Abstract: A programmable probe design of DNA micro array and detection methodology is provided. DNA probes, which are complemented with the target DNA, are designed and classified into groups according to optimum hybridization temperature. The probes are arrayed by the group and immobilized on the substrate surface of the DNA micro array. The control system, imaging system and temperature control system are programmed to cooperate with each other during the detection process. This design increases the detection capabilities of the parallel-analysis system.
Abstract: The invention relates to compositions, kits, and methods for detecting, characterizing, preventing, and treating human pancreatic adenocarcinoma. A variety of chromosomal regions (MCRs) and markers in the MCRs, are provided that are correlated with cancer. In particular, chromosomal regions and markers in the MCR 50.06-62.89 Mb of human chromosome 19, are provided, wherein alterations in the copy number of the MCR and/or alterations in the amount, structure, and/or activity of one or more of the markers in the MCR is correlated with the presence of pancreatic adenocarcinoma.
Type:
Grant
Filed:
May 27, 2005
Date of Patent:
April 7, 2015
Assignee:
Dana-Farber Cancer Institute, Inc.
Inventors:
Lynda Chin, Cameron W. Brennan, Ronald A. DePinho, Andrew J. Aguirre
Abstract: Compositions and reaction mixtures are provided for the detection of small RNA target nucleic acids, preferably miRNA target nucleic acids, wherein the compositions and reaction mixtures provide for sensitive and specific detection of the target nucleic acids. The compositions and reaction mixtures include one or more of a first amplification oligomer that is preferably an extender primer, a target capture oligomer that is preferably at least partially double stranded, a promoter primer/provider, a reverse primer that is preferably a universal primer and a detection probe. The compositions and reaction mixtures are useful for diagnostics, prognostics, monitoring the effectiveness of treatment and/or determining a treatment.
Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyzes include nucleic acid amplification and detection and immuno-PCR.
Type:
Grant
Filed:
February 12, 2013
Date of Patent:
January 27, 2015
Assignee:
BioFire Diagnostics, LLC
Inventors:
Kirk M. Ririe, Michael R. Newswander, Randy P. Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E. Jones, Gary Clark Kessler
Abstract: A method of determining a probability that a human test subject has colorectal cancer as opposed to not having colorectal cancer is disclosed.
Abstract: The invention provides a yeast-enhanced red fluorescent protein. In an embodiment of the invention, the yeast-enhanced red fluorescent protein is monomeric and is expressible in Candida albicans. The invention also provides a novel visible color marker for plasmid expression in yeast, particularly Saccharomyces cerevisiae and Candida albicans.
Type:
Grant
Filed:
February 26, 2009
Date of Patent:
December 30, 2014
Assignee:
The Research Foundation for The State University of New York
Abstract: The present invention provides a microfluidic biochip, which comprises: a fluid transportation unit having a fluid transportation reservoir and a fluid transportation air chamber; a first fluid storage reservoir; a second fluid storage reservoir; a first valve unit having a first valve and a first valve control air chamber; and a second valve unit having a second valve and a second valve control air chamber; wherein the first valve unit is located between the first fluid storage reservoir and the fluid transportation unit, the second valve unit is located between the second fluid storage reservoir and the fluid transportation unit, and the top portion of the fluid transportation reservoir and the valves are made of a flexible material. The structure of the present microfluidic biochip allows fluids to be transported and/or mixed therein.
Abstract: Systems and methods are provided for single-molecule sequencing of template nucleic acids in which the signal from a label attached to a polymerase enzyme is used to monitor conformational changes in the polymerase which occur while labeled nucleotides or nucleotide analogs are added to a growing nucleic acid chain which is complementary to the template nucleic acid. The signal indicative of the conformational state of the enzyme is used to determine with higher confidence when true nucleotide or nucleotide analog incorporation events occur, allowing for the improved quality of base calls and sequence determination.
Type:
Grant
Filed:
December 14, 2010
Date of Patent:
December 16, 2014
Assignee:
Pacific Biosciences of California, Inc.
Inventors:
Mark Chaisson, Sonya Clark, Christopher Silk, John Lyle, Lei Jia, Keith Bjornson
Abstract: A non-contact system for sorting monodisperse water-in-oil emulsion droplets in a microfluidic device based on the droplet's contents and their interaction with an applied electromagnetic field or by identification and sorting.
Type:
Grant
Filed:
January 27, 2011
Date of Patent:
July 1, 2014
Assignees:
Lawrence Livermore National Security, LLC, The Regents of the University of California
Inventors:
Neil Reginald Beer, Abraham Lee, Andrew Hatch
Abstract: This invention is related to PNA probes, probe sets, mixtures, methods and kits pertaining to the determination of Mycoplasma and related Mollicutes.
Type:
Grant
Filed:
February 20, 2012
Date of Patent:
June 17, 2014
Assignee:
Applied Biosystems, LLC
Inventors:
Jens Hyldig-Nielsen, Susan Rigby, Michael Tanner, Ditte Lee, Byron Brehm-Stecher
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Type:
Grant
Filed:
February 15, 2012
Date of Patent:
May 20, 2014
Assignee:
Cepheid
Inventors:
Jesus Ching, Ronald Chang, Douglas Dority, Jian Ping Zhang, James Jian Quan Wang, Wendy Wong, Kendra Lara Paul, Reuel Van Atta
Abstract: The present invention provides methods of determining the size of a particular nucleic acid segment of interest in a sample of nucleic acids through fragmentation of DNA, size fractionation, an optional second fragmentation, and identification using a marker sequence. In particular aspects, an expansion or reduction of tandem repeat sequences can be detected. In further aspects, carriers and individuals afflicted with fragile X syndrome or other diseases associated with tandem repeats can be distinguished from normal individuals.
Type:
Grant
Filed:
April 11, 2007
Date of Patent:
April 15, 2014
Assignees:
Quest Diagnostics Investments Incorporated, US Genomics, Inc.
Inventors:
Donghui Huang, Charles M. Strom, Steven J. Potts, Jenny Ellen Rooke
Abstract: Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.
Abstract: Methods are described for determining the pattern of cytosine methylation in a DNA specimen, where the methods involve comparing the amount of DNA fragments generated by a methylation-sensitive restriction enzyme with the amount of DNA fragments generated by a methylation-insensitive isoschizomer of the methylation-sensitive restriction enzyme.
Type:
Grant
Filed:
May 19, 2008
Date of Patent:
February 4, 2014
Assignees:
Albert Einstein College of Medicine of Yeshiva University, The Research Foundation State University of New York
Abstract: The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5?-activated, 3?-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.
Type:
Grant
Filed:
November 3, 2010
Date of Patent:
November 5, 2013
Assignee:
Epicentre Technologies Corporation
Inventors:
Ramesh Vaidyanathan, Scott Kuersten, Ken Doyle
Abstract: Harvesting the full richness of biodiversity is instantly recognized by Diversa Corporation as a powerful means to access both novel molecules having direct commercial utility as well as molecular templates that could be retooled to acquire commercial utility. A directed evolution process for rapid and facilitated production from a progenitor polynucleotide template, of a library of mutagenized progeny polynucleotides wherein each of the 20 naturally encoded amino acids is encoded at each original codon position. This method, termed site-saturation mutagenesis, or simply saturation mutagenesis, is preferably based on the use of the degenerate N,N,G/T sequence. Also, a method of non-stochastically producing a library of chimeric nucleic acid molecules having an overall assembly order that is chosen by design. Accordingly, a set of progenitor templates, such as genes (e.g. a family of esterase genes) or genes pathways (e.g.