Patents Examined by Sheridan Swope
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Patent number: 11078508Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.Type: GrantFiled: December 6, 2018Date of Patent: August 3, 2021Assignee: Novozymes, Inc.Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
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Patent number: 11078509Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.Type: GrantFiled: August 18, 2020Date of Patent: August 3, 2021Assignee: Novozymes, Inc.Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
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Patent number: 11078510Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.Type: GrantFiled: August 18, 2020Date of Patent: August 3, 2021Assignee: Novozymes, Inc.Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
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Patent number: 11066657Abstract: Methods for producing a trans-splicing intein-modified protease with enhanced solubility and regulating its activity are described. Intein-modified proteases having enhanced solubility and polynucleotides encoding the same are provided. Methods of storing trans-splicing proteases are also described.Type: GrantFiled: June 11, 2020Date of Patent: July 20, 2021Assignee: AGRIVIDA, INCInventors: R. Michael Raab, Binzhang Shen, Gabor Lazar
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Patent number: 11053300Abstract: The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.Type: GrantFiled: June 18, 2019Date of Patent: July 6, 2021Assignees: VIB VZW, Universiteit GentInventors: Jan Tavernier, Samuel Lievens
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Patent number: 11015183Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.Type: GrantFiled: December 16, 2019Date of Patent: May 25, 2021Assignee: NOVOZYMES A/SInventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
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Patent number: 11015176Abstract: The present invention provides a luminescent enzyme protein comprising the amino acid sequence represented by SEQ ID NO: 2, and a mutant enzyme thereof.Type: GrantFiled: March 9, 2017Date of Patent: May 25, 2021Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Yoshihiro Ohmiya, Yasuo Mitani, Rie Yasuno
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Patent number: 11008559Abstract: The present disclosure relates to serine proteases cloned from Bacillus Akibai and Bacillus Clarkii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.Type: GrantFiled: May 21, 2018Date of Patent: May 18, 2021Assignee: DANISCO US INCInventors: Marc Kolkman, Rie Mejldal, Anja Hemmingsen Kellett Smith, Lilia Maria Babe, Richard R. Bott
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Patent number: 11001869Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.Type: GrantFiled: August 18, 2020Date of Patent: May 11, 2021Assignee: Novozymes, Inc.Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
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Patent number: 10982232Abstract: Diterpene synthases and methods of their use are described herein.Type: GrantFiled: July 25, 2017Date of Patent: April 20, 2021Assignee: The Regents of the University of CaliforniaInventors: Philipp Zerbe, Sibongile Mafu
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Patent number: 10982205Abstract: The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a Verona integron-encoded metallo-?-lactamase (VIM-2) variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell.Type: GrantFiled: February 22, 2017Date of Patent: April 20, 2021Assignees: DA VOLTERRA, BIOASTERInventors: Jean De Gunzburg, Jean-Denis Docquier
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Patent number: 10975335Abstract: Proteases that comprise an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length and that comprise, in the count in accordance with SEQ ID NO. 1, the amino acid substitution R99D in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I, display very good cleaning performance in particular on blood-containing stains, as well as very good temperature stability.Type: GrantFiled: October 22, 2016Date of Patent: April 13, 2021Assignee: Henkel AG & Co. KGaAInventors: Hendrik Hellmuth, Marion Merkel, Brian Laufs, Susanne Wieland, Timothy O'Connell, Susanne Tondera, Thomas Weber
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Patent number: 10975408Abstract: The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex.Type: GrantFiled: February 19, 2018Date of Patent: April 13, 2021Assignees: OXFORD UNIVERSITY INNOVATION LIMITED, HUMBOLDT-UNIVERSITAT ZU BERLINInventors: Kylie Vincent, Lars Lauterbach, Oliver Lenz
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Patent number: 10941371Abstract: The present disclosure relates to proteases that are variants of a Bacillus gibsonii protease, the proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence given in SEQ ID No. 1 over its entire length, and which has an amino acid substitution on at least one of the positions corresponding to the positions 12, 43, 122, 127, 154, 156, 160, 211 or 222, relating in each case to the numbering according to SEQ ID No. 1. The present disclosure also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.Type: GrantFiled: June 1, 2017Date of Patent: March 9, 2021Assignee: Henkel AG & Co. KGaAInventors: Daniela Herbst, Timothy O'Connell, Nina Mussmann, Inga Kerstin Vockenroth, Thomas Weber
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Patent number: 10935546Abstract: The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.Type: GrantFiled: June 18, 2015Date of Patent: March 2, 2021Assignees: Universite Grenoble Alpes, European Molecular Biology LaboratoryInventors: Imre Berger, Martin Pelosse, Uwe Schlattner
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Patent number: 10927361Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: July 14, 2017Date of Patent: February 23, 2021Assignee: NOVOZYMES A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben P. Friis, Jens E. Nielsen, Roland Alexander Pache
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Patent number: 10927364Abstract: The present invention relates to polypeptides having carbonic anhydrase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: July 20, 2017Date of Patent: February 23, 2021Assignee: Novozymes A/SInventors: Sonja Salmon, Martin Simon Borchert, Thomas Holberg Blicher, Wolfgang Streit, Mirjam Perner
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Patent number: 10928381Abstract: Provided herein are compositions and systems comprising engineered cells useful for the measurement of autophagy induction, maturation and flux in a high-throughput manner. In particular, provided herein are human embryonic kidney (HEK) cells that express the native autophagy substrate and marker protein LC3 fused to Dendra2, a photoconvertable fluorescent protein; this manipulation allows assessment of all three stages of autophagy (e.g., induction, maturation, and cargo degradation), noninvasively in living cells, without the need for protein overexpression that may interfere with autophagy activity.Type: GrantFiled: February 28, 2019Date of Patent: February 23, 2021Assignee: The Regents of the University of MichiganInventors: Sami Barmada, Nathaniel Safren, Elizabeth Tank
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Patent number: 10920209Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.Type: GrantFiled: February 13, 2020Date of Patent: February 16, 2021Assignee: NOVOZYMES A/SInventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
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Patent number: 10920253Abstract: Described is a method for the enzymatic production of acetyl phosphate from formaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.Type: GrantFiled: March 13, 2015Date of Patent: February 16, 2021Assignee: Scientist of Fortune, S.A.Inventor: Philippe Marliere