Patents Examined by Miquel H. Esccllon
  • Reverse transcription with Thermus thermophilus polymerase
    Patent number: 5407800
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
    Type: Grant
    Filed: June 17, 1993
    Date of Patent: April 18, 1995
    Assignee: Hoffmann-La Roche Inc.
    Inventors: David H. Gelfand, Thomas W. Myers