Abstract: Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

Abstract: The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a recombinant microorganism converting glycerol into 1,3-propanediol and overexpressing the hcpR and/or frdX gene on a medium comprising glycerine. A recombinant microorganism for the production of 1,3 propanediol from glycerol, wherein said microorganism converts glycerol into 1,3-propanediol and 10 overexpresses the hcpR and/or the frdX gene.

Abstract: Methods and kits for predicting infusion reaction risk and antibody-mediated loss of response during intravenous PEGylated uricase therapy in gout patients is provided. Routine SUA monitoring can be used to identify patients receiving PEGylated uricase who may no longer benefit from treatment and who are at greater risk for infusion reactions.

Abstract: The present invention provides a consortium of resistant microorganisms for biodegrading carbohydrates in the organic fraction of any source of solid waste. The genetic, metabolic and morphological structure of these resistant microorganisms works efficiently by biodegrading and mineralising solid biodegradable waste from municipal or harvest solid waste, reducing the production of gases and leachates. This compound enriches and increases the concentration of beneficial microorganisms, generating high-quality biological fertilisers suitable for use in agricultural production, in land recovery and conservation, under the parameters established in sustainable organic farming, which seeks to conserve, recover and use nature or the environment without generating the least negative impact.

Abstract: The disclosure relates to a mixture of bacteria belonging to at least six or seven different and specific bacterial species preferably for use in preventing or treating gastro-intestinal disorders. Preferably, the mixture of bacteria is grown together in a fermenter prior to administering the mixture to a subject in order to prevent or treat the disorder.

Abstract: A method of treatment is disclosed, comprising administering a composition of Cyclodextrin and reduced, nanonized L-Glutathione to a patient in need of treatment, wherein the L-Glutathione molecule is non-acetylated, non-Esterified, and non-fatty acid attached.

Abstract: Methods and kits for predicting infusion reaction risk and antibody-mediated loss of response during intravenous PEGylated uricase therapy in gout patients is provided. Routine SUA monitoring can be used to identify patients receiving PEGylated uricase who may no longer benefit from treatment and who are at greater risk for infusion reactions.

Abstract: The disclosure relates to a mixture of bacteria belonging to at least six or seven different and specific bacterial species preferably for use in preventing or treating gastro-intestinal disorders. Preferably, the mixture of bacteria is grown together in a fermenter prior to administering the mixture to a subject in order to prevent or treat the disorder.

Abstract: The aim of the invention is to provide a method for biotechnological production of carboxylic acids, in which the acid-forming micro-organisms are cultured in an unsterile manner in a submerged phase containing waste water containing all carbon and nutrient medium components necessary for the production of the carboxylic acid, which method avoids the disadvantages of known methods and enables high product concentrations and productivity while at the same time the resources of water and power are being conserved. This aim is achieved, according to the invention, in that micro-organisms are used that are cultured under unsterile conditions in a culture medium containing waste water with the addition of carbon-rich compounds.

Abstract: Devices and methods for delivering oxygen and other therapeutic gases to a target, such as a tissue, a tissue-engineered construct, and a wound, in a controlled and sustained manner are disclosed.

Abstract: In certain aspects, provided herein are methods and kits for detecting the presence of C. difficile in a biological sample. Also provided herein are methods of selecting of identifying a subject that would benefit from C. difficile treatment.

Abstract: Methods of detecting biologicals in samples is provided herein. The detection is based on the formation of aggregates. The disclosed compositions include labeling particles and/or aggregating particles. The labeling particles and the aggregating particles may each include a receptor bound to the particle. The receptor can be either directly attached to the particle or indirectly attached to the particle through a linker. One method of detection may be visual and another may include advanced quantification of the formed aggregates.

Abstract: The present invention relates to Candida strains comprising a mutation or deletion in the first and/or second XYL2 allele which can be used for producing one or more sugar alcohols from a lignocellulosic feedstock. The preferred sugar alcohol is xylitol.

Abstract: The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate. The invention also provides methods to transform normal primary cells so cultured into “cancer stem cells.” The genetically defined cancer stem cell tumor model mimics the behavior of the disease closely, e.g., the cells are invasive, hormone responsive and metastatic when injected into mice. The tumor cells express genes that are specific to cancer stem cells identified in patient samples.

Abstract: The invention relates to methods of evaluating the quality of a batch of an herbal composition, the method comprising subjecting a test batch of the herbal composition to one or more biological analysis methods and comparing the results derived from the test batch to the results of a known batch of herbal composition which has a known in vivo effect.

Abstract: Structures and methods for tissue engineering include a multicellular body including a plurality of living cells. A plurality of multicellular bodies can be arranged in a pattern and allowed to fuse to form an engineered tissue. The arrangement can include filler bodies including a biocompatible material that resists migration and ingrowth of cells from the multicellular bodies and that is resistant to adherence of cells to it. Three-dimensional constructs can be assembled by printing or otherwise stacking the multicellular bodies and filler bodies such that there is direct contact between adjoining multicellular bodies, suitably along a contact area that has a substantial length. The direct contact between the multicellular bodies promotes efficient and reliable fusion. The increased contact area between adjoining multicellular bodies also promotes efficient and reliable fusion.

Abstract: The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

Abstract: Disclosed herein are methods for generating satellite cells and compositions including satellite cells.

Abstract: The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.

Abstract: The disclosure relates to a mixture of bacteria belonging to at least six or seven different and specific bacterial species preferably for use in preventing or treating gastro-intestinal disorders. Preferably, the mixture of bacteria is grown together in a fermenter prior to administering the mixture to a subject in order to prevent or treat the disorder.