Patents Examined by Samuel Liu
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Patent number: 7960519Abstract: A method of treating biologic material with a composition containing at least one fatty acid derivative and/or at least one fatty alcohol, a buffer, and a salt to result in protein partitioning into an aqueous phase and an organic phase. The protein may be a membrane protein. The cells may be plant cells and/or animal cells. The proteins can be thereafter be used for downstream applications.Type: GrantFiled: May 30, 2006Date of Patent: June 14, 2011Assignee: Pierce Biotechnology, Inc.Inventors: Valerie J. O'Sullivan, Edward J. Conklin
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Patent number: 7939647Abstract: An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had ?-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic properties as high as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes.Type: GrantFiled: November 21, 2008Date of Patent: May 10, 2011Assignee: National Institute of Agrobiological SciencesInventors: Takahiro Kikawada, Takashi Okuda, Masahiko Watanabe, Kazuei Mita, Keiko Kadono
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Patent number: 7928209Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: December 17, 2007Date of Patent: April 19, 2011Assignee: Eisai R & D Management Co. Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7923430Abstract: Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.Type: GrantFiled: October 11, 2005Date of Patent: April 12, 2011Assignee: Glycofi, Inc.Inventor: Tillman U. Gerngross
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Patent number: 7919609Abstract: Novel bacterial insecticidal proteins and equivalents thereof were isolated. These proteins and the DNA sequences encoding them are useful to make insecticidal compositions or transgenic plants to protect plants from damage by insects, particularly coleopteran insects.Type: GrantFiled: August 10, 2006Date of Patent: April 5, 2011Assignee: Bayer BioScience N.V.Inventors: Annemie Boets, Greta Arnaut, Jeroen Van Rie, Nicole Damme
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Patent number: 7910714Abstract: Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use.Type: GrantFiled: January 19, 2010Date of Patent: March 22, 2011Assignees: The University of Chicago, The University of WashingtonInventors: Benjamin S. Glick, Brooke Bevis, Daniel E. Strongin, David Baker, Michelle Scalley-Kim
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Patent number: 7910712Abstract: The present invention provides isolated DNA encoding a GWT1 protein having activity to confer resistance of a fungus against a compound of formula Ia, and wherein a defect of a function of the GWT1 protein leads to a decrease in the amount of a glycosylphosphatidylinositol (GPI)-anchored protein in the cell wall of a fungus.Type: GrantFiled: April 15, 2008Date of Patent: March 22, 2011Assignee: Eisai R&D Management Co., LtdInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7906636Abstract: The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.Type: GrantFiled: November 13, 2009Date of Patent: March 15, 2011Assignee: The Regents of the University of CaliforniaInventors: Roger Y. Tsien, Robert E. Campbell, Nathan C. Shaner
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Patent number: 7906624Abstract: A multimeric peptidomimetic that comprises two or more monomers is disclosed. The monomers comprise an exocyclic peptide comprising a ring structure, a flexible linker sequence and a multimeric motif. Use of the monomers, a nucleic acid molecule encoding monomers, recombinant expression vectors comprising the nucleic acid molecule and host cells comprising a recombinant expression vector are disclosed. Methods of delivering a drug, a toxin, a nucleic acid molecule, a radionuclide or a detectable compound to a cell are disclosed.Type: GrantFiled: February 18, 2005Date of Patent: March 15, 2011Assignee: The Trustees of the University of PennsylvaniaInventors: Mark I. Greene, Ramachandran Murali, Hongtao Zhang
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Patent number: 7906146Abstract: Novel formulations containing exendins, exendin agonists and/or exendin analogs are provided.Type: GrantFiled: February 20, 2009Date of Patent: March 15, 2011Assignee: Amylin Pharmaceuticals, Inc.Inventors: Orville G. Kolterman, Andrew A. Young, James J. L'Italien
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Patent number: 7902145Abstract: Methods of sterilizing dermal fillers and injectable collagen material have been developed which reduce the level of active biological contaminants or pathogens without adversely affecting the material, i.e., wherein the dermal fillers and injectable collagen material retain their same properties before and after its terminal sterilization. In one embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation contains the steps of protecting the filler or material from radiation, and irradiating the filler or material with a suitable dose of radiation for a time and at a rate effective to sterilize the filler or injectable material. In a preferred embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation includes the steps of a) freezing the filler or material at a temperature below its freezing temperature, which is generally below 0° C.Type: GrantFiled: May 17, 2006Date of Patent: March 8, 2011Assignee: Albiorex, LLCInventors: George Chu, C. Randall Harrell, Hector J. Gomez
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Patent number: 7897387Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: April 1, 2008Date of Patent: March 1, 2011Assignee: Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7892755Abstract: The present invention provides a method of screening a compound or its salt that alters binding properties of a protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 1, or a salt thereof, and a ligand capable of specifically binding to the protein or its salt, which comprises using (a) the protein, its partial peptide, or a salt thereof and (b) the ligand; a screening kit therefor, and so on. The screening method and kit of the present invention are useful for screening an agent for the prevention/treatment of, e.g., digestive tract disorders, cancer, immune disorders, type II diabetes mellitus or obesity, etc.Type: GrantFiled: August 29, 2005Date of Patent: February 22, 2011Assignee: Takeda Pharmaceutical Company LimitedInventors: Yasuaki Ito, Kazunori Nishi, Shoichi Ohkubo, Masataka Harada
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Patent number: 7888314Abstract: The present invention relates to methods of treating intermittent claudication comprising administering glucagon-like peptide-1 (GLP-1) molecules to subjects suffering therefrom.Type: GrantFiled: August 5, 2005Date of Patent: February 15, 2011Assignee: Amylin Pharmaceuticals, Inc.Inventors: David R. Hathaway, Thomas R. Coolidge
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Patent number: 7868138Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: February 1, 2008Date of Patent: January 11, 2011Assignee: Codexis, Inc.Inventors: Willem P. C. Stemmer, Andreas Crameri
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Patent number: 7851604Abstract: Genes each encoding a novel transcriptional regulator having a bromodomain have been successfully isolated from a human testis cDNA library using primers prepared based on an EST sequence found using the bromodomain sequence of the transcriptional regulator. These genes are structurally analogous to each other.Type: GrantFiled: February 22, 2007Date of Patent: December 14, 2010Assignee: Chugai Seiyaku Kabushiki KaishaInventor: Michael H. Jones
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Patent number: 7833972Abstract: A method of suppressing the differentiation of an adipocite and/or lipid accumulation in an adipocyte, which comprises contacting the adipocyte with a protein comprising the amino acid sequence of SEQ ID NO: 2 or a salt thereof, or with a polynucleotide comprising a sense strand sequence encoding a protein comprising the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: February 13, 2009Date of Patent: November 16, 2010Assignee: Takeda Pharmaceutical Company LimitedInventors: Yuji Matsuzawa, Tohru Funahashi, Iichirou Shimomura, Naoki Furuyama
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Patent number: 7829072Abstract: A hypoallergenic cleansing, cosmetic, conditioning or dermatological composition for treating skin or hair is provided which contains serum albumin in an amount effective to achieve cleansing, conditioning, wound debrisment, or other beneficial cosmetic or dermatological purpose for skin or hair, along with a suitable cleansing, conditioning, cosmetic, antibacterial or dermatological agent, vehicle, carrier or excipient. The compositions may be in any suitable form for treating skin or hair, such as a soap, shampoo, cream, oil, lotion, gel, gel-based ointment, and the like. The serum albumin compositions are preferably prepared using human serum albumin produced by recombinant means, and such compositions are useful in that they allow the albumin to be absorbed in the surface of skin or hair so as to replenish the structure of these tissues when utilized as a cleansing, cosmetic or dermatological agent.Type: GrantFiled: December 21, 2000Date of Patent: November 9, 2010Inventor: Daniel C. Carter
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Patent number: 7829684Abstract: Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.Type: GrantFiled: August 14, 2009Date of Patent: November 9, 2010Assignee: Genentech, Inc.Inventors: James A. Ernst, Daniel Yansura, Hok Seon Kim
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Patent number: 7825083Abstract: A composite tissue formed in situ is provided. The composite tissue includes a synovial joint tissue; and a barrier material adhered thereto for sealing the synovial joint tissue against synovial fluid. Also provided is a method for regenerating synovial joint tissue in situ by excluding synovial fluid therefrom. The method includes providing a synovial joint tissue having a defect; and placing a barrier material in intimate contact with the defect for sealing the defect against synovial fluid. The barrier material includes a curable protein copolymer. The method further includes curing the protein copolymer in situ. The barrier material can include a crosslinked network, or a self gelled network of repeating elastin-like and fibroin-like polymer chains.Type: GrantFiled: February 10, 2005Date of Patent: November 2, 2010Assignee: Spine Wave, Inc.Inventor: Andrew Carter