Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
Abstract: The present invention relates to organic compounds, usable as ligands in the preparation of lanthanide complexes or of certain water-soluble transition metals, a method for preparation thereof, and the use of said organic compounds as fluorescent probes.
Type:
Grant
Filed:
September 20, 2012
Date of Patent:
May 3, 2016
Assignee:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Inventors:
Raymond Ziessel, Mathieu Starck, Alexandra Sutter
Abstract: This invention relates to the engineering of animal cells, preferably mammalian, more preferably rat, that are deficient due to the disruption of tumor suppressor gene(s) or gene product(s). In another aspect, the invention relates to genetically modified rats, as well as the descendants and ancestors of such animals, which are animal models of human cancer and methods of their use.
Type:
Grant
Filed:
July 1, 2010
Date of Patent:
April 19, 2016
Assignee:
Transposagen Biopharmaceuticals, Inc.
Inventors:
Eric M. Ostertag, John Stuart Crawford, Joseph Ruiz
Abstract: The invention relates to a method for obtaining a variety of recombinant bacteriophages in which the screening proteins have been modified by the insertion in their genetic sequence of randomly produced oligonucleotides, and to bacteriophages banks that can be obtained according to said method.
Abstract: A mutant bacterial acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine is described. Also described is a method for producing branched-chain L-amino acids using a bacterium from the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by the use of the acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine. This acetolactate synthase contains a mutant small subunit encoded by the mutant ilvN gene.
Abstract: The invention relates to a method for identifying in a sample molecules with the capacity to bind to the different members of a molecule library, wherein the molecules which are in the sample are fractionated based on a physicochemical property of the sample and subsequently transferred from the support in which said molecules have been separated to a second support in which the different members of the molecule library are grouped into microarrays such that they uniformly coat the surface of the support. This technique is especially interesting for the identification in a sample of glycoproteins with affinity for a lectin library as well as for the identification of compounds capable of modulating the glycosylation of proteins and for the rapid characterization of alterations in the glycosylation pattern of a sample of proteins, which can be useful in the diagnosis of diseases in which there are alterations in cell glycosylation.
Type:
Grant
Filed:
June 22, 2011
Date of Patent:
February 9, 2016
Assignee:
Centro de Investigación Cooperativa en Biomateriales (CIC biomaGUNE)
Inventors:
Niels-Christian Reichardt, Juan Etxebarria Ruiz
Abstract: The present system provides novel methods and compositions for selecting a particular strand of RNA and/or producing a cDNA library that results in an unbiased representation of RNA in a sample.
Abstract: The present invention relates to a method for enhancing the health of an animal comprising administering to the animal a composition comprising Bacillus subtilus QST 713 or a mutant thereof.
Type:
Grant
Filed:
August 24, 2012
Date of Patent:
February 2, 2016
Assignee:
Bayer CropScience LP
Inventors:
Joseph Earl Schmidt, Desmond Rito Jimenez
Abstract: This application discloses a light-responsive hydrogel-based platform that can modulate multiple microenvironmental signals to direct the differentiation of human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) into neuronal cells. The invention provides novel methods for directing differentiation of neural stem cells into neurons useful for treatment of degenerative diseases or disorders, including but not limited to Alzheimer's, Parkinson's, or spinal cord injury (SCI).
Type:
Grant
Filed:
December 8, 2011
Date of Patent:
January 12, 2016
Assignee:
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
Abstract: A tissue handling apparatus is provided comprising a support and a locator, for processing and embedding tissue samples. The support and cover form a chamber, within which a tissue sample is oriented prior to processing. After processing, the tissue sample within the chamber is embedded and the locator is removed, leaving the tissue sample correctly oriented in embedding material, mounted to a support, ready for microtoming.
Type:
Grant
Filed:
September 6, 2006
Date of Patent:
January 12, 2016
Assignee:
LEICA BIOSYSTEMS MELBOURNE PTY LTD
Inventors:
Eduardo Vom, Chester John Henderson, Neville John Farmer
Abstract: A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide derived from human 28S rRNA into the human-derived cells. The method for preparing neoplastically transformed cells from human-derived cells can be utilized when a variety of human normal cells are induced to be neoplastically transformed in order to elucidate cancer onset mechanisms, so that the method can be effectively utilized in search of target molecules for a new medicament.
Abstract: Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.
Type:
Grant
Filed:
August 6, 2008
Date of Patent:
December 1, 2015
Assignee:
Anthrogenesis Corporation
Inventors:
Mohammad A. Heidaran, Xiaokui Zhang, Lin Kang, Andrew Zeitlin, Vanessa R. Voskinarian-Berse, Stewart Ernest Abbott
Abstract: Use of low dose streptozocin in the preparation of an animal model for screening drugs for treatment of antoimmune type 1 diabetes is disclosed, in which streptozocin is administrated intravenously at a dose of 15-30 mg/kg per time for 5 days and administrated again on the 7th day and 14th day after last time of offering drug. A method for preparing rhesus monkey model of autoimmune type 1 diabetes and autoimmune type 1 diabetes animal model obtained are also disclosed.
Type:
Grant
Filed:
March 26, 2010
Date of Patent:
November 17, 2015
Assignee:
West China Hospital, Sichuan University
Inventors:
Yanrong Lu, Jingqiu Cheng, Shaoping Deng, Younan Chen, Bole Tian, Gang Mai, Yan Ren, Li Wang, Hongxia Li, Lingling Wei, Xi Jin, Chaofeng Qiao, Wensheng Zhang, Sirong He, Li Zeng
Abstract: The present invention provides transgenic non-human animals and non-human mammalian somatic and germ cells harboring a human DNA sequence encoding Alzheimer's Disease (AD) derived tau protein, capable of inducing AD pathology in transgenic animals. Alzheimer's tau protein is expressed on specific genetic backgrounds allowing also simulation of different human diseases including hypertension, diabetes, hyper-cholesterolemia, which are associated with neurodegeneration and are considerable risk factors for AD development. Transgenic animals and cells of the invention exhibit neurofibrillary pathology and may serve as in vivo and in vitro assay systems for screening and developing therapeutic and preventive substances and also diagnostic markers and probes for tauopathies and AD.
Abstract: A method of producing a compound originating from a polysaccharide-based biomass includes at least one of a saccharification step that produces a sugar solution containing a monosaccharide and/or an oligosaccharide from a product obtainable by hydrolyzing the polysaccharide-based biomass; a fermentation step that ferments the sugar solution containing the monosaccharide and/or oligosaccharide originating from the polysaccharide-based biomass; and a treatment that removes a fermentation inhibitor with the use of a separation membrane having a glucose removal rate and an isopropyl alcohol removal rate which simultaneously satisfy the following relationships (I) and (II) when a 500 ppm aqueous glucose solution at pH 6.5 at 25° C. and a 500 ppm aqueous isopropyl alcohol solution at pH 6.5 at 25° C. are respectively permeated through the membrane at an operation pressure of 0.
Abstract: The invention relates to methods of proliferating stem cells. More particularly, the invention relates to the use of glycosaminoglycans or proteoglycans to promote the growth of stem cells in ex vivo culture, while preserving their multipotentiality.
Type:
Grant
Filed:
December 5, 2008
Date of Patent:
September 29, 2015
Assignee:
Agency for Science, Technology and Research
Abstract: Described are medical grafting materials that include a base substrate material and an adherent fibrous mass of extracellular matrix components received upon the substrate material. Also described are methods of preparing and using such materials.
Abstract: Methods for treating inflammation are disclosed, such as for treating ocular inflammation. In some embodiments, the ocular inflammation is inflammation of an ocular surface, such as keratitis. The methods include administering to a subject with inflammation a therapeutically effective amount of SLURP1, or a nucleic acid encoding SLURP1, thereby treating the inflammation.
Type:
Grant
Filed:
November 4, 2013
Date of Patent:
September 15, 2015
Assignee:
University of Pittsburgh—Of the Commonwealth System of Higher Education
Inventors:
Shivalingappa Kottur Swamynathan, Sudha Swamynathan, Kristine-Ann Gallegos Buela, Robert Lee Hendricks
Abstract: The invention relates to a multiwell system, characterized that said multiwell system having at least 3 wells, wherein said wells have a volume between 0.125 and 4.0 mm3.
Type:
Grant
Filed:
July 6, 2011
Date of Patent:
September 15, 2015
Assignee:
UNIVERSITEIT TWENTE
Inventors:
Gustavo Andres Higuera, Lorenzo Moroni, Clemens Antoni van Blitterswijk