Patents Examined by Nelson Yang
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Patent number: 8597715Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: April 2, 2010Date of Patent: December 3, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 8460744Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: GrantFiled: February 25, 2010Date of Patent: June 11, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Patent number: 8404450Abstract: The present invention relates to an optical imaging system communicatively connected to a microwave energy producing source wherein the combination provides for increases in chemical reaction times and the ability to monitor the reactions in real time with sufficient resolution to view the location of intracellular components labeled with luminescent molecules as well as interaction with other biomolecules and responses to localized environmental variables in living cells and tissues during the application of a microwave field.Type: GrantFiled: June 4, 2008Date of Patent: March 26, 2013Assignee: University of Maryland, Baltimore CountyInventors: Chris D. Geddes, Michael J. R. Previte
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Patent number: 8349605Abstract: A waveguide sensor capable of direct, real-time detection and monitoring of analytes in the vicinity of the waveguide surface without requiring the tagging or labeling of the analyte, is described. Analytic and numerical calculations have predicted that by locally detecting either changes in the evanescent field or changes in the light coupled out of the waveguide as a result of the presence of the analyte, high detection sensitivity will be able to be achieved.Type: GrantFiled: April 12, 2006Date of Patent: January 8, 2013Assignee: Colorado State University Research FoundationInventors: Kevin L. Lear, David S. Dandy, Matthew D. Stephens, Guangwei Yuan
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Patent number: 8334140Abstract: A sensor comprises respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a cationic species, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose. For example a holographic sensor comprises a glucose-binding boronate and a cationic species held within the sensor.Type: GrantFiled: November 8, 2006Date of Patent: December 18, 2012Assignees: Smart Holograms Limited, Cambridge Enterprise LimitedInventors: Satyamoorthy Kabilan, Mei-Ching Lee, Adrian Martin Horgan, Kathryn Elizabeth Sorrell Medlock, Christopher Robin Lowe, Jeffrey Blyth
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Patent number: 8227255Abstract: A biosensor capable of analyzing an object, such as antigen, antibody, DNA or RNA, through detection of magnetic field to thereby allow washout of unbound label molecules to be unnecessary, which biosensor is compact and available at low price, excelling in detection precision. Coils are arranged at an upper part and a lower part of a magnetic sensor using a hall element as a magnetic field detection element. An object and magnetic particles having an antibody capable of specific bonding with the object bound to the surface thereof are introduced in the magnetic sensor having a molecular receptor capable of specific bonding with the object attached to the surface thereof. Therefore, a change in magnetic field by magnetic particles bonded through the molecular receptor to the surface of the magnetic sensor is detected by means of the hall element.Type: GrantFiled: January 26, 2009Date of Patent: July 24, 2012Assignee: Asahi Kasei Kabushiki KaishaInventor: Hirofumi Fukumoto
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Patent number: 7998694Abstract: The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps: incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane; exciting the measurement medium at the excitation wavelength of the energy-donating member; and measuring the FRET signal or the variations in said signal emitted in said culture medium.Type: GrantFiled: August 29, 2006Date of Patent: August 16, 2011Assignee: CIS BIO InternationalInventors: Eric Trinquet, Hervé Ansanay, Gérard Mathis
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Patent number: 7977072Abstract: Disclosed is an improved sandwich immunoassay for identifying partial proANP peptides in cardiac and sepsis diagnosis by using two antibodies which specifically bond to partial sequences in the mid-regional area of NT-proANP, extending from amino acid 53 to amino acid 83 of NT-proANP.Type: GrantFiled: October 16, 2003Date of Patent: July 12, 2011Assignee: B.R.A.H.M.S GmbHInventors: Andreas Bergmann, Joachim Struck
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Patent number: 7955836Abstract: Disclosed herein is a device comprising a pair of bellows pumps configured for efficient mixing at a microfluidic scale. By moving a fluid sample and particles in suspension through an aperture between the paired bellows pump mixing chambers, molecular collisions leading to binding between the particles and ligands in the sample are enhanced. Such devices provide an alternative for mixing that does not use a vent and can be used with a variety of particles in suspension such as magnetic beads to capture or purify useful cells and molecules.Type: GrantFiled: January 11, 2010Date of Patent: June 7, 2011Assignee: Micronics, Inc.Inventors: John Clemmens, C. Frederick Battrell, John Gerdes, Denise Maxine Hoekstra
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Patent number: 7927651Abstract: There is provided a biosensor capable of increasing a detecting sensitivity of a target substance of glutamate, by using a nano wire having excellent electrical characteristics and by immobilizing a receptor of glutamate to be detected on a substrate which is disposed between a nano wire and another nano wire and a method for manufacturing the same. The biosensor for detecting glutamate according to the present invention can be manufactured with an arrangement in which the nano wire is selectively arranged on a solid substrate in a matrix. Since this biosensor can prevent the degradation of the nano wire in the electrical characteristic, it can sensitively detect glutamate even through a small amount thereof is contained in a food so that it can be effectively used in detecting the food additive existing in the processed foodstuffs.Type: GrantFiled: April 4, 2007Date of Patent: April 19, 2011Assignees: Seoul National University Industry Foundation, Mitech CorporationInventors: Seung-Hun Hong, Byung-Yang Lee, Dong-Joon Lee
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Patent number: 7910356Abstract: A planar array having plurality of biological recognition molecules including at least two types of biological recognition molecules distributed about a substrate is disclosed. A first type of biological recognition molecules is distributed according to a first frequency and a second type of biological recognition molecules is distributed according to a second frequency. Another planar array having a plurality of biological recognition molecules including at least two kinds of biological recognition molecules is disclosed. The recognition molecules are distributed about a substrate with first kind of biological recognition molecules distributed at a first height or depth relative to a surface of the substrate and a second kind of biological recognition molecules distributed at a second height or depth relative to the surface.Type: GrantFiled: February 1, 2006Date of Patent: March 22, 2011Assignee: Purdue Research FoundationInventors: David D. Nolte, Manoj Varma, Fred E. Regnier, Leilei Peng
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Patent number: 7879560Abstract: Nanotubes and nanotube array structures comprise (a) a nanotube having an inner wall portion; and (b) a bilayer coating formed on the inner wall portions, with the bilayer coating comprised of surfactants. A secondary compound such as a protein, peptide or nucleic acid may be associated with the bilayer coating. The structures are useful for, among other things, affinity purification, catalysis, and as biochips.Type: GrantFiled: December 8, 2008Date of Patent: February 1, 2011Assignee: North Carolina State UniversityInventor: Alex I. Smirnov
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Patent number: 7842344Abstract: The present invention relates to the use of direct-write lithographic printing of proteins and peptides onto surfaces. In particular, the present invention relates to methods for creating protein and peptide arrays and compositions derived therefrom. Nanoscopic tips can be used to deposit the peptide or protein onto the surface to produce a pattern. The pattern can be dots or lines having dot diameter and line width of less than 1,000 nm. The tips and the substrate surfaces can be adapted for the peptide and protein lithography.Type: GrantFiled: March 1, 2004Date of Patent: November 30, 2010Assignee: Northwestern UniversityInventors: Chad A. Mirkin, Jung-Hyurk Lim, David S. Ginger, Jwa-Min Nam, Ki-Bum Lee, Linette Demers, Albena Ivanisevic
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Patent number: 7829328Abstract: Hollow particles for use in various types of assay devices are provided. Due to their hollow or voided structure, the particles may exhibit a variety of beneficial properties. For instance, hollow particles are generally lightweight, and thus, relatively inexpensive in comparison to other types of particles. Hollow particles may also form a stable system without requiring refrigeration or rotation. In addition, hollow particles may possess enhanced light diffraction capabilities, which may be particularly beneficial in certain types of assay devices, e.g., diffraction-based assay devices.Type: GrantFiled: May 25, 2006Date of Patent: November 9, 2010Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Kaiyuan Yang, Ning Wei, Rosann Kaylor, Chibueze Obinna Chidebelu-Eze, Zdravko Savov Atanassov
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Patent number: 7820427Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.Type: GrantFiled: September 12, 2006Date of Patent: October 26, 2010Assignee: Fluidigm CorporationInventors: Marc Unger, Ian D. Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, Geoffrey Facer
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Patent number: 7776559Abstract: A disposable blood test device comprises a substrate configured for carrying a chemical reagent and circuitry formed on the substrate. The circuitry comprises a sensor portion associated with the chemical reagent to enable measurement of at least one of a presence and a concentration of a blood analyte, and an information storage portion configured to store information indicative of a property of the chemical reagent.Type: GrantFiled: January 22, 2004Date of Patent: August 17, 2010Assignee: Hewlett-Packard Development Company, L.P.Inventors: Winthrop D. Childers, David Tyvoll
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Patent number: 7754497Abstract: This invention provides a method for immobilizing proteins comprising: step 1 of purifying target proteins to be immobilized, which have a first tag portion and a second tag portion, with the use of the first tag portion; step 2 of activating reactive groups capable of covalently binding to the proteins on a carrier for immobilization; and step 3 of allowing a solution containing the proteins purified in step 1 to react with the carrier after step 2, wherein, in step 3, the proteins are immobilized on the carrier via interactions between the second tag portion and the site of the carrier to which the second tag portion binds and via covalent binding between the reactive groups and the proteins. This method enables the stable immobilization of various types of target proteins on a carrier regardless of the amounts of target proteins and without nonspecific immobilization of contaminating proteins.Type: GrantFiled: August 24, 2004Date of Patent: July 13, 2010Assignee: Reverse Proteomics Research Institute Co., Ltd.Inventors: Tsuyoshi Yamada, Yorimasa Suwa, Takeshi Tsutsumi
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Patent number: 7723056Abstract: Lipid vesicle particles capable of being targeted to a cell type of interest, said particle incorporating a peptide which is responsive to a predetermined metabolic signal from the targeted cell so as to modulate the permeability of the particle, said particle further incorporating a species to be targeted to the cell which is activated on said modulation of permeability. The particles may be used in methods for detecting cells, methods of treating cells and also therapeutically.Type: GrantFiled: October 14, 1998Date of Patent: May 25, 2010Assignee: The University of ManchesterInventors: David J. Clarke, Michael H. Harrison, Harmesh S. Aojula
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Patent number: 7709047Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: July 23, 2003Date of Patent: May 4, 2010Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 7704728Abstract: A microfluidic system employs a microchannel and a gravity driven pump comprising horizontally oriented fluid supply reservoirs which supplies fluid to the microchannel at a substantially constant rate. The device is useful for numerous microfluidic applications, for example in the culture and/or treatment of biological systems under constant flow-induced stress, cell-size sorting, motile sperm sorting, or embryo culture.Type: GrantFiled: July 18, 2002Date of Patent: April 27, 2010Assignee: The University of MichiganInventors: Shuichi Takayama, Joseph Chang, Dongeun Huh, Xiaoyue Zhu, Brenda Cho, Gary D. Smith