Abstract: A novel soybean cultivar, designated 89248009206, is disclosed. The invention relates to the seeds of soybean cultivar 89248009206, to the plants of soybean 89248009206 and to methods for producing a soybean plant produced by crossing the cultivar 89248009206 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 89248009206 with another soybean cultivar.

Abstract: A method of transforming and regenerating soybean plants relies on selection of hypocotyl explants as the target material. Hypocotyl explants can be transformed either by microparticle bombardment with DNA-coated microparticles of inert metals, or by co-culturing with an Agrobacterium strain. The transformed explants can be successfully regenerated, using a protocol including culturing on a shoot induction medium, followed by transfer to a shoot elongation medium to form rooted plantlets, which are transplanted to soil.

Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.

Abstract: The invention provides genetic suppressor elements that confer upon a cell resistance to platinum-based drugs, including cisplatin, methods for identifying and obtaining such elements, and methods of using such elements. The invention also provides cloned genes associated with sensitivity to cisplatin.

Abstract: A process for the preparation of an antiviral plant, which comprises transforming a plant with an expression vector containing a lactoferrin (Lf) gene and culturing the transformed plant.

Abstract: This invention relates to the identification of a gene involved in the gibberellin signal transduction pathway. Mutations to this gene mimic the effect of gibberellin treatment and transgenic plants expressing the gene correct a spindly phenotype. Methods are disclosed for isolating and using the gene from a variety of plants.

Abstract: Salvia is regenerated via organogenesis using plant tissue culture techniques in a multistage culturing process. Roots can be induced from regenerated shoots, and the regenerated plants can be transferred to soil for further growth after the root system is well established.

Abstract: The present invention provides nucleic acid sequences from ovule-specific genes. The nucleic acids are useful in targeting gene expression to ovules or in modulating ovule development.

Abstract: A novel soybean cultivar, designated 9235669440005, is disclosed. The invention relates to the seeds of soybean cultivar 9235669440005, to the plants of soybean 9235669440005 and to methods for producing a soybean plant produced by crossing the cultivar 9235669440005 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 9235669440005 with another soybean cultivar.

Abstract: This invention provides viral and retroviral vectors which comprises a nucleic acid molecule encoding a human cytosolic aldehyde dehydrogenase or a glutamylcysteine synthetase or combinations thereof. Further, this invention provides an isolated mammalian nucleic acid molecule encoding a cytosolic aldehyde dehydrogenase and glutamylecysteine synthetase. In addition, this invention provides a method for reducing the toxic effects of a cyclophosphamide in a subject which comprises replacing the subject's hematopoietic cells with hematopoietic cells having the retroviral vector. Further, this invention provides a method for introducing a selectable marker into a mammalian cell which comprises transfecting the cell with a nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase or glutamylcysteine synthetase.

Abstract: The purified and characterization of hypoxiainducible factor 1 (HIF-1) is described. HIF-1 is composed of subunits HIF-1.alpha. and HIF-1.beta.. Purified HIF-1.alpha. polypeptide, its amino acid sequence and polynucleotide sequence are provided. A HIF-1.alpha. variant that dimerizes to HIF-1.beta. producing a nonfunctional HIF-1 complex is described. Methods for the prevention and treatment of hypoxia-related disorders are provided.

Abstract: Amino acid and nucleotide sequences relating to the glutamate dehydrogenase (GDH) enzyme are described. The GDH enzymes described herein were discovered in the alga Chlorella sorokiniana in the form of seven different inducible isoenzymes. These isoenzymes are found in the algae as chloroplast-localized hexamers composed of .alpha.- and .beta.-subunits. Plants transformed with nucleotide sequences encoding the .alpha.- or .beta.-subunits of the enzyme show improved properties, for example, increased growth and improved stress tolerance.

Abstract: A cloned DNA encoding phosphoenolpyruvate carboxykinase of a C.sub.4 plant is disclosed. The DNA according to the present invention encodes the amino acid sequence shown in SEQ ID NOS: 1-6 in Sequence Listing or the same amino acid sequence as shown in SEQ ID NOS: 1-6 except that one or more amino acid is added, deleted, inserted or substituted, with the proviso that the polypeptide having this amino acid sequence has phosphoenolpyruvate carboxykinase activity.

Abstract: DNA constructs are provided for stable transformation of plastids of multicellular plants and expression of foreign proteins in plastids. The DNA constructs comprise a transforming DNA which is targeted to a pre-determined location on the plastid genome and inserted into the plastid genome by homologous recombination with targeting segments comprising DNA sequences homologous to the pre-determined region of the plastid genome. The transforming DNA contains a non-lethal selectable marker gene which confers a selectable phenotype on cells having plastids in which substantially all of the genomes therein contain the transforming DNA (i.e., homoplasmic cells or tissues). The transforming DNA further comprises at least one insertion site 4 for an additional DNA segment, such as a gene encoding a protein for improving a characteristic of the transformed plant.

Abstract: Broadly this invention provides inbred corn line ZS01220. The methods for producing a corn plant by crossing the inbred line ZS01220 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01220 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01220 with at least one other corn line.

Abstract: The present invention provide nucleic acids encoding polypeptides which confer resistance to Xanthomonas spp. The nucleic acids can be used to produce transgenic plants resistant to the pathogen.

Abstract: A method for inhibiting the growth and/or germination of a fungus that is susceptible to an osmotin protein by contacting the fungus, or causing the fungus to be contacted with, an effective amount of an osmotin protein. The fungus can be, for example, a plant pathogenic fungus, such as an Oomycete. In one embodiment of the invention, the effective amount of the osmotin protein is produced by a plant having incorporated into its genome a chimeric gene having a) an open reading frame encoding the osmotin protein, or a precursor of the osmotin protein, and having operably linked thereto b) a regulatory region which causes the chimeric gene to be expressed in the plant.

Abstract: The novel soybean cultivar, designated CX205, is disclosed. The invention relates to the seeds of soybean cultivar CX205, to the plants of soybean CX205 and to methods for producing a soybean plant produced by crossing the cultivar CX205 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar CX205 with another soybean cultivar.

Abstract: A method for the expression of multiple proteins in a transgenic plant comprising inserting into the genome of the plant a gene construct comprising a 5'-region which includes a promoter which is capable of initiating transcription of a structural gene under the control thereof, a protein encoding sequence coding for more than one protein and a 3'-terminator region which includes a polyadenylation signal, each of the protein encoding sequences being separated from an adjacent protein encoding sequence by a DNA sequence which on translation provides a cleavage site whereby the expressed polyprotein is post-translationally processed into the component protein molecules. The DNA sequence which encodes the post-translation cleavage site may be derived from a virus, particularly a picornavirus. In a preferred embodiment the DNA sequence providing the cleavage site encodes the amino acid sequence NFDLLKLAGDVESNPGPFF.

Abstract: The present invention relates to NF-X1, a novel DNA binding protein which regulates expression of major histocompatibility complex (MHC) class II molecules, and to DNA sequences which encode the protein as well as recombinant expression of the protein. NF-X1 is a newly identified, cysteine-rich polypeptide which interacts sequence-specifically with the conserved X1 box regulatory element found in the proximal promoters of class II MHC genes. A cysteine-rich domain within NF-X1 contains a motif repeated seven times, and this entire region is necessary and sufficient for both sequence specific binding and effector function. The motif is related to but distinct from the previously described metal-binding protein families: LIM domain and RING finger. NFX.1 mRNA is markedly overexpressed late after induction of cells with interferon-gamma, and this overexpression coincides with a reduction in the level of HLA-DRA transcript in these cells.