Patents Examined by Michael Paul Shimek
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Patent number: 11385207Abstract: The present disclosure provides a method for analyzing an additive in a water-based polymer sample, comprising the steps of: (S1) putting the water-based polymer sample containing a polymer, the additive, and water as a solvent into a vial; (S2) putting a porous pouch containing a superabsorbent polymer (SAP) into the vial to absorb the water into the superabsorbent polymer; (S3) removing the porous pouch from the vial and collecting the concentrated polymer sample remaining in the vial; and (S4) performing a pyrolysis gas chromatography (Py-GC)/mass spectrometer (MS) analysis by introducing the concentrated polymer sample to the Py-GC/MS.Type: GrantFiled: September 9, 2020Date of Patent: July 12, 2022Assignee: LG CHEM, LTD.Inventors: Mi Kyoung Jin, Rin Jang, So Yeon Kim, Byoung Hyoun Kim, Bumgyu Choi
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Patent number: 11385243Abstract: A stool resuspension solution comprising a hemoglobin stabilization reagent is provided. In some embodiments, the hemoglobin stabilization reagent may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. A method of stabilizing hemoglobin in a stool sample in the solution is also provided, as well as a sample collection device containing the solution.Type: GrantFiled: March 15, 2019Date of Patent: July 12, 2022Assignee: EXACT SCIENCES CORPORATIONInventors: Keith D. Fourrier, Jacquelyn T. Hennek, Michael J. Domanico, William G. Weisburg, Graham P. Lidgard, Kathleen S. Harings, Daniel J. Simpson
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Patent number: 11371990Abstract: The disclosed embodiments concern methods, devices, and systems for identifying candidate biomarkers useful for the diagnosis, prognosis, monitoring and screening and/or as targets for the treatment of diseases and conditions in subjects, in particular autoimmune and infectious diseases. The identification of candidate biomarkers is predicated on identifying discriminating peptides present on a peptide array, which can distinguish samples from different subjects having different health conditions by the binding patterns of antibodies present in the samples.Type: GrantFiled: November 10, 2017Date of Patent: June 28, 2022Assignee: COWPER SCIENCES INC.Inventors: Robert William Gerwien, Theodore Michael Tarasow, Jonathan Scott Melnick, Kathryn Frances Sykes, Michael William Rowe
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Patent number: 11369896Abstract: The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when run alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.Type: GrantFiled: October 21, 2020Date of Patent: June 28, 2022Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Dingjiang Liu, Lin Luo, Long Xu
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Patent number: 11365214Abstract: The invention relates to a method for the treatment of body fluid proteins, by which proteins from body fluids such as blood or urine are extracted by adding a certain proportion of high molecular polymer solution under low temperature condition followed by denaturation and reduction by adding a certain concentration of surfactant and tris(2-carboxyethyl) phosphine (TCEP) under a high temperature condition. Subsequently, the iodoacetic acid brushes grafted on silica microspheres called as solid-phase alkylation reagents are added into protein solution, which can react rapidly with the protein sulfhydryl group. After centrifugation, the microspheres are obtained and repeatedly washed with methanol and buffer to remove interferences such as sugars, salts, surfactants, lipids to obtain high-purity proteins, and finally protease is added to digest proteins into peptides. After centrifugation, the peptide products are obtained, and directly analyzed by liquid chromatography-mass spectrometry (LC-MS) system.Type: GrantFiled: July 12, 2017Date of Patent: June 21, 2022Assignee: DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACADEMY OF SCIENCESInventors: Lihua Zhang, Huiming Yuan, Zhigang Sui, Kaiguang Yang, Yukui Zhang
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Patent number: 11360080Abstract: A method for identifying an AhR-phospho-ROR?t protein complex inhibitor, comprising: (a) providing a cell culture, in which cells in the culture express AhR protein and phospho-ROR?t protein; (b) incubating the cell culture in the presence of a test agent; (c) assaying the level of the AhR-phospho-ROR?t protein complex in the presence of the test agent; (d) comparing the level of the AhR-phospho-ROR?t protein complex in the presence of the test agent with a control; and (e) identifying the test agent as the inhibitor of the AhR-phospho-ROR?t protein complex when the comparing step indicates that there is a reduction in the level of the AhR-phospho-ROR?t protein complex in the presence of the test agent as compared with the control. A method for identifying a GLK?IQGAP1 protein complex inhibitor is also disclosed. Use of identified inhibitors in the manufacture of a medicament for treating a disease is also disclosed.Type: GrantFiled: August 15, 2019Date of Patent: June 14, 2022Assignee: NATIONAL HEALTH RESEARCH INSTITUTESInventors: Tse-Hua Tan, Huai-Chia Chuang
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Patent number: 11360063Abstract: A method for analyzing an antioxidant content contained in a semiconductive material for a cable, which includes an amine-based antioxidant. The method can provide an accurate quantitative analysis value obtained by a comparison with the actual amount used, through gas chromatography (GC)/a flame ionization detector (FID).Type: GrantFiled: July 23, 2018Date of Patent: June 14, 2022Inventors: Moon Ja Nam, Byoung Hyoun Kim, Min Jeong Lee
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Patent number: 11353466Abstract: The present invention relates to methods and compositions for quantifying hemoglobin using, inter alia, a peroxidase substrate and hydrogen peroxide.Type: GrantFiled: January 15, 2019Date of Patent: June 7, 2022Assignee: CELLICS THERAPEUTICS, INC.Inventor: Weidong Xu
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Patent number: 11345970Abstract: Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.Type: GrantFiled: August 19, 2021Date of Patent: May 31, 2022Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Eric Hunt, Gregory Patton, Guoping Ren, Zhiru Li, Andrew Barry, Nicole Nichols, Catherine B. Poole, Harriet M. Strimpel, Ivan R. Correa, Jr., Clotilde Carlow, Esta Slayton
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Patent number: 11346842Abstract: The invention, in part, includes methods of single molecule protein sequencing that include using weak binding spectra in the amino acid identification.Type: GrantFiled: June 22, 2020Date of Patent: May 31, 2022Assignee: Massachusetts Institute of TechnologyInventors: Edward Boyden, Adam Henry Marblestone, Samuel Gordon Rodriques
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Patent number: 11330830Abstract: Described herein are kits for assessing the calcium oxalate titration test of an animal as well as methods for predicting the risk of calcium oxalate stone formation. Further described herein are methods and compositions for—inter alia—lowering the specific gravity of urine and lowering the calcium oxalate titration test in felines. In particular, diets and methods utilizing certain amounts and ratios of arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are disclosed herein.Type: GrantFiled: December 8, 2017Date of Patent: May 17, 2022Assignee: Hills Pet Nutrition, Inc.Inventors: Dale Scherl, Dennis Jewell, Jeffrey Brockman, Stephen Davidson, Christina Golder, Albert Avila
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Patent number: 11327059Abstract: The present invention relates to a dianhydride analysis method. The method can stably analyze a dianhydride which has high reactivity and low solubility. Furthermore, the method can minimize the problem that a reaction product disturbs an analysis result, thereby improving accuracy of the analysis result.Type: GrantFiled: October 4, 2019Date of Patent: May 10, 2022Inventors: So Yeon Kim, Joon Seok Lee, Bumgyu Choi, Byoung Hyoun Kim
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Patent number: 11327075Abstract: It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.Type: GrantFiled: August 21, 2017Date of Patent: May 10, 2022Assignee: The Regents of the University of CaliforniaInventors: Daniel Takashi Kamei, Benjamin Ming Wu, David Yuan Pereira, Chloe Michelle Wu, Matthew Foosing Yee, Joshua Anthony Keefe, Christina Caroline Pearce, Amir Adam Dailamy, Vincent K. Wong, Nguyen Khoi Le
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Patent number: 11320438Abstract: Tryptophan degradation is a key metabolic pathway controlling immune reactions and evidence suggests that during cancer progression generation of tryptophan metabolites may be fundamental for immune escape promoting the malignant phenotype of cancer cells in an autocrine fashion. The present invention relates to methods of measuring mass tag labelled tryptophan and metabolites thereof and methods using the labelled molecules for monitoring in a subject the effectiveness of a treatment and of disease recurrence after treatment, for stratifying patients and for diagnosing suppression of an immune response in a subject.Type: GrantFiled: October 31, 2016Date of Patent: May 3, 2022Assignees: ELECTROPHORETICS LIMITED, RUPRECHT-KARLS-UNIVERSITAT, DKFZ DEUTSCHES KREBSFORSCHUNGSZENTRUMInventors: Sasa Koncarevic, Karsten Kuhn, Peter Schulz-Knappe, Ian Hugo Pike, Christiane Opitz, Michael Platten
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Patent number: 11307182Abstract: A system for determining isotopic composition of a gaseous sample. The system includes at least one gas chromatograph for separating the gaseous sample into gaseous components. Furthermore, the system includes a combustion furnace operatively coupled with the at least one gas chromatograph for oxidizing the gaseous components. Moreover, the system includes a water separator operatively coupled with the combustion furnace. Furthermore, the system includes an isotope-ratio mass spectrometer operatively coupled with the water separator. Moreover, the isotope-ratio mass spectrometer comprises an ion source for generating ion beams associated with each of the oxidized gaseous components and a mass analyser for receiving the generated ion beams from the ion source, wherein the mass analyser is operable to determine isotopic concentrations associated with each of the ion beams.Type: GrantFiled: December 6, 2018Date of Patent: April 19, 2022Assignee: COMPACT SCIENCE SYSTEMS LTDInventors: Dennis Leigh, James Leigh, Paul Jones
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Patent number: 11291998Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.Type: GrantFiled: February 2, 2021Date of Patent: April 5, 2022Assignees: Illumina, Inc., Illumina Cambridge LimitedInventors: Wesley A. Cox-Muranami, Darren Robert Segale, Oliver Jon Miller, Jennifer Olivia Foley, Tarun Kumar Khurana, Paul Crivelli, Gary Watts
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Patent number: 11287401Abstract: Disclosed is a sample pretreatment method of microextraction tube injection, comprising providing a capillary micro-extraction tube with extracting medium in it as an injector, passing a sample through the capillary micro-extraction tube, during which an analyte is extracted into an extracting medium inside the capillary micro-extraction tube; then, filling the capillary micro-extraction tube with an organic solvent and keeping the filling for a certain period of time, so that the extracted analyte is dissolved in the organic solvent inside the capillary micro-extraction tube to form an injection solution; finally, keeping one end of the capillary micro-extraction tube sealed and inserting the other end directly into an injection port of a gas chromatography, such that the injection solution is automatically ejected out from the capillary micro-extraction tube into the injection port.Type: GrantFiled: October 17, 2019Date of Patent: March 29, 2022Assignee: WUHAN SPENRICH TECHNOLOGIES CO., LTDInventors: Jun Xing, Xiaoyu Ye, Wenyu Huang
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Patent number: 11280778Abstract: A chemical oxygen demand (COD) of a sample including water is determined. At least two test specimens are obtained. Each of the test specimens include a mixture of the sample and a standard solution including potassium hydrogen phthalate (KHP) in a known concentration. Each of the specimens include a same amount of the sample. Each of the specimens include different amounts of the standard solution. Each of the specimens is diluted with water. A COD of each of the specimens is measured. A COD of the sample is determined based on the measured CODs of the specimens.Type: GrantFiled: July 27, 2020Date of Patent: March 22, 2022Assignee: Saudi Arabian Oil CompanyInventors: Nasir Ullattumpoyil, Abdulaziz M. Rueshed
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Patent number: 11280799Abstract: The invention relates to the detection of non-metabolized vitamin D. In a particular aspect, the invention relates to methods for detecting underivatized non-metabolized vitamin D by mass spectrometry.Type: GrantFiled: August 24, 2020Date of Patent: March 22, 2022Assignee: Quest Diagnostics Investments IncorporatedInventors: Brett Holmquist, Nigel Clarke
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Patent number: 11275093Abstract: The present invention relates to a method of diagnosing pulmonary hypertension (PH) in a patient, a method of monitoring the course of pulmonary hypertension in a patient, a method of determining the severity of pulmonary hypertension in a patient, and a method of differentiating between pulmonary hypertension and at least one condition selected from a group consisting of a disease associated with a risk of developing pulmonary hypertension and metabolic syndrome. In addition, the present invention relates to a kit comprising means for carrying out the above methods.Type: GrantFiled: March 8, 2017Date of Patent: March 15, 2022Assignees: CBMED GMBH CENTER FOR BIOMARKER RESEARCH IN MEDICINE, JOANNEUM RESEARCH FORSCHUNGSGESELLSCHAFT MBH, LBI-LVR-LUDWIG BOLTZMANN INSTITUTE LUNG VASCULAR RESEARCH OF THE LUDWIG BOLTZMANN GESELLSCHAFT GMBH, MEDIZIN MEDIZINISCHE UNIVERSITAT GRAZInventors: Natalie Bordag, Christoph Magnes, Sophie Narath, Edgar Gander, Andrea Olschewski, Bence M Nagy, Horst Olschewski