Patents Examined by Mark Shibuya
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Patent number: 8329423Abstract: The present invention provides liquid crystal-based devices and methods for bioagent detection. In certain aspects, the present invention is directed to devices and methods utilizing liquid crystals and membranes containing polymerized targets that can report the presence of bioagents including, but not limited to, enzymes, antibodies, and toxins.Type: GrantFiled: January 21, 2011Date of Patent: December 11, 2012Assignee: Wisconsin Alumni Research FoundationInventors: Nicholas L. Abbott, Joon-Seo Park, Sarah Teren, David J. Beebe, Eric A. Johnson
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Patent number: 8318093Abstract: Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity.Type: GrantFiled: May 17, 2007Date of Patent: November 27, 2012Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Shan X. Wang, Robert L. White, Chris D. Webb, Guanxiong Li
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Patent number: 8309368Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: June 11, 2007Date of Patent: November 13, 2012Assignee: BioArray Solutions, Ltd.Inventor: Michael Seul
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Patent number: 8309367Abstract: The present invention concerns a novel means by which liquids can be moved or mixed. Microwaves strike and heat materials that are highly susceptible to microwave heating. The susceptible materials are on, within, or near materials that melt or change shape in response to temperature increases. Upon microwave irradiation, these materials change shape (e.g., shrink or melt), causing the movement of liquids. The invention is important in many microfluidics applications, especially in biomedical analysis, where it is valuable to be able to move small volumes of liquids (e.g., on a microarray chip).Type: GrantFiled: April 14, 2005Date of Patent: November 13, 2012Assignee: Mirari Biosciences, Inc.Inventors: Richard Saul, Mark T. Martin
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Patent number: 8304257Abstract: Improved methods for preparing thiolate passivated gold nanoparticles are provided resulting in a narrower size distribution. By alternating oxidation and reduction of the particles, after oxidation, the thiolates may be readily displaced with a different thiol, followed by reduction to prevent further reaction. Proteins can be rigidly bound to the gold nanoparticles by binding an scFv for a target protein to the gold nanoparticles, where the scFv prior or subsequent to binding to the gold nanoparticles may be complexed with the target protein. The protein is rigidly held and its structure readily determined using electron microscopy.Type: GrantFiled: March 8, 2007Date of Patent: November 6, 2012Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Christopher J. Ackerson, Pablo Jadzinsky, Roger D. Kornberg
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Patent number: 8298485Abstract: Contemplated herein is an automated microscope slide antigen recovery and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments for individually and independently processing a plurality of individual microscope slides. The apparatus preferably features independently movable slide support elements each having an individually heatable heating plate. Each slide support element preferably supports a single microscope slide. Each microscope slide can be enclosed within an individual pressurizable reaction compartment. Pressures exceeding 1 atm or below 1 atm can be created and maintained in the reaction compartment prior to, during or after heating of the slide begins.Type: GrantFiled: May 24, 2006Date of Patent: October 30, 2012Inventors: Lee Angros, Thomas Lee Byers
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Patent number: 8293492Abstract: Novel conjugates and immunogens derived from lenalidomide and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of thalidomide and lenalidomide in biological fluids.Type: GrantFiled: November 22, 2011Date of Patent: October 23, 2012Assignee: Saladax Biomedical Inc.Inventors: Salvatore J Salamone, Jodi Blake Courtney, Alexander Volkov, Howard Sard, Vishnumurthy Hegde
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Patent number: 8283183Abstract: A detection device and a detecting method using the detection device are provided in which a magnetic particle is used as a marker particle, and the ratio of a region with reversed magnetization to the whole area of a free layer of a magnetoresistive effect film is increased by a stray magnetic field generated through a biochemical reaction from the magnetic particle remaining on a surface of the magnetoresistive effect film, so that a large detection signal is obtained and obtained detection data can be stored with stability.Type: GrantFiled: April 26, 2005Date of Patent: October 9, 2012Assignee: Canon Kabushiki KaishaInventors: Takashi Ikeda, Norihiko Utsunomiya, Junta Yamamichi
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Patent number: 8216788Abstract: The present disclosure relates to methods and compositions for the detection of infectious proteins or prions in samples, including the diagnosis of prion related diseases. One embodiment is an ultrasensitive method for detecting PrP-res (PrPSc) that allows the use of recombinant PrP-sen (rPrP-sen) as a substrate for seeded polymerization. A sample is mixed with purified rPrP-sen to make a reaction mix which is incubated to permit aggregation of the rPrP-sen with the PrP-res that may be present in the sample. Any aggregates are intermittently disaggregated by agitation (for example by sonication) and the reaction allowed to proceed to amplify target substrate. Any rPrP-res(Sc) in the reaction mix is detected to indicate the presence of PrP-res in the original sample. This assay, which is called rPrP-PMCA, is surprisingly much faster than existing PMCA methods, yet it still retains sufficient sensitivity to detect extremely low levels of PrP-res.Type: GrantFiled: July 21, 2008Date of Patent: July 10, 2012Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Byron W. Caughey, Ryuichiro Atarashi, Roger A. Moore
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Patent number: 8198100Abstract: A detection device and a detecting method using the detection device are provided in which a magnetic particle is used as a marker particle, and the ratio of a region with reversed magnetization to the whole area of a free layer of a magnetoresistive effect film is increased by a stray magnetic field generated through a biochemical reaction from the magnetic particle remaining on a surface of the magnetoresistive effect film, so that a large detection signal is obtained and obtained detection data can be stored with stability.Type: GrantFiled: April 26, 2005Date of Patent: June 12, 2012Assignee: Canon Kabushiki KaishaInventors: Takashi Ikeda, Norihiko Utsunomiya, Junta Yamamichi
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Patent number: 8193003Abstract: Accurate measurement cannot be performed due to magnetic signals from magnetic impurities included in a specimen container. By finding a difference between two measurement signals obtained by applying an external magnetic field for orientation to respective normal and reverse directions, the magnetic signals from the magnetic impurities included in the specimen container which are not dependent on the external magnetic field for orientation can be cancelled. The influence of the magnetic impurities included in the specimen container is reduced, and the signal of the intended bound magnetic marker can be measured with high sensitivity.Type: GrantFiled: May 12, 2008Date of Patent: June 5, 2012Assignee: Hitachi, Ltd.Inventors: Akira Tsukamoto, Daisuke Suzuki, Akihiko Kandori
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Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Patent number: 8178359Abstract: A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.Type: GrantFiled: September 28, 2001Date of Patent: May 15, 2012Assignee: Bayer Healthcare AGInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 8173372Abstract: The detection of endothelial cell antibodies has been proven clinically important for successful organ transplantation. Disclosed are methods of isolating Tie-2+ and CD34? precursor endothelial cells for use in donor-specific crossmatching.Type: GrantFiled: May 16, 2003Date of Patent: May 8, 2012Assignee: AbSorber ABInventor: Suchitra Sumitran-Holgersson
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Patent number: 8158365Abstract: An antibody that reacts with 2-O-desulfated acharan sulfate, a hybridoma that produces the antibody, a detection method and a detection kit to which the antibody is applied are disclosed. The antibody that reacts with 2-O-desulfated acharan sulfate can be produced by immunizing a mammal using as an antigen a substance obtained by chemically bonding a protein to 2-O-desulfated acharan sulfate.Type: GrantFiled: April 27, 2007Date of Patent: April 17, 2012Assignee: Seikagaku CorporationInventors: Kiyoshi Suzuki, Takeshi Ishimaru, Koji Yamamoto, Yeong Shik Kim
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Patent number: 8153081Abstract: According to one embodiment of the present invention, an optical-based test sensor for use in the determination of an analyte in a liquid sample is disclosed. The test sensor includes a base, a polymer carrier, and a test membrane. The base has a capillary channel formed in a surface of the base that is adapted to move a liquid sample from an inlet to a reaction area formed in the base. The polymer carrier has a lower surface adhered to the surface of the base and is disposed over at least a portion of the capillary channel. The test membrane, which contains a reagent, is adhered to the lower surface of the polymer carrier and extends from the polymer carrier into the reaction area.Type: GrantFiled: May 14, 2004Date of Patent: April 10, 2012Assignee: Bayer Healthcare LLCInventor: Rex J. Kuriger
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Patent number: 8153436Abstract: Methods of assaying for disease-associated crystal species in biological samples are described. Such methods involve contacting a patient sample with an excess of a detectable crystal-tagging compound reactive with a plurality of crystal species under conditions that allow the detectable crystal-tagging compound to react with a plurality of crystal species, if present, to form tagged crystal species complexes. Substantially all unreacted tagging compound is then removed. If desired, chemical, enzymatic, or physical treatment can be used to selectively degrade some, but not, of the tagged crystal species. Assessment of soluble versus crystal-associated label is then performed.Type: GrantFiled: February 14, 2008Date of Patent: April 10, 2012Assignees: The United States of America as represented by the Department of Veterans Affairs, University of MiamiInventor: Herman S. Cheung
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Patent number: 8148171Abstract: A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. A secondary reagent, such as a metal or magnetic particle, is added to the beadset to assist in the analysis. Detection techniques, such as such as light scatter, Rayleigh scatter, Raman scatter, surface plasmon resonance, magnetic induction, or magnetoresistance are used to detect the particle labels.Type: GrantFiled: December 31, 2004Date of Patent: April 3, 2012Assignee: Luminex CorporationInventors: Don J. Chandler, Van S. Chandler
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Patent number: 8133680Abstract: The present invention provides methods, devices and kits for detecting a ligand. The methods involve capturing a ligand from a sample with an affinity substrate that includes a receptor for a ligand, transferring captured ligand to a detection surface and detecting the ligand on the detection surface with a liquid crystal. Accordingly, the capture step is decoupled from the detection step.Type: GrantFiled: September 23, 2004Date of Patent: March 13, 2012Assignee: Wisconsin Alumni Research FoundationInventors: Nicholas L. Abbott, Matthew L. Tingey, Brian H. Clare, Chang-Hyun Jang
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Patent number: 8114683Abstract: Detection of magnetic beads at temperature below room temperature can increase the signal level significantly as compared to the same detection when performed at room temperature. Additional improvement is obtained if the beads are below 30 nm in size and if deviations of bead size from the median are small. A preferred format for the beads is a suspension of super-paramagnetic particles in a non-magnetic medium.Type: GrantFiled: March 5, 2007Date of Patent: February 14, 2012Assignee: Headway Technologies, Inc.Inventor: Yuchen Zhou
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Patent number: 8092742Abstract: An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.Type: GrantFiled: November 23, 2009Date of Patent: January 10, 2012Inventor: Lee Angros