Patents Examined by Richard C. Ekstrom
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Patent number: 11492607Abstract: The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.Type: GrantFiled: January 5, 2018Date of Patent: November 8, 2022Assignee: ABIOCHEM BIOTECHNOLOGY CO., LTD.Inventors: Zhenhua Tian, Zhanbing Cheng, Chuanmin Sun
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Patent number: 11466277Abstract: Disclosed herein are chimeric polypeptides, including compositions thereof, expression vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors, and methods of the present invention are also useful in gene therapy techniques.Type: GrantFiled: September 24, 2020Date of Patent: October 11, 2022Assignee: The Scripps Research InstituteInventors: Carlos F. Barbas, Andrew Mercer, Brian M. Lamb, Thomas Gaj
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Patent number: 11459548Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.Type: GrantFiled: January 27, 2021Date of Patent: October 4, 2022Assignee: DSM IP ASSETS B.V.Inventors: Viktor Marius Boer, Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Catharina Petronella Antonia Maria Kolen
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Patent number: 11459594Abstract: Provided are a fructose-4-epimerase variant having tagatose conversion activity, and a method of producing tagatose using the same.Type: GrantFiled: September 27, 2019Date of Patent: October 4, 2022Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Mi Lee, Eul Soo Park, Il Hyang Park, Sun Mi Shin, Sung Jae Yang, Ran Young Yoon, Eun Jung Choi, Seong Bo Kim, Seung Won Park
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Patent number: 11441162Abstract: Disclosed herein, in some embodiments, are vectors encoding biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes), engineered cells comprising the vectors, and methods of using biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes) to produce fatty acid amides.Type: GrantFiled: March 6, 2020Date of Patent: September 13, 2022Assignee: Massachusetts Institute of TechnologyInventors: Christopher A. Voigt, Fang-Yuan Chang
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Patent number: 11441134Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering.Type: GrantFiled: December 21, 2020Date of Patent: September 13, 2022Assignee: Novozymes A/SInventors: Carsten Hoerslev Hansen, Yitong Liu, Steffen Kayser, Vibeke Skovgaard Nielsen
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Patent number: 11421214Abstract: The invention belongs to the technical field of green chemistry, and provides a novel system based on a new nitrile hydratase for highly efficient catalytic conversion of aliphatic dinitriles. The invention discloses a new application of nitrile hydratase using Rhodococcus erythropolis CCM 2595 in catalyzing aliphatic dinitrile. In particular, the enzyme can regioselectivity catalyze the formation of 5-cyanopyramides from adiponitrile with high reaction rate under mild reaction conditions, which provides a new method for the industrial production of 5-cyanopyramides.Type: GrantFiled: October 25, 2019Date of Patent: August 23, 2022Assignee: DALIAN UNIVERSITY OF TECHNOLOGYInventors: Changhai Liang, Li Wang, Shengxian Liu, Tongyi Dou, Changhao Cui
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Patent number: 11407986Abstract: The present invention relates to polypeptide having alpha-amylase activity. The present invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.Type: GrantFiled: December 29, 2016Date of Patent: August 9, 2022Assignee: Novozymes A/SInventors: Torsten Bak Regueira, Bitten Plesner, Thomas Holberg Blicher, Anne Dorte Houg, Sofia Arnehed, Lars Lehmann Hylling Christensen, Carsten Andersen
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Patent number: 11401536Abstract: The present invention provides a method of preparing D-psicose comprising the steps of (i) providing a mixture of D-psicose and D-fructose; and (ii) contacting the mixture of D-psicose and D-fructose with a probiotic microorganism that is capable of metabolizing D-fructose but not D-psicose and capable of converting D-fructose into L-lactic acid and subjecting the microorganism to culture conditions that allow fermentative removal of D-fructose from the mixture of D-psicose and D-fructose with concomitant production of L-lactic acid. Further provided are uses of a probiotic microorganism that is capable of metabolizing D-fructose but not D-psicose and capable of converting D-fructose into L-lactic acid for fermentative removal of D-fructose from a mixture of D-psicose and D-fructose. In particular, the probiotic microorganism is Lactobacillus rhamnosus GG or Saccharomyces boulardii.Type: GrantFiled: May 31, 2018Date of Patent: August 2, 2022Assignee: NGEE ANN POLYTECHNICInventors: Xingding Zhou, Ling Jie Stephanie Lee
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Patent number: 11401563Abstract: The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45% paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or ?-Glucoceramidase for the treatment of Gaucher's Disease. The unique glycosylation results in improved therapeutic efficacy—surprisingly even without mannose-6-phosphate that is common for CHO cell produced lysosomal proteins.Type: GrantFiled: March 17, 2016Date of Patent: August 2, 2022Assignee: eleva GmbHInventors: Paulina Dabrowska-Schlepp, Fode Benjamin, Andreas Busch, Holger Niederkrüger, Andreas Schaaf
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Patent number: 11390893Abstract: The present application relates to a method for extracting coenzyme Q10 and a phospholipid from a coenzyme Q10 fermentation bacterium powder. The method is characterized in that the fermentation bacterium powder of a coenzyme Q10 production strain is subjected to extraction with a mixed solvent of which the three-dimensional Hansen solubility parameter is between 21 and 23 (J/cm3)1/2 and the hydrogen bonding solubility parameter thereof is between 10 and 12 (J/cm3)1/2. The present invention can efficiently extract two products, namely coenzyme Q10 and a phospholipid, from the coenzyme Q10 fermentation bacterium powder; the process thereof is highly operable, easy to be industrialized, and can provide a product with high purity and yield, having great economic benefit.Type: GrantFiled: May 23, 2018Date of Patent: July 19, 2022Assignees: ZHEJIANG NHU CO. LTD., ZHEJIANG UNIVERSITY, HEILONGJIANG NHU BIOTECHNOLOGY CO. LTD., SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD.Inventors: Shenfeng Yuan, Yang Yao, Yi Min, Baishan Hu, Zhaofeng Chen, Yong Li, Qichuan Li, Yunxuan Luo, Jinyang Zhang, Xuguang Li, Shichun Tu
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Patent number: 11384349Abstract: The disclosure discloses a recombinant Bacillus subtilis engineered bacterium capable of efficiently expressing keratinase, and belongs to the technical fields of genetic engineering and fermentation engineering. The disclosure successfully constructs a genetically engineered bacterium B. subtilis WB600-pP43NMK-ker capable of efficiently expressing keratinase by using a keratinase gene from Bacillus licheniformis (BBE11-1) as a target gene, pP43NMK as an expression vector and B. subtilis WB600 as an expression host; and meanwhile, the disclosure conducts in-depth research on fermentation media and fermentation conditions when the engineered bacteria are configured as a production strain to produce keratinase to obtain a fermentation medium capable of increasing a yield of keratinase and an optimum process for fermentation production of keratinase.Type: GrantFiled: November 25, 2020Date of Patent: July 12, 2022Assignee: Jiangnan UniversityInventors: Juan Zhang, Zheng Peng, Jian Chen, Guocheng Du, Xinzhe Mao, Hengrui Zhou
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Patent number: 11377648Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6.Type: GrantFiled: April 8, 2019Date of Patent: July 5, 2022Assignee: NOVOZYMES A/SInventors: Tomoko Namoto, Takashi Nakanishi, Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
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Patent number: 11377632Abstract: The present invention provides a method for producing bacterial cells, which comprises, in a multi-stage liquid culture process for bacteria, culturing a bacterium in a liquid medium containing an antibiotic in a stage prior to the final stage, and then lowering the concentration of the antibiotic in the final stage to a level lower than that in the prior stage. Accordingly, bacterial cells can be produced with good productivity while suppressing the appearance of variants forming abnormal colonies among the bacteria obtained after culture.Type: GrantFiled: September 25, 2018Date of Patent: July 5, 2022Assignee: IDEMITSU KOSAN CO., LTD.Inventor: Yasuyuki Morishita
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Patent number: 11352592Abstract: Detergent compositions including polypeptide variants and methods of cleaning and/or treatment of surfaces using such compositions, and fabric treatment compositions including polypeptide variants. The compositions may include surfactants: anionic, nonionic and/or cationic.Type: GrantFiled: January 3, 2019Date of Patent: June 7, 2022Assignee: The Procter & Gamble CompanyInventors: Lars Beier, Lars Henrik Oestergaard, Annette Helle Johansen, Klaus Gori, Thomas Holberg Blicher, Gernot J. Abel, Steen Krogsgaard, Jens E Nielsen, Lars Giger, Neil Joseph Lant
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Patent number: 11352652Abstract: Provided is a novel method for producing 4-aminocinnamic acid from 4-nitrophenylalanine. This method comprises: converting 4-nitrophenylalanine into 4-nitrocinnamic acid; and converting 4-nitrocinnamic acid into 4-aminocinnamic acid.Type: GrantFiled: March 4, 2019Date of Patent: June 7, 2022Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Naoki Takaya, Shunsuke Masuo, Yukie Kawasaki, Hajime Minakawa
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Patent number: 11345921Abstract: Provided are modified Gram-negative bacteria having an increased periplasmic volume. Also provided are methods of expressing exogenous genes in the bacteria and targeting protein production to the periplasmic space.Type: GrantFiled: February 2, 2021Date of Patent: May 31, 2022Assignee: GELTOR, INC.Inventor: Nikolay Ouzounov
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Patent number: 11345907Abstract: Described herein is a method of producing a drimane sesquiterpene, such as an albicanol compound and/or derivatives thereof, by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide of the Haloacid dehalogenase-like (HAD-like) hydrolase superfamily as obtainable from plants of the genus Dryopteris, in particular of the species Dryopteris fragrans. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. Also described herein are host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as an albicanol compound.Type: GrantFiled: May 28, 2019Date of Patent: May 31, 2022Assignee: Firmenich SAInventors: Didier Belorgey, Xiu-Feng He, Qi Wang, Lily Ji-Xiu Zhang
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Patent number: 11332731Abstract: The disclosure discloses a nitrile hydratase mutant, a genetically engineered bacterium containing the mutant and applications thereof, and belongs to the technical field of enzyme engineering. In the disclosure, glycine at position 47 of a nitrile hydratase mutant ?L6T/A19V/F126Y-?M46K/E108R/S212Y (disclosed in the patent of disclosure CN102216455A) is mutated to asparagine. The obtained new mutant enzyme has better temperature tolerance and tolerance to a product, and is conducive to future industrial production. The recombinant strain containing the nitrile hydratase mutant is fermented at high density, and 3-cyanopyridine is used as a substrate to carry out a whole-cell catalytic reaction to prepare nicotinamide. Compared with a chemical production method, the method has a safe and clean production process and no environmental pollution. Compared with an enzymatic method, the substrate price is cheap and the catalytic efficiency is high.Type: GrantFiled: March 22, 2021Date of Patent: May 17, 2022Assignee: JIANGNAN UNIVERSITYInventors: Zhemin Zhou, Zhongmei Liu, Junling Guo, Li Zhou, Wenjing Cui, Yao Lan
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Patent number: 11332764Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.Type: GrantFiled: October 15, 2020Date of Patent: May 17, 2022Assignee: Viridos, Inc.Inventors: Imad Ajjawi, Eric R. Moellering, Leah Soriaga, Moena Aqui