Patents Examined by Samuel W. Liu
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Patent number: 11530235Abstract: Mutant mono ADP-ribose-polymerases (mono-PARP) proteins and small molecule compound substrates specific for the mutant mono-PARP proteins as well as methods of using these compositions to identify protein targets of the mono-PARPs and to screen for antagonists of the mono-PARPs are described.Type: GrantFiled: December 16, 2019Date of Patent: December 20, 2022Assignee: Oregon Health & Science UniversityInventors: Michael Cohen, Ian Carter-O'Connell, Rory Morgan, Haihong Jin
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Patent number: 11524984Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence compriseType: GrantFiled: June 29, 2017Date of Patent: December 13, 2022Assignee: SPIBER TECHNOLOGIES ABInventors: Anna Rising, Jan Johansson, Marlene Andersson
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Patent number: 11472864Abstract: Compositions and methods relating to ApoA-1 fusion polypeptides are disclosed. The fusion polypeptides include a first polypeptide segment corresponding to an ApoA-1 polypeptide or ApoA-1 mimetic, and may also include a dimerizing domain such as, e.g., an Fc region, which is typically linked carboxyl-terminal to the first polypeptide segment via a flexible linker. In some embodiments, the fusion polypeptide further includes a second polypeptide segment located carboxyl-terminal to the first polypeptide segment and which confers a second biological activity (e.g., an RNase, paraoxonase, platelet-activating factor acetylhydrolase, cholesterol ester transfer protein, lecithin-cholesterol acyltransferase, polypeptide that specifically binds to proprotein convertase subtilisin/kexin type 9, or polypeptide that specifically binds to amyloid beta). Also disclosed are dimeric proteins comprising first and second ApoA-1 fusion polypeptides as disclosed herein.Type: GrantFiled: December 3, 2021Date of Patent: October 18, 2022Assignee: Theripion, Inc.Inventors: Martha S. Hayden-Ledbetter, Jeffrey A. Ledbetter
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Patent number: 11466073Abstract: A serum albumin variant, or functional fragment thereof, comprising one or more amino acid substitutions selected from (i) glycine, isoleucine, lysine, methionine, phenylalanine, tryptophan, tyrosine, valine and leucine substituted for glutamine at position (522); (ii) valine substituted for alanine at position (552); and (iii) alanine, glutamic acid, histidine, serine, lysine and arginine substituted for glycine at position (572).Type: GrantFiled: October 18, 2018Date of Patent: October 11, 2022Assignee: CSL LimitedInventors: Sabine Rauth, Andrew Hammet, Kirsten Edwards, Con Panousis
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Patent number: 11396537Abstract: The present invention relates to a polypeptide, a process for the production thereof and a use thereof. The polypeptide of the present invention has excellent adhesion effect and is highly stable in an aqueous solution. Endotoxins can be more easily removed from the product of the polypeptide.Type: GrantFiled: November 27, 2019Date of Patent: July 26, 2022Assignee: Shanxi Jinbo Bio-Pharmaceutical Co., Ltd.Inventor: Xia Yang
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Patent number: 11359183Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.Type: GrantFiled: May 23, 2018Date of Patent: June 14, 2022Assignee: Codexis, Inc.Inventors: Scott J. Novick, Nikki Dellas, Oscar Alvizo, Ravi David Garcia, Charlene Ching, David Entwistle
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Patent number: 11345897Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: May 31, 2022Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Patent number: 11332723Abstract: The present disclosure provides methods of producing commodity products, the methods involving culturing a host cell that is genetically modified to produce a uronate dehydrogenase (UDH) that converts a sugar acid to its corresponding 1,5-aldonolactone, that uses NADP+ or NAD+ as a cofactor, and that produces NADPH or NADH, respectively, where the host cell coexpresses an endogenous or a heterologous reductase that utilizes the produced NADPH or NADH to generate the commodity product or a precursor thereof. The present disclosure provides a method of producing downstream products of glycerol and pyruvate in a genetically modified microbial host cell, the method involving culturing a genetically modified microbial host cell of the present disclosure in a culture medium comprising D-galacturonic acid. The present disclosure provides variant UDH polypeptides that utilize NADP+, nucleic acids encoding the variant UDH polypeptides; and host cells genetically modified with the nucleic acids.Type: GrantFiled: October 2, 2017Date of Patent: May 17, 2022Assignee: The Regents of the University of CaliforniaInventors: John E. Dueber, Ryan Joseph Protzko
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Patent number: 11326157Abstract: Methods and compositions for improving the genome-wide specificities of targeted base editing technologies.Type: GrantFiled: May 25, 2018Date of Patent: May 10, 2022Assignee: The General Hospital CorporationInventors: J. Keith Joung, Jason Michael Gehrke
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Patent number: 11306126Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.Type: GrantFiled: April 1, 2019Date of Patent: April 19, 2022Assignee: SPIBER INC.Inventors: Kaori Sekiyama, Mizuki Ishikawa, Shinya Murata
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Patent number: 11292827Abstract: Disclosed is a method of producing high-concentration collagen for use as a medical material, including: washing tissue of a mammal; subjecting the washed tissue to crushing and immersion in ethyl alcohol; subjecting the tissue to enzymatic treatment with stirring in purified water containing phosphoric acid and pepsin; adding sodium chloride to the collagen subjected to enzymatic treatment, performing stirring, and aggregating collagen; dissolving the aggregated collagen in purified water to give a collagen solution, which is then filtered using a filter and concentrated by removing the pepsin, low-molecular-weight material, and sodium chloride from the collagen solution using a tangential flow filtration device; subjecting the concentrated collagen to sterile filtration, aggregating the collagen using a pH solution in a neutralization tank, and concentrating the collagen by removing a non-aggregated solution; and concentrating the concentrated collagen using a centrifuge and stirring the concentrated collagType: GrantFiled: December 11, 2014Date of Patent: April 5, 2022Assignee: SEWONCELLONTECH CO., LTD.Inventors: Cheong Ho Chang, Hyeong Woo Jeong, Ji Chui Yoo, Se Ken Yeo, Dong Sam Suh
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Patent number: 11274320Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.Type: GrantFiled: October 23, 2020Date of Patent: March 15, 2022Assignee: Ginkgo Bioworks, Inc.Inventors: Kim Cecelia Anderson, Elena Brevnova, Dylan Alexander Carlin, Brian Carvalho, Nicholas Flores, Katrina Forrest, Massimo Merighi, Gabriel Rodriguez, Emily E. Wrenbeck
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Patent number: 11225645Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase, According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: January 18, 2022Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Patent number: 11213613Abstract: Provided is a three dimensional tissue scaffold comprising a stem cell attracting element associated with a matrix, and fusion protein of stem cell attracting factor and collagen-binding domain, and methods of uses thereof.Type: GrantFiled: September 30, 2015Date of Patent: January 4, 2022Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Jianwu Dai, Jie Sun, Bing Chen, Zhifeng Xiao
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Patent number: 11162086Abstract: Lipase enzymes, methods of making lipase enzymes, methods of using lipase enzymes in food, feed, personal care, detergents, grain processing, pulp and paper processing, biofuels, ethanol production, textiles, dairy processing, cocoa butter processing, cocoa extraction, dietary supplements, coffee processing, coatings, water treatment, and oil processing.Type: GrantFiled: May 10, 2018Date of Patent: November 2, 2021Assignee: BASF SEInventors: Michael Liszka, Jochen Kutscher, Aditi Prashar, Cristina Pop
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Patent number: 11155789Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: October 26, 2021Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Patent number: 11130973Abstract: The present disclosure relates to the technical fields of genetic engineering and microbial fermentation, and discloses a recombinant strain for producing L-lactic acid, wherein the recombinant strain is obtained by genetically modifying a starting strain, the activity of D-lactate dehydrogenase of the recombinant strain is weakened or inactivated, and the activity of L-lactate dehydrogenase is enhanced, as compared with the starting strain. The present invention can significantly improve the fermentation effect, the yield of the L-lactic acid and the optical purity of the product L-lactic acid.Type: GrantFiled: December 29, 2020Date of Patent: September 28, 2021Assignees: COFCO (JILIN) BIO-CHEMICAL TECHNOLOGY CO., LTD, NUTRITION & HEALTH RESEARCH INSTITUTE, COFCO CORPORATIONInventors: Yi Tong, Yi Li, Yuan Zhang, Bo Chen, Xiaoyan Wang, Tai An, Kai Yang
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Patent number: 11098337Abstract: The disclosure relates to the field of glyco-engineering, more specifically, to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells are glyco-engineered yeast cells in which, additionally, at least one exogenous enzyme needed for complex glycosylation is present, e.g., allowing easier separation of differentially glycosylated glycoproteins.Type: GrantFiled: July 2, 2014Date of Patent: August 24, 2021Assignees: VIB VZW, Universiteit GentInventors: Nico L. M. Callewaert, Karen De Pourcq, Steven Geysens, Leander Meuris
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Patent number: 11066656Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.Type: GrantFiled: June 25, 2020Date of Patent: July 20, 2021Assignee: Halozyme, Inc.Inventors: Ge Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
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Patent number: 11066682Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.Type: GrantFiled: April 2, 2012Date of Patent: July 20, 2021Assignee: Genomatica, Inc.Inventors: Derek L. Greenfield, Louis G. Hom, Fernando A. Sanchez-Riera, Zhihao Hu, Vikranth Arlagadda, Eli S. Groban, Scott A. Frykman