Abstract: A cell fixative composition for fixing the internal components of a cell without disrupting the cell surface components is disclosed. The composition contains a compound for preserving intracellular morphology, a compound for fixing internal components of the cell, a compound which facilitates transportation of components across the cellular membrane, and a compound for increasing permeability of cell membranes. Methods for analyzing cells which have been fixed using the composition are also disclosed.
Type:
Grant
Filed:
March 27, 1992
Date of Patent:
June 6, 1995
Assignee:
Ortho Diagnostic Systems Inc.
Inventors:
Mark C. Connelly, Utpal R. Chakraborty, Houston G. Brooks, Jr.
Abstract: A limulus test-positive plant glycolipid having the following properties:molecular weight: 8000.+-.1000 (SDS electrophoresis)number of phosphorus: not less than 1/mol.number of hexosamine: 6.+-.2/mol.number of fatty acid: 6.+-.2/mol is effective in the stimulation of osteogenesis, oviposition and shell strength improvement in animals. The glycolipid may be administered as a composition comprising an effective amount of said glycolipid in admixture with a pharmaceutically or veterinarily acceptable carrier, such that when administered to an animal, the immune system of said animal is favorably stimulated.