Abstract: Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.

Abstract: The present invention provides a process for the cell fusion of strains of Phaffia rhodozyma thereby providing novel strains of Phaffia rhodozyma.

Abstract: The invention relates to novel DNA and amino sequences for a human MK protein. Also described are expression vectors and host cells useful in a method for production of the MK protein.

Abstract: A transformation system is provided for C. tropicalis, comprising constructs and microorganisms, as well as methods for preparing constructs and microorganisms, and transforming microorganisms. Particularly, a yeast transformation system comprising auxotrophic hosts which are auxotrophic in either an amino acid, purine or pyrimidine pathways and employ DNA constructs comprising genes encoding biosynthetic enzymes which functionally complement the auxotrophies to prototrophies.

Abstract: The present invention provides a method for transforming an actinomycete with an integrating vector which has the advantages of high transformation rates into a broad host range, site-specific integration, and stable maintenance without antibiotic selection. Also provided are methods for the increased production of antibiotics and for the production of hybrid antibiotics.

Abstract: A purified and isolated cryIII-type gene was obtained from a novel B.t. strain. The gene has a nucleotide base sequence coding for the amino acid sequence illustrated in FIG. 1. The 74.4 kDa protein produced by this gene is an irregularly shaped crystal that is toxic to coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica.

Abstract: A glucose-repressible gene comprising amino acid coding sequences of a desired protein and also an upstream regulatory region. An efficient expression vector comprising the regulatory region may be constructed to direct in a controlled manner, the production of recombinant products in N. crassa and related organisms.

Abstract: Disclosed are respiratory-deficient yeast except Saccharomyces cerevisiae AH22R.sup.-, said respiratory-deficient yeast being transformed with a DNA containing a gene encoding a protein foreign to yeast and a method for preparing a protein foreign to yeast described above, which comprises culturing the yeast, and producing and accumulating the protein in a culture. The respiratory-deficient yeast of the present invention can produce a higher amount of protein than its parent strain.

Abstract: The present invention provides nucleotide sequences which are selectively expressed in pre-B cells, probes comprising a polynucleotide hybridizing specifically to such a nucleotide sequence and methods for the production of such probes. These probes may be used for identifying pre-B cells. The invention further provides polypeptides translated from a transcript comprising a nucleotide sequence which is selectively expressed in pre-B cells or parts thereof, antibodies against these polypeptides and methods for the preparation and use of the polypeptides and antibodies raised against them.

Abstract: Insect control agents comprising aThis invention was made with funding from the National Institutes of Health (Grant No. AI-23719) and with Hatch Act funding from the U.S. Department of Agriculture (Grant No. GE000918). The U.S. government may have certain rights in this invention.

Abstract: Novel Bacillus thuringiensis genes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-active B. thuringiensis microbes. The DNA encoding the B. thuringiensis toxins can be used to transform various prokaryotic and eukaryotic microbes to express the B. thuringiensis toxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

Abstract: Novel serine protease inhibitors and genes encoding the same are described.