the intensity of a signal arising from the polymer. The signal is generated as units and/or units specific markers pass a fixed station. The quantitative intensity of the signal is proportional to the number of units and/or unit specific markers giving rise to the signal.

Abstract: Methods of reducing blood pressure levels in hypertensive subjects through the identification of subjects with an allele and/or genotype at a gene locus that positively correlates with improved success in reducing blood pressure levels, as compared to other alleles and/or genotypes at the same gene locus, and through the engagement of these subjects in exercise training for a period of time sufficient for the reduction of the subjects' blood pressure levels.

Abstract: There is used at least one probe array obtained by arraying particles having various probes, respectively, fixed thereon (probe particles) in a definite order in a holder. A plurality of capillaries or grooves packed with various kinds, respectively, of probe particles are arrayed in parallel, and one of particles contained in each capillary or groove is injected into another capillary or groove to produce a probe array in which the various kinds of probe particles are arrayed in a constant and definite order. Various fluorophore-labeled DNA's are measured at the same time by attaching various probes to particles, respectively, of different sizes. A probe array composed of various fixed DNA probes can easily be produced, and there can be provided a probe array for detecting various DNA's which is composed of various fixed arbitrary DNA probes.

Abstract: An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.

Abstract: A method of diagnosing a disease associated with a genetic polymorphism in a TNF&bgr; gene comprises determining the genotype of said TNF&bgr; gene in an animal. The method can be used to diagnose CAP or diagnose predisposition or susceptibility to CAP. Compositions for said diagnosis are provided. Methods of treatment of CAP or predisposition or susceptibility to CAP are provided, comprising identifying an individual having CAP a predisposition or susceptibility to the disease and subsequently treating that individual in accordance with treatments for such conditions as are known to those of skill in the art. Also provided are methods of identifying and diagnosing a predisposition for septic shock, along with methods of treatment.

Abstract: An educational kit comprised of novel a novel polypeptide, alpha helical protein-34 (Zalpha34), polynucleotides that encode Zalpha34 and antibodies to Zalpha34. The present invention also relates to polynucleotide and polypeptide molecules for Zalpha34. The polypeptides, and polynucleotides encoding them, are hormonal and may be used to promote spermatogenesis. The present invention also includes antibodies to the Zalpha34 polypeptides, which can be used to inhibit spermatogenesis.

Abstract: Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby the second component is internalized within the biological cell. At least one of the first and second components has a fluorescent label. The amount of binding between the first and second components within the cell is determined by measuring a level of polarized and/or depolarized fluorescence emitted from within the biological cell.

Abstract: The present invention provides novel identification polypeptides containing multiple copies of an antigenic domain joined in tandem to provide increased sensitivity for the detection and purification of target peptides, a cleavable linking sequence and optionally a spacer domain. Further provided are hybrid polypeptide molecules composed of an identification polypeptide and a target peptide which are produced by recombinant DNA technology and purified using affinity chromatography using one or more ligands. Accordingly, also provided are DNA expression vectors containing DNA encoding for identification polypeptides and methods for using such identification polypeptides for the purification of target peptides. Also provided are methods of constructing DNA vectors encoding the novel identification polypeptides and DNA expression vectors encoding the identification polypeptides linked to a target peptide.

Abstract: Disclosed is a method for diagnosing the tumorigenic grade of a malignant tissue. The method entails determining the amount of dystroglycan protein of the malignant tissue relative to a standard. Suitable methods for determining the amount of dystroglycan protein of the tissue are provided, and include measuring the amount of mRNA transcripts which encode dystroglycan, and also performing western blot analysis or immunofluorescence analysis on the tissue components to detect &agr;-dystroglycan or &bgr;-dystroglycan. An antibody probe which binds specifically to the C-terminus of &bgr;-dystroglycan, is provided. This method is applicable to human malignant tissue, especially adenocarcinoma, and preferably prostate or mammary adenocarcinoma. This method can also be applied to the detection of a cancerous disease state in a tissue of a patient, with a decreased level of dystroglycan protein being indicative of the presence of cancer.

Abstract: The invention relates to DNA sequences from fimbrial gene clusters of Escherichia coli strain DSM 6601. This strain has two chromosomal fimbrial gene clusters, namely type I (fim) and FIC (foc) gene clusters. DNA sequences of the main subunits fimA and focA are represented by SEQ ID NOS: 1 and 2.

Abstract: The present invention provides methods of correlating genetic variants in the beta-chemokine receptor 5 (CCR5) locus, more specifically CCR5 promoter alleles and genotypes, with HIV-1 transmission and/or disease progression in individuals and populations.

Abstract: Disclosed are nucleic acid and amino acid sequences encoded by a novel, prostate specific gene (UC41) and diagnostic techniques for the detection of human prostate cancer utilizing such nucleic acid and amino acid sequences. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate cancer are described. Methods of treatment for prostate cancer utilizing antisense constructs or antibodies specific for UC41 gene products are also described.

Abstract: The present invention includes a process for extracting a stereoisomer fro biomass. The method comprises providing biomass and subjecting the biomass to substantially instantaneous pressurization and depressurization to separate cellulose, hemicellulose, and lignin from the biomass. The hemicellulose is hydrolyzed to form hemicellulose hydrolysates. The hydrolysates are separated using chromatography.

Abstract: The present invention provides methods and kits for determining the identity of a nucleotide at a variant site in a target nucleic acid of interest, including, for example, point mutations and single nucleotide polymorphisms. The methods involve conducting template-dependent extension reactions in the presence of a mixture of nucleotides that include at least one labeled extendible nucleotide and at least one labeled non-extendible nucleotide that are selected to be complementary to the nucleotides that potentially occupy the variant site. The particular labeled nucleotide incorporated into the extension products is characteristic of the nucleotide at the variant site.

Abstract: Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers.

Abstract: Methods are presented for determining the presence of an inversion in the factor VIII gene which cause hemophilia A. The methods encompass long distance, multiplex PCR (including overlapping PCR). The use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO aid in successfully performing the PCR. The use of a novel technique called subcycling PCR can also be applied as part of the methods. The technique allows for the determination of whether a person is homozygous or hemizygous for the inversion and has hemophilia A or whether a person is heterozygous for the inversion and is a carrier. The technique of long distance, multiplex PCR including use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO are applicable to the determination of the presence of other gross chromosomal aberrations such as deletions/inversions, translocations and inversions.