Abstract: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
Type:
Grant
Filed:
August 28, 2008
Date of Patent:
April 10, 2012
Assignee:
Medtronic, Inc.
Inventors:
Barton Holmquist, Daniel J. Strydom, Jin Seog Seo
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