Abstract: Among other things, the present invention is related to bio/chemical sampling, sensing, assays and applications.

Abstract: The present invention relates to a multiparameter lateral flow strip (1), comprising a microporous membrane layer (2) supported on a liquid-impermeable support layer (3), for lateral flow of a liquid through the microporous membrane layer (2), wherein the microporous membrane layer (2) has two or more flow lanes (4) in the direction of lateral flow, wherein said two or more flow lanes (4) are separated by hydrophobic separation channels (5), and wherein each of said two or more flow lanes (4) comprises a detection spot (6) including a binding agent, wherein said two or more flow lanes (4) are isomorphic lanes. Furthermore, the present invention relates to a multiparameter lateral flow immunoassay device comprising said multiparameter lateral flow membrane (1), the use of said multiparameter configured lateral flow membrane (1) in an immunological test, as well as to a method for the manufacture of said multiparameter configured lateral flow membrane (1).

Abstract: The present disclosure relates to an intercellular proximity labeling technique. According to an embodiment of the present disclosure, intercellular proximities can be distinguished by cell types, in particular, synapses of nerve cells can be distinguished by types.

Abstract: Disclosed is a calibrator comprising IgA having a biotin group and a biotin-binding site, the calibrator being used to obtain a concentration of an IgA aggregate in a sample.

Abstract: A method of detecting a target biological entity in a biofluid using a sensor, wherein the biofluid comprises a plurality of the target biological entities and nanoparticles, the sensor comprising a substrate bearing a pair of electrodes having an affinity with the nanoparticles, and wherein a region between the electrodes defines a sensing region.

Abstract: A chemigenetic calcium indicator and a method of measuring calcium are provided. The chemigenetic calcium indicator includes a calcium-binding protein domain attached to a ligand binding protein domain. The method of measuring calcium includes administering a chemigenetic calcium indicator to a subject and determining changes in fluorescence, the chemigenetic calcium indicator including a ligand binding protein domain having a calcium-binding protein domain and a dye-ligand conjugate attached thereto.

Abstract: A glycemic management related analyte detecting assay device (10) and method are provided for detecting and quantifying analyte concentrations in a fluid sample. The assay device includes an absorbent body containing an assay forming a detection zone for receiving a fluid test sample. The absorbent body is provided in a chamber of the device. The assay can detect one or more of a glycemic analyte selected from the group consisting of fasting plasma blood glucose, oral glucose, % glycated hemoglobin, and fasting insulin concentrations. In one embodiment, a container includes an absorbent body having a plurality of superimposed membranes (30, 32, 34, 36) where each membrane contains a reactant and a color indicator for detecting the presence of a selected analyte above a predetermined concentration in the fluid sample. The absorbent body and/or the assay include a color indicator that is able to provide a visual indication of the presence of one or more glycemic analytes present in the test sample.

Abstract: Match-paired monoclonal antibodies against major royal jelly protein 4 (MRJP4) are secreted by hybridoma cell lines having microbial deposit numbers of CGMCC No. 17294 and CGMCC No. 17295, which are used in an ELISA kit and a colloidal gold immunoassay strip for detecting the MRJP4. The positive and MRJP4-specific cell lines are obtained by cell fusion using an antigen of MRJP4 recombinant protein and a cross-reaction with other major royal jelly proteins. The MRJP4 recombinant protein is used as an antigen to obtain several positive cell lines by cell fusion and two MRJP4-specific fusion cell lines are obtained by a cross-reaction with other major royal jelly proteins. Primary screening of a matched antibody pair is performed according to antibody pairing for recognizing different epitopes.

Abstract: The pocket detection platform (PDP) detects pathogens, toxins and chemicals of interest simultaneously by way of a multi-channeled, soft see-through plastic pouch design that consists of inner and outer compartments that promote compartmentalization and/or unidirectional sample flow. The platform enables the concurrent running of multiple detection assay techniques such as lateral flow immunoassays (LFI), Isothermal molecular assays (i.e., Recombinase Polymerase Amplification, or RPA) and/or paper-based chemical assays (i.e., M8, pH paper) from a single wet or dry sample with minimal sample processing. The PDP reduces soldier overburden by decreasing size weight, and power (SWaP) as well as training time, electronic burden, while providing a flexible, customizable assay platform that can be rapidly produced, assembled, sustained, and when contaminated, easily to dispose of.

Abstract: A time-resolved fluorescence immunochromatography test paper card for detecting butralin, which comprises a cover body and a housing body, wherein the cover body is provided with a test hole, a loading hole and a through-hole, an isolating mechanism is arranged in the test hole and the loading hole, the isolating mechanism comprises a first isolating ring and a second isolating ring, the top lateral walls of which are respectively provided with a first lug boss and a second lug boss, one end of the upper surface of the working board is concave towards the inner of the working board to provide a groove, there is a nitrocellulose membrane, a binding pad, a sample pad and a mark zone successively provided between the water absorbing block and the other end of the working board, and the lateral wall at one end of the working board is provided with a bump.

Abstract: Aspects of the disclosure relate to lateral flow assays having a number of different detection zones tuned to extend the dynamic range of the assay in indicating concentration of a hazardous contaminant in a test sample. Some aspects relate to assay reader devices configured with instructions to generate a result representing the concentration of the hazardous contaminant in the test sample based on some or all of the detection zones.

Abstract: Provided are: a coloring cellulose microparticle enabling false positive to be significantly reduced while maintaining a high detection sensitivity; and an immunochromatographic diagnostic kit using the same.

Abstract: The present invention relates to a test system or an assay system (detection system) and test method preferably for use in the Point-of-Care (PoC) field.

Abstract: The disclosure concerns a method for determining the interaction between a test compound and a receptor. The receptor may be immobilized. The disclosure also concerns a sample holder assembly including a functionalized test well wall, which may be used in combination with a Total Internal Reflection Fluorescence source.

Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.

Abstract: A spectral reflectance imaging device for detecting nanoparticle exosome biomarker targets includes an illumination source that illuminates a substrate with a plurality of separate wavelengths of incoherent light. The substrate includes an oxide layer and a binding agent to selectively bind nanoparticle exosome biomarker targets to the substrate. An imaging device bindings the light reflected from or transmitted through the substrate and an image processing system detects the nanoparticle exosome biomarker targets a function of the change in reflective properties of the substrate.

Abstract: The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.

Abstract: Antibodies are used as biomarkers to assist in distinguishing gluten immune reactivity and sensitivity, silent celiac disease, Crohn's disease and other gut-related pathologies from classical celiac disease. In one class of embodiments, sera, saliva or other samples from a human or other animal are tested for antibodies to (a) a wheat antigen; (b) a gliadin antigen; and (c) one or more of a wheat germ agglutinin, a gluteomorphin, a glutenin, a deamidated glutenin, a prodynorphin, and a dynorphin. Test results are considered particularly interesting where the wheat antigen and the gliadin antigen are both selected from the group consisting of native and deamidated forms of ?-gliadin 33-mer, ?-gliadin-17-mer, ?-gliadin-15-mer, ?-gliadin-17-mer, and glutenin 21-mer.

Abstract: The present disclosure relates to methods for determining a wavefront position of a liquid on a surface of an assay test strip placing a liquid on the surface of the test strip; and acquiring one or more signals from the surface of the test strip at one or more times, comparing the one or more acquired signals to a threshold, wherein the wavefront position is a position on the surface of the test strip where a signal is greater than or less than a threshold (e.g., fixed or dynamic threshold). Such methods may be used to determine the wavefront velocity of a liquid on a surface of an assay test strip and the transit time of a liquid sample to traverse the one or more positions on the surface of the assay test strip.

Abstract: Aspects of the present disclosure relate to binding agents (e.g., antibodies and antigen binding fragments) that bind soluble fms-like tyrosine kinase 1 (sFlt1) in an isoform specific manner. In some embodiments, immunological assay methods utilizing isoform-specific antibodies or antigen binding fragments that bind sFlt1 isoforms are provided for assessing biological samples obtained from pregnant subjects, e.g., for purposes of evaluating preeclampsia status in the subject.