Patents Examined by Mary E. Ceperley
  • Metal chelate containing compositions for use in chemiluminescent assays
    Patent number: 6916667
    Abstract: Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
    Type: Grant
    Filed: December 9, 2003
    Date of Patent: July 12, 2005
    Assignee: Dade Behring Marburg GmbH
    Inventors: Sharat Singh, Edwin F. Ullman
  • Immunological test kit comprising an immunologically invisible peg copolymer conjugated to one or more immunologically reactive substances
    Patent number: 6913936
    Abstract: A novel compound comprising an immunologically invisible polyethylene glycol copolymer is used to carry one or more immunologically reactive substances. The novel compounds may be used as part of kits for immunological assays.
    Type: Grant
    Filed: October 17, 2001
    Date of Patent: July 5, 2005
    Assignee: University of Medicine and Dentistry of New Jersey
    Inventors: Bo Qiu, Guobao Zhang, Stanley Stein, Leonard H Sigal, Michael Brunner, Michael Katz
  • Preservative solutions
    Patent number: 6905886
    Abstract: This invention relates to preservative solutions for peptides, for example, parathyroid hormone, insulin-like growth factor binding protein 3 (IGFBP3), adrenocorticotropic hormone (ACTH) and mixtures thereof. Preferably, the peptides preserved by the solutions are non-reconstituted. In one embodiment, the preservative solutions comprise a polyvinyl alcohol, EDTA component and molybdic component.
    Type: Grant
    Filed: March 13, 2002
    Date of Patent: June 14, 2005
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Lata Sundaram, Ann Hoang, Stan Shimizu, Elizabeth R. Umelo-Njaka, Darcy A. Thomas
  • Particles for immunoassays and methods for treating the same
    Patent number: 6890765
    Abstract: A method of treating particles to be used in immunoassays reduces interference in particle agglutination assays. For particles having covalently bound antibodies and residual NHS-ester or sNHS-ester groups on the surface, the reactive esters are treated with an aqueous mixture containing an amine compound of formula (I): H2N—R—X??(I). The moiety —X is —NH2, —OH, or —CO2CH2CH3; and R is an alkyl group or an alkyl ether group. When —X is —NH2 or —CO2CH2CH3, R contains from 1 to 20 carbon atoms; and when —X is —OH, R contains from 4 to 20 carbon atoms.
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: May 10, 2005
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Christopher C. Lawrence, Wei Yuan, Armen B. Shanafelt
  • Process and intermediates
    Patent number: 6884874
    Abstract: Processes for preparing N-methyl-L-alanine derivatives and novel intermediates of the process. The derivatives and intermediates are useful for preparing cell-binding agent/maytansinoid complexes.
    Type: Grant
    Filed: March 17, 2003
    Date of Patent: April 26, 2005
    Assignee: SmithKline Beecham Corporation
    Inventors: Ann M. Eldridge, Johann Hiebl
  • Synthetic bioepitope compounds which can be used as standards in the biological assays of Troponin I
    Patent number: 6867011
    Abstract: The invention relates to synthetic biepitope compounds which can be used as standards in immunoassays, for the assay of troponin I, method for preparing them, compositions and kits containing such compounds as well as the immunoassay methods using such compounds.
    Type: Grant
    Filed: December 4, 1997
    Date of Patent: March 15, 2005
    Assignee: Bio-Rad Pasteur
    Inventors: Fabienne Noelle Babin, Charles Didier Calzolari, Odile Suzanne Helene Flecheux, Claude Granier, Catherine Christiane Marie Larue, Bernard Christian Pau, Francois Yves Rieunier, Sylvie Marie-France Trinquier
  • Phthalamide-lanthanide complexes for use as luminescent markers
    Patent number: 6864103
    Abstract: The present invention provides luminescent lanthanide metal chelates comprising a metal ion of the lanthanide series and a complexing agent comprising at least one phthalamidyl moiety. Also provided are probes incorporating the phthalamidyl ligands of the invention and methods utilizing the ligands of the invention and probes comprising the ligands of the invention.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: March 8, 2005
    Assignee: The Regents of the University of California
    Inventors: Kenneth N. Raymond, Stephane Petoud, Seth M. Cohen, Jide Xu
  • Benzothiazole dioxetanes
    Patent number: 6852548
    Abstract: Chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature, represented by the formulas I or II: wherein T is:
    Type: Grant
    Filed: September 5, 2001
    Date of Patent: February 8, 2005
  • Functionalized silicon surfaces
    Patent number: 6846681
    Abstract: The present invention is directed to a silicon substrate having a monolayer formed by an electrochemically-induced reaction between silicon hydride moieties on the silicon surface and optionally substituted alkynes covalently bound to the surface of the silicon substrate and to a method for electrochemically producing such a functionalized silicon substrate. The method of forming a covalently bound monolayer on a silicon surface comprises the steps of contacting the silicon surface with a C2-C24 alkyne and electrografting optionally substituted alkynes to the silicon surface.
    Type: Grant
    Filed: July 19, 2002
    Date of Patent: January 25, 2005
    Assignee: Purdue Research Foundation
    Inventors: Jillian M. Buriak, Michael P. Stewart, Edward Robins
  • Radioligand and binding assay
    Patent number: 6838291
    Abstract: The present invention is directed to the radioligand compound, [35S]-radiolabeled (+)-N-[1?-(6-cyano-1,2,3,4-tetrahydro-2(R)-naphthalenyl)-3,4-dihydro-4(R)-hydoxyspiro[2H-1-benzopyran-2,4?-piperidin]-6-yl]methanesulfonamide. Also within the scope of this invention is a method for identifying compounds that bind to the IKr channel, and may have antiarrhythmic activity.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: January 4, 2005
    Assignee: Merck & Co., Inc.
    Inventors: John W. Butcher, David A. Claremon, Thomas M. Connolly, Jerzy Karczewski, Kenneth S. Koblan, Matthew J. Kostura, Nigel J. Liverton, Dennis C. Dean, David G. Melillo
  • Polymer brushes for immobilizing molecules to a surface and having water-soluble or water-dispersible segments therein and probes bonded thereto
    Patent number: 6833276
    Abstract: Sensors for determining the presence and concentration of bio-molecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface that is modified with a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, and in particular the use of an iniferter initiator, which allows for controlled architecture polymers to modify the surface of the substrate. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.
    Type: Grant
    Filed: July 24, 2001
    Date of Patent: December 21, 2004
    Assignee: Symyx Technologies, Inc.
    Inventors: Gerrit Klaerner, Ralph B. Nielsen, Paul Mansky, Didier Benoit, Dominique Charmot, Bernd Jandeleit, Laura T. Mazzola
  • Carbopyronine fluorescent dyes
    Patent number: 6828159
    Abstract: The invention relates to the use of carbopyronine compounds of general formula (I) as marker groups in methods for detecting analytes. The invention also relates to novel carbopyronine compounds and to a method for producing same.
    Type: Grant
    Filed: October 26, 2001
    Date of Patent: December 7, 2004
    Inventors: Karl-Heinz Drexhage, Jutta Arden-Jacob, Jörg Frantzeskos, Alexander Zilles
  • Phosphoprotein binding agents and methods of their use
    Patent number: 6818454
    Abstract: The invention provides reagents and methods for characterizing (i.e., identification and/or quantitation) the phosphorylation states of proteins. Proteins may be post-transcriptionally modified such that they contain phosphate groups at either some or all of their serine, threonine, tyrosine, histidine, and/or lysine amino acid residues. In many cases the extent to which a protein is phosphorylated determines it bioactivity, i.e., its ability to effect cell functions such as differentiation, division, and metabolism. Hence, a powerful tool for diagnosing various diseases and for furthering the understanding of protein—protein interactions is provided.
    Type: Grant
    Filed: February 16, 2001
    Date of Patent: November 16, 2004
    Assignee: Battelle Memorial Institute
    Inventors: Michael B. Goshe, Thomas P. Conrads, Timothy D. Veenstra, Ellen A. Panisko
  • Passivation of porous semiconductors
    Patent number: 6815162
    Abstract: A method is disclosed for stabilizing porous silicon. A porous silicon structure having a surface terminated with hydrogen atoms is subjected to organic thermal processing to substitute the hydrogen atoms with a protective organic layer. The resulting structure are found to have unprecedented stability.
    Type: Grant
    Filed: March 12, 2001
    Date of Patent: November 9, 2004
    Assignee: National Research Council of Canada
    Inventors: Rabah Boukherroub, Danial D. M. Wayner, David J. Lockwood, Sylvie Morin
  • Pharmaceutical uses and synthesis of diketopiperazines
    Patent number: 6815214
    Abstract: The synthesis of novel diketopiperazines, their use in inhibiting cellular events such as those involving NFK-&agr;, NFK-&bgr; and in the treatment of inflammation events, a combinatorial library of diverse diketopiperazines and process for their synthesis as a library and as individual compounds. In particular novel diketopiperazines are disclosed including their synthesis and use in cellular events such as activation of the transcription factor, nuclear factor, TNF-&agr;, TNF-&bgr; and also apoptosis.
    Type: Grant
    Filed: December 28, 2001
    Date of Patent: November 9, 2004
    Assignee: Celltech R & D, Inc.
    Inventors: Jim P. Boyce, J. Jeffry Howbert, John C. Tabone
  • ECL labels having improved non-specific binding properties, methods of using and kits containing the same
    Patent number: 6808939
    Abstract: Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non-specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: October 26, 2004
    Assignees: IGEN International, Inc., BioVeris Corporation
    Inventors: George B. Sigal, Howie Tjiong, Liwen Dong, Md. Athar Masood, Richard C. Titmas
  • Simple method for labeled conjugate production
    Patent number: 6809186
    Abstract: The present invention relates to methods for coupling labels to particular target moieties. The coupling reactions of the present invention use temporal spacing of the reactants through phase change (i.e. by rapid freezing) to control the initiation and termination of reaction. This process results in a simplified and improved method for linking labels to specific binding moieties using N-hydroxysuccinimide chemistry. The present invention further relates to kits comprising all necessary components to easily and rapidly make protein conjugates.
    Type: Grant
    Filed: December 6, 2001
    Date of Patent: October 26, 2004
    Assignee: Martek Biosciences Corporation
    Inventors: John P. Morseman, Xiangfei Zeng
  • Immunoassay for LSD and 2-oxo-3-hydroxy-LSD
    Patent number: 6794496
    Abstract: The present invention provides hapten derivatives useful for the preparation of antigens, antibodies and reagents for use in immunoassays for the detection of LSD and 2-oxo-3-hydroxy LSD. In the present invention, the 2-oxy LSD nucleus is derivatized out of the indole nitrogen to form an aminoalkyl derivative. The resulting haptens can then be further modified at this functionalized position for linking to appropriate immunogenic or labeling groups to provide reagents for immunoassays having substantially equal specificity for both LSD and 2-oxo-3-hydroxy-LSD.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: September 21, 2004
    Assignee: Roche Diagnostics Corporation
    Inventors: Mitali Ghoshal, Stephen Vitone, Gerald F. Sigler, Alan J. McNally
  • Tertiary amine compounds for use in immunoassays
    Patent number: 6777246
    Abstract: A reagent for use in immunoassays reduces interference in particle agglutination assays. The reagent contains particles having covalently bound antibodies and a tertiary amine compound of formula (I): N(R1—X)(R2—Y)(R3—Z)  (I). The moieties R1, R2, and R3 are independently alkyl or alkyl ether. The moieties X, Y, and Z are independently —OH, —O—R4, —S—R4, —C(═O)—OH, —C(═O)—OR4, or —C(═O)—NHR4 (R4 is alkyl).
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: August 17, 2004
    Assignee: Roche Diagnostics GmbH
    Inventors: Christopher C. Lawrence, Armen B. Shanafelt
  • Nitroso compounds and their use as spin traps
    Patent number: 6767715
    Abstract: A compound of the formula wherein X is Cl or CH3, or a salt thereof, is useful as a spin trap in various assays.
    Type: Grant
    Filed: December 11, 2001
    Date of Patent: July 27, 2004
    Assignee: Randox Laboratories Ltd.
    Inventors: Lynne Hamilton, Paul Graham Winyard