Abstract: This Invention discloses a method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt by microbial fermentation. The method is intended to manufacture N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt in higher efficiency and higher yield, by expression of vitreoscilla hemoglobin in microorganism.
Abstract: The invention provides an improved method of isolating the 3-(E)-isomer of an unsaturated carboxylic acid from a mixture of corresponding (E/Z)isomers. More particularly, the present invention relates to an improved method for the biocatalytic preparation of (3E,7E)-homofarnesylic acid; as well as a novel biocatalytic method for the improved preparation of homofarnesol, in particular of (3E,7E)-homofarnesol and homofarnesol preparations having an increased content of (3E,7E)-homofarnesol. The present invention also relates to methods of preparing(?)-ambroxby applying (3E,7E)-homofarnesylic acid or (3E,7E)-homofarnesol as obtained according to the invention as starting material.
Type:
Grant
Filed:
February 23, 2018
Date of Patent:
October 5, 2021
Assignee:
BASF SE
Inventors:
Wolfgang Siegel, Melanie Weingarten, Michael Breuer, Mathias Schelwies
Abstract: A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having ?, ?-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes.
Type:
Grant
Filed:
July 4, 2016
Date of Patent:
September 21, 2021
Assignee:
The Governing Council of The University of Toronto
Inventors:
Alexander Yakunin, Anna Khusnutdinova, Jeong Chan Joo, Radhakrishnan Mahadevan
Abstract: The present disclosure relates to a novel chitinolytic enzyme, particularly to a chitinolytic enzyme including an exo-?-N-acetylglucosaminidase (Clocel_3193) constituting a cellulosome derived from Clostridium cellulovorans as an active ingredient. The present disclosure allows utilization of chitin biomass which has not been used formerly as a raw material and allows environment-friendly production of N-acetylglucosamine. In addition, since the Clocel_3193 has a cell wall binding ability and degrading ability, the Clocel_3193 may be used in an antifungal composition.
Type:
Grant
Filed:
July 7, 2020
Date of Patent:
September 21, 2021
Assignee:
Korea University Research and Business Foundation
Abstract: Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.
Type:
Grant
Filed:
June 4, 2019
Date of Patent:
September 21, 2021
Assignees:
UNIVERSITY OF MARYLAND, BALTIMORE, UNIVERSITY OF WASHINGTON
Inventors:
Robert K. Ernst, Mark Pelletier, Adeline Hajjar
Abstract: The present invention relates to the preparation of amines from aldehydes and ketones by reductive amination with enzymes having a reductive aminase activity on aldehydes and ketones devoid of any carboxyl group gamma of the carbonyl group. The invention also relates to the enzymes per se and their uses in biocatalysis. The enzymes are derived from Mycobacterium smegmatis and vaccae, Cystobacter fuscus, Microbacterium sp. and Aminomonas paucivorans.
Type:
Grant
Filed:
July 5, 2018
Date of Patent:
September 14, 2021
Assignees:
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, UNIVERSITE D'EVRY VAL D'ESSONNE (UEVE)
Abstract: This invention claims processes that append a single nucleotide having a 3?-ONH2 moiety to the 3?-ends of an oligonucleotide primer using 3?-deoxynucleoside triphosphates that have, instead of a 3?-OH moiety, a 3?-ONH2 moiety, where the nucleotides contain both standard and non-standard nucleobases, and where as a key claim limitation, substantially no hydroxylamine is present in the solutions used in the claimed processes.
Abstract: The present invention relates to methods and compositions for modulating glycosylation profile, such as the galactosylation profile, of recombinant proteins expressed by mammalian host cells during the cell culture process by supplementing cell culture media with a peracetyl galactose.
Type:
Grant
Filed:
May 10, 2017
Date of Patent:
September 14, 2021
Assignee:
ARES TRADING S.A.
Inventors:
Martin Jordan, Gabrielle Leclercq, Jonathan Souquet, Herve Broly, David Bruhlmann
Abstract: The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume gluconse and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
September 7, 2021
Assignee:
CARGILL, INCORPORATED
Inventors:
Arlene M. Fosmer, Christopher K. Miller, Gregory Michael Poynter, Brian Jeffrey Rush, Jon Veldhouse
Abstract: Methods for increasing carbon-based chemical product yield in an organism by genetically modifying one or more genes involved in a stringent response and/or in a regulatory network, nonnaturally occurring organisms having increased carbon-based chemical product yield, and methods for use in production of carbon-based chemical products are provided.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
August 24, 2021
Assignee:
INV Nylon Chemicals Americas, LLC
Inventors:
Alexander Brett Foster, Jonathan Kennedy
Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Type:
Grant
Filed:
August 30, 2018
Date of Patent:
August 24, 2021
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, Yang Luo, Yilin Wu, Beihua Zhang, David Byun, Xiaodan Yu
Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Type:
Grant
Filed:
December 9, 2019
Date of Patent:
August 24, 2021
Assignee:
Codexis, Inc.
Inventors:
Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier
Abstract: An enzyme having the following characteristics (a) and (b): (a) the enzyme has an activity of reversible dehydrogenation of D-amino acids; (b) the enzyme is a hexamer of polypeptides having an amino acid sequence having 80% or greater identity to the amino acid sequence of SEQ ID NO: 2.
Type:
Grant
Filed:
August 9, 2018
Date of Patent:
August 24, 2021
Assignees:
KUREHA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Abstract: The disclosure relates to the field of fusion proteins. In some aspects, the disclosure relates to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosure relates to corn-pounds produced by artificial cytochrome P450 enzymes.
Type:
Grant
Filed:
October 26, 2017
Date of Patent:
August 3, 2021
Assignee:
University of Florida Research Foundation, Incorporated
Abstract: The current disclosure provides a process for enzymatically converting a saccharide into allulose. The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase (A6PP).
Type:
Grant
Filed:
December 14, 2017
Date of Patent:
August 3, 2021
Assignee:
BONUMOSE, INC.
Inventors:
Daniel Joseph Wichelecki, Edwin O. Rogers
Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Type:
Grant
Filed:
March 3, 2020
Date of Patent:
August 3, 2021
Assignee:
Codexis, Inc.
Inventors:
Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
Abstract: The present invention relates to alpha-amylase variants with improved stability in the presence of glucose and/or relatively higher activity on long chain versus short chain substrates. The present encoding invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Abstract: The present invention relates to an expansin-agarase enzyme complex and a method of degrading agar by using the same. The use of the enzyme complex according to the present invention can efficiently degrade agar obtained from marine biomass, and thus can efficiently provide not only galactose or glucose, necessary for ethanol production, but also useful biologically active substances, such as diose, triose, and oligosaccharides.
Type:
Grant
Filed:
January 2, 2018
Date of Patent:
July 27, 2021
Assignee:
Korea University Research and Business Foundation
Inventors:
Sung Ok Han, Da-woon Jeong, Jeong-Eun Hyeon
Abstract: The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.