Abstract: The present invention is directed to C-terminal truncated ?-amylase polypeptides, nucleic acid sequences encoding the truncated ?-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated ?-amylase. Expression of the truncated ?-amylase in a plant cell results in enhanced accumulation of biologically-active ?-amylase, or enhanced secretion of the ?-amylase from the plant cell, compared to the accumulation or secretion of a full length ?-amylase.
Type:
Grant
Filed:
October 22, 2009
Date of Patent:
February 28, 2012
Assignee:
Syngenta Participations AG
Inventors:
Andrew DeBrecht, Aron Silverstone, Yan Zhang, Shengsheng Zhang, Xuejun Zhong
Abstract: A method of acylating the 3? position of anthocyanin using an enzyme that transfers an aromatic acyl group to a sugar at the 3? position of anthocyanin or a gene encoding the enzyme.