Abstract: An inline filter housing with a biodegradable, hydrophilic material that operates in conjunction with a field sampling apparatus to both concentrate field sampled environmental DNA particles from water samples and to automatically preserve the captured DNA via desiccation, thus avoiding filter membrane transfer steps, chemicals or cold storage preservation requirements. The hydrophilic filter housing is capable of rapidly preserving the field sampled environmental DNA captured on the filter membrane at ambient field temperatures.

Abstract: The disclosure relates to an arrangement (100?) in a capillary driven fluidic system for preventing wicking of a fluid along an edge (105) between a first substrate (110?) and a second substrate (120?), said arrangement comprising: a first substrate (110?) including a microfluidic channel (114) arranged to house the fluid, a second substrate (120?) arranged to cover a portion of the first substrate (110?), wherein the microfluidic channel (134) of the first substrate (110?) meets the second structure (120?) at a position (124) along an edge (105) defined between the first (110?) and second (120?) substrates, wherein the first and the second substrate collectively define a trench (130) for stopping wicking of the fluid along the edge (105), said trench (130) being arranged in at least one of the first substrate (110?) and the second substrate (120?), and located at a distance from said position (124) along the edge (105) and intersecting the edge (105).

Abstract: In a first transfer station, a second transfer station, and a third transfer station, a plurality of sample containers are transferred while being stored from a transfer source rack to a transfer destination rack row. In this case, for each of the sample containers to be a transfer target, a specific transfer destination rack is selected from the transfer destination rack row based on a content of processing to be applied in the future to a sample within the sample container.

Abstract: A device includes an input chamber, an attractant chamber, a migration channel arranged in fluid communication between an outlet of the input chamber and inlet of the attractant chamber, a baffle arranged in fluid communication between the outlet of the input chamber and the migration channel or within the migration channel, and an exit channel in fluid communication with the migration channel at a point beyond the baffle and before the migration channel enters the inlet of the attractant chamber. The baffle is configured to inhibit movement of a first type of cell through the baffle to a greater extent than the baffle inhibits movement of a second type of cell through the baffle.

Abstract: An apparatus and method for isolating bacterial particles in a sample using a container with material in temporary fluid blocking position to lower orifice in the container, a separation medium having an electrical conductivity lower than and physical density greater than that of the sample above the material that supports a sample concentrate after passing through the separation medium when exposed to centrifugal force, a heating element for liquefying the material to permit flow into a chamber past an electrode array that attracts and holds subject particles. The system allows rapid detection and isolation of particles from samples from animal, human, environmental sites, a bio-industrial reactor or a food or beverage production facility requiring relatively small volumes, short incubation times resulting in structurally intact particles for further analysis. Testing may be completed in a single unit that requires decreased technician manipulation, fewer steps and a decrease in cross-contamination.

Abstract: Systems and methods for sealing a plurality of reservoirs of a microchip element with a sealing grid are provided. For example, in one embodiment, a microchip element comprises a primary substrate having a plurality of reservoirs defined therein. The microchip element also includes a single continuous sealing groove defined in the primary substrate that extends around each of the plurality of reservoirs. In addition, the microchip element includes a sealing substrate comprising a single continuous sealing protrusion extending therefrom. The single continuous sealing protrusion corresponds to and is configured to mate with the single continuous sealing groove to form a hermetic bond between the primary substrate and the sealing substrate. In this manner, the single continuous sealing groove and the single continuous sealing protrusion form a sealing grid about the plurality of reservoirs.

Abstract: A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows.

Abstract: A well plate assembly with an interior channel system in the well plate lid provides a more efficient and uniform distribution of fluid into a receptacle positioned below the well plate lid. The channel system in the lid allows air, or other fluid, to pass through with the use of a pump to each of a plurality of channels in the receptacle. Inlet and outlet valves in the lid prevent a gasket positioned between the lid and the receptacle from releasing contact with the receptacle due to high pressure experienced during the injection of fluid into the assembly. Specifically, pressure under a specified tolerance passes through the inlet valves, and if the pressure within the channel system exceeds the limit of the outlet valve, the outlet valve opens and allows air to escape the lid safely without disturbing the fluid flow into the channels below.

Abstract: An electroporation device is disclosed, which includes a first tubular electrode including a first inlet and a first outlet; an aqueous solution supply connected to the first tubular electrode and configured for supplying an aqueous solution to the first tubular electrode and for allowing a droplet of the aqueous solution to be discharged from the first outlet of the first tubular electrode, the aqueous solution contains a cell and a delivery target substance; a second tubular electrode including a second inlet and a second outlet, wherein the second inlet is spaced away from the first outlet at a spacing corresponding to a size of a portion of the droplet; a power supply for applying a voltage to the electrodes for electroporation of the cell in the droplet; and a droplet sucking unit connected to the second electrode and configured for allowing the droplet to be sucked into the second electrode.

Abstract: A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

Abstract: The present invention generally relates to systems and techniques for manipulating fluids and/or making droplets. In certain aspects, the present invention generally relates to droplet production. The droplets may be formed from fluids from different sources. In one set of embodiments, the present invention is directed to a microfluidic device comprising a plurality of droplet-making units, and/or other fluidic units, which may be substantially identical in some cases. Substantially each of the fluidic units may be in fluidic communication with a different source of a first fluid and a common source of a second fluid, in certain embodiments. In one aspect, substantially the same pressure may be applied to substantially all of the different sources of fluid, which may be used to cause fluid to move from the different sources into the microfluidic device. In some cases, the fluids may interact within the fluidic units, e.g., by reacting, or for the production of droplets within the microfluidic device.

Abstract: A method for providing a solution of a substance in a microfluidic device includes providing a dispersion of a first medium and of a lyophilizate of the substance, the lyophilizate being insoluble in the first medium, and adding a second medium to the dispersion, the lyophilizate being soluble in the second medium. The method further includes dissolving the lyophilizate in the second medium such that the solution of the substance in the second medium is obtained, and separating the solution obtained by the dissolving of the lyophilizate from the first medium.

Abstract: A microfluidic injection and manifold assembly includes a microfluidic chip having at least a first fluid port and a second fluid port, and a fluid pathway between the first fluid port and the second fluid port. A manifold has a seat on which the microfluidic chip is received, and at least a first fluid channel. The fluid channel has an external fluid port spaced from the seat and an internal fluid port in the seat and connected in fluid communication with the first fluid port of the microfluidic chip. At least a first injector is secured to the manifold and has a plunger and a drive assembly. The drive assembly is activatable to force the plunger into the external fluid port of the manifold to force fluid from the first fluid channel of the manifold into the fluid pathway of the microfluidic chip.

Abstract: A method and filtering system are described. The filtering system comprises a vacuuming plate having a base with a plurality of outlet openings extending therethrough, a plurality of walls extending from the base to define a cavity, and a vacuum port in fluid communication with the cavity; and a filtering unit mounted to the vacuuming plate and enclosing the cavity. The filtering unit comprises a filter plate with a plurality of filter openings extending therethrough and aligned with the outlet openings; a filtering membrane extending across the filter plate and covering the plurality of filter openings; and a piercing plate on the filter plate maintaining the filtering membrane therebetween, the piercing plate having a plurality of vessel piercing members extending away from the filtering membrane, the vessel piercing members having conduits extending through the piercing plate, the conduits aligned with the filter openings to allow fluid flow therebetween.

Abstract: Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen-specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

Abstract: The present invention discloses a flow path structure of a cartridge for nucleic acid extraction comprising: a plurality of elongated grooves formed on the surface of the cartridge body; and a shaft hole formed in the center of the body, wherein the plurality of grooves have a starting point formed around the shaft hole and an ending point extending from the starting point toward the outer region of the body.

Abstract: The present invention provides a membrane carrier 3 comprising a flow path 2 and a detection zone 3y, wherein a microstructure is provided at the bottom of the flow path 2 and a mean surface roughness of the microstructure is 0.005 to 10.0 ?m.

Abstract: Methods and systems for optofluidic device fabrication and design are herein disclosed. In examples, a user may manufacture the optofluidic device using stereolithography (SLA) three-dimensional (3D) printing, in which photosensitive resin is exposed to a focused laser, solidifying specific areas of resin. The optofluidic device may guide light for a broad wavelength range from a liquid or gas channel comprised within and may be used to guide the emission of light and particles of interest to a detector to identify the particles.

Abstract: A sensor cassette insertable into an analysis module for carrying out electrochemical measurement processes in sample fluids, including a sensor carrier which is essentially planar and carries electrochemical sensor elements for determining chemical and/or physical parameters of the sample fluids. The sensor elements are connected to conductor tracks formed on the sensor carrier. A cover part is arranged on the sensor carrier, in which at least one groove-shaped measuring channel that is intended for the flow of the sample fluid and opens towards the sensor carrier is formed, which measuring channel is connected to at least one electrochemical sensor element and is fluidly connected to at least one fluid connector arranged on the side facing away from the sensor carrier. The cover part is a two-component injection molded part having a hard component and a soft, component. The measuring channel is formed in the soft component over its entire length.

Abstract: The present invention aims at providing a fluidic device that can hold a large amount of solutions in a reservoir without depending on an attitude. The reservoir includes a meandering flow path including: a plurality of first flow paths that extend linearly along a first direction and that are arranged to be spaced in a second direction crossing the first direction; and a second flow path that extends linearly along the second direction such that a connection between first end sides of the adjacent first flow paths and a connection between second end sides of the adjacent first flow paths are alternately switched along the second direction for each first flow path, wherein the meandering flow path meanders along the second direction.