Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
Type:
Grant
Filed:
October 3, 2013
Date of Patent:
March 14, 2017
Assignee:
Roal Oy
Inventors:
Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Jamsa, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
Abstract: The present invention relates to the development of an L-arabinose isomerase variant from Thermotoga neapolitana DSM 5068, which is a kind of thermophile, on the basis of protein molecular modeling. Moreover, the present invention relates to a method of producing D-tagatose from D-galactose by using the enzyme or a microorganism of the genus Corynebacterium expressing the enzyme.
Type:
Grant
Filed:
April 25, 2014
Date of Patent:
March 14, 2017
Assignee:
CJ Cheiljedang Corporation
Inventors:
In Seok Oh, Chang Gyeom Kim, Seung Hyun Cho, Seong Bo Kim, Yang Hee Kim, Kyong Yeon Cho, Sung Jae Yang, Jin Ha Kim
Abstract: Antiviral compositions comprising a modified nuclease, or a plurality of such modified nucleases having at least one non-natural amino acid residue substituted for a naturally occurring amino acid in a parent nuclease are provided, as are methods of use and kits providing unit dosages of such compositions.
Abstract: A mutant MMLV reverse transcriptase that may have an improvement in one or more properties is provided. For example, the present reverse transcriptase is believed to be more efficient relative to other commercially available MMLV reverse transcriptase variants, particularly for templates with a higher GC content.
Type:
Grant
Filed:
September 23, 2016
Date of Patent:
February 28, 2017
Assignee:
New England Biolabs, Inc.
Inventors:
Yan Xu, Jennifer Ong, Shengxi Guan, Nicole Nichols
Abstract: The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant.
Type:
Grant
Filed:
March 5, 2013
Date of Patent:
February 14, 2017
Assignee:
ARES TRADING S.A.
Inventors:
Hyun Sook Jang, Dong Heon Lee, Sun Kyu Kim, Yong Ho Ahn, Sang Kyung Park
Abstract: The present invention relates to methods and compositions for increasing production of methyl ketones in a genetically modified host cell that overproduces ?-ketoacyl-CoAs through a re-engineered ?-oxidation pathway and overexpresses FadM.
Type:
Grant
Filed:
October 26, 2012
Date of Patent:
January 31, 2017
Assignee:
The Regents of the University of California
Abstract: Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.
Type:
Grant
Filed:
August 12, 2013
Date of Patent:
January 10, 2017
Assignee:
Danisco US Inc.
Inventors:
Su Yin Marina Chow, Thomas P. Graycar, Jacquelyn A. Huitink, Casper Vroemen, David L. Wong
Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Daldinia caldariorum.
Type:
Grant
Filed:
October 8, 2013
Date of Patent:
January 3, 2017
Assignee:
Novozymes A/S
Inventors:
Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
Abstract: The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.
Type:
Grant
Filed:
February 7, 2012
Date of Patent:
January 3, 2017
Assignee:
BIONEER CORPORATION
Inventors:
Han Oh Park, Sung Jun Yang, Sung Mo Joo, Byoung Oh Hwang
Abstract: The present invention refers to novel dimeric proteins obtained from modified ubiquitin capable of binding targets with high affinity. The novel dimeric binding proteins comprise a combination of amino acid substitutions and at least one insertion of amino acids in one of the monomers. The invention is further directed to the use of said proteins in medical diagnosis or treatment methods.
Type:
Grant
Filed:
June 15, 2012
Date of Patent:
November 15, 2016
Assignee:
Scil Proteins GmbH
Inventors:
Joerg Nerkamp, Eva Bosse-Doenecke, Arnd Steuernagel, Ulrike Fiedler, Markus Fiedler
Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
Type:
Grant
Filed:
February 18, 2015
Date of Patent:
November 15, 2016
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Andrew C. Eliot, Caroline M. Peres, Dmitrii V. Vaviline
Abstract: The invention relates to new method of peptide hydrolysis, in particular of the cell walls of Gram-positive bacteria, wherein the active form of LytM or derivative thereof is contacted with a peptide substrate, preferably with the cell walls of Gram-positive bacteria, in an aqueous environment of conductivity lower than 10 mS/cm. The invention also relates to composition comprising active form of LytM or derivative thereof and new uses of active form of LytM or derivative thereof.
Type:
Grant
Filed:
October 17, 2013
Date of Patent:
November 8, 2016
Assignee:
MIEDZYNARODOWY INSTYTUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ
Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.
Type:
Grant
Filed:
May 15, 2015
Date of Patent:
November 8, 2016
Assignee:
REGENTS OF THE UNIVERSITY OF MICHIGAN
Inventors:
David H. Sherman, Michael Marie Kaufman-Schofield, Sunit Jain, Gregory Dick
Abstract: The invention provides for nucleotide sequences encoding for a chimeric sulfatase, viral vectors expressing such sequences for gene therapy and pharmaceutical uses of the chimeric expressed protein. The invention is particularly applied in the therapy of mucopolysaccharidosis, preferably type IIIA.
Abstract: Methods and compositions relating to the engineering of an improved protein with methionine-?-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.
Type:
Grant
Filed:
August 29, 2014
Date of Patent:
November 1, 2016
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Inventors:
George Georgiou, Everett Stone, Wei-Cheng Lu
Abstract: This invention provides methods and systems for the production of butanol. Specifically, the methods and systems of the present invention use syntrophic co-cultures for the production of butanol from syngas.
Type:
Grant
Filed:
January 18, 2013
Date of Patent:
October 18, 2016
Assignee:
SYNATA BIO, INC.
Inventors:
Mike Enzien, Rathin Datta, Richard Tobey
Abstract: The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed.
Type:
Grant
Filed:
June 6, 2013
Date of Patent:
October 4, 2016
Assignee:
Roal Oy
Inventors:
Susanna Makinen, Kari Juntunen, Alexandra Komander, Kim Langfelder, Jari Vehmaanpera, Terhi Puranen
Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).
Type:
Grant
Filed:
July 12, 2012
Date of Patent:
September 20, 2016
Assignees:
KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.
Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid.
Type:
Grant
Filed:
October 3, 2015
Date of Patent:
August 30, 2016
Assignee:
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventors:
Sang Yup Lee, Sung Won Lim, Hyohak Song