Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

Abstract: Antifoams based on oil-in-water emulsions, in which the oil phase accounts for from 5 to 50% by weight of the emulsions and consists essentially of a mixture of(a) fatty esters of C.sub.12 -C.sub.22 -carboxylic acids with monohydric to trihydric C.sub.1 -C.sub.22 -alcohols,(b) polyglyceryl esters which are obtainable by at least 20% esterification of polyglycerols which contain at least 2 glyceryl units with at least one C.sub.12 -C.sub.36 -fatty acid and(c) fatty esters of C.sub.12 -C.sub.22 -carboxylic acids and polyalkylene glycols, the molecular weight of the polyalkylene glycols being up to 5,000 g/mol, and, if required,(d) long-chain alcohols, fatty esters of alcohols of at least 22 carbon atoms and C.sub.1 -C.sub.36 -carboxylic acids, distillation residues which are obtainable in the preparation of alcohols having a relatively large number of carbon atoms by oxo synthesis or by the Ziegler process and which may be alkoxylated, and/or(e) hydrocarbons having a boiling point above 200.degree. C.

Abstract: A process capable of being continuously carried out, for disintegrating cell material in the form of dispersions or suspensions in water for the purpose of obtaining cell constituent. Selected parameters permit avoidance of use of solid ultrasonication activators and the establishment of a particular geometry form for the acoustic irradiation container. The parameters include sonotrode immersion angle, length of immersion, ratio of extent of immersion of the sonotrode relative to the acoustic irradiation volume and the ratio of extent of immersion to the solid matter content of the medium to be sonicated.

Abstract: A process for maintaining a high rate of mass transfer of nutrients contained in a liquid feed stream to a biofilm of microorganisms supported on a bed of particulate matter. The microorganisms in the biofilm are subjected to a continuous, uniform flow of the liquid feed steam and a countercurrent flow of respiratory air so as to support biofilm growth. The bed is periodically pulsed with air at a pressure sufficient to shed microorganisms from the biofilm, and at intervals sufficient to avoid preferential channeling of the liquid feed steam through the bed and/or to disrupt and rearrange the bed of particulate matter.

Abstract: A centrifugal rotor effects the isolation, in a sequence of steps, of a substance from a mixture of substances dissolved, suspended or dispersed in a sample liquid. Multiple samples are processed simultaneously by means of a plurality of fractionation cells, each of which contains a series of interconnected, chambered and vented compartments in which individual steps of the fractionation and isolation procedure take place. Specific steps in the preferred embodiments include lysis, sedimentation, aggregation, sorption, rinsing, and desorption. The specific compartment occupied by the sample liquid or one of its fractions at any stage of the process is governed by the speed and direction of rotation of the rotor and by gravitational force. The interconnections, chambers and passages of each compartment are sized and angled to prevent predetermined amounts of sample and reagent liquids from overflowing the compartment.

Abstract: A cover effective to releasably seal a multiwell container, such as a microtitration plate, is disclosed. The cover contains a pad, fashioned from a flexible polymer sheet, and a plurality of resiliently compressible ridges formed on the sheet. The ridges are deformable, such that application of pressure applied to the cover is effective to form a fluid-tight seal between the pad and the well openings. The ridges extend from the pad sufficiently to break the seal upon release of the pressure.

Abstract: The invention provides a compact device and simple method for circulating fluid over the surface of a membrane. The device comprises a housing that in combination with the surface of the membrane defines an enclosed volume through which the fluid is circulated. Some embodiments will include a membrane holding layer, a fluid dispersing layer, or both. The membrane holding layer has an opening through it to expose a predetermined surface area of the membrane to the fluid. The dispersing layer has a set of openings, typically slits, through the dispersing layer to disperse fluid over the surface of the membrane. Some embodiments may include electrodes for sensing or applying electrical potential to the membrane. A particularly preferred embodiment comprises a double sided device including a first housing, dispersing layer and membrane holding layer on one side of a membrane and a second housing dispersing layer and membrane holding layer on the other side of the membrane.

Abstract: A filter device containing cell masses and single cells is described. The device contains porous hollow fibers and hepatocytes entrapped within a contracted gel matrix.

Abstract: An improved apparatus for conducting gas-liquid reactions, comprising a vessel having at least one ascending flow chamber and at least one descending flow chamber. The vessel is especially useful in conducting high cell density fermentation processes, for maintaining a predetermined and relatively high gas holdup within the vessel and simultaneously preventing foaming through hydrodynamics.

Abstract: A method for making uncreped throughdried tissues is disclosed in which the dried tissue sheet is fully supported by a fabric up to the reel. This method eliminates the open draw between the throughdryer and the reel and thereby eliminates sheet breaks normally associated with such open draws. In addition, the machine direction strength of the sheet can be reduced since the added strength is not needed to traverse the open draw normally present in current processes. Reducing the MD strength in turn enables the production of more square, less stiff sheet, which improves the tactile properties of the product.

Abstract: A method for making uncreped throughdried tissues is disclosed in which the dried tissue sheet is fully supported by a fabric up to the reel. This method eliminates the open draw between the throughdryer and the reel and thereby eliminates sheet breaks normally associated with such open draws. In addition, the machine direction strength of the sheet can be reduced since the added strength is not needed to traverse the open draw normally present in current processes. Reducing the MD strength in turn enables the production of more square, less stiff sheet, which improves the tactile properties of the product.

Abstract: The invention provides a compact device of simple construction for holding a membrane in contact with a pair of fluids, one on each side of the membrane. According to the invention, the membrane holder comprises a base and an upper cap, with the membrane held between them. Fluids may be circulated over the surfaces of the membrane. Electrode ports are provided through which electrodes may be placed in proximity to the membrane for measuring or applying electrical potential across the membrane. The membrane holder comprises a base, which includes a lower plate and a center plate secured thereto, and an upper cap, which screws into the base by means of a threaded connection between them. The upper cap may be sealed to provide an enclosed volume for holding one of the fluids or the upper cap may be open to provide an open reservoir for holding the fluid. One or more membrane holders according to the invention may be placed in and held by a specially constructed heater stand.

Abstract: The invention relates to an apparatus for composting organic leavings in small-scale households etc. According to the invention the apparatus includes a rotating drum (1, 2, 6) with an adjustable partition wall and adjustable cover, by means of which the leavings can be properly mixed which is a presupposition for microbe activity in the mass to be composted.

Abstract: An apparatus for incorporating one or more biologically active substances into red blood corpuscles (erythrocytes) by lysis and resealing technique comprises a washing unit, a lysis unit and a resealing unit. The washing unit obtains a suspension of erythrocytes from complete or incomplete blood. The lysis unit cools the erythrocyte suspension to a temperature below 10.degree. C. and maintains this temperature while the suspension is processed through a dialysis unit and subsequently exposed to the active substance. The treated erythrocyte suspension is held in a retardation pouch for a predetermined time and thereafter fed to the resealing unit, which heats the treated suspension to a temperature higher than 20.degree. C. and maintains this temperature while the treated suspension is exposed to the resealing product. The suspension exposed to the resealing product is collected in a collecting pouch for a predetermine period before it is discharged to the washing unit.

Abstract: A bioreactor which and be used with substrates which are difficult to attain sufficient dispersion is made up of a reaction vessel 1 having a reaction chamber 2 surrounded by a water jacket 3 for maintaining a predetermined constant temperature. The reaction chamber 2 is provided in the vicinity of the inner wall surface 2a of the reaction vessel 2 with a plurality of upright screws 6 and 7 in an adjoining relationship to each other, each screw having a screw thread or a helical fin 6b, 7b of reverse turning sense with each other so that the reactor vessel contents are subjected to a convectional flow induced by the rotation of the screws in the reverse sense to each other by a gearing 14, 15. The substrate therein is subjected to a uniform agitation and to a milling and sieving actions in the portion 13 where the fins of the screws face each other, before the reaction product is removed by a screw conveyer 25.

Abstract: An insert for trans-membrane co-culture includes a hollow housing with a passageway therethrough, a first end, a second end and a center section intermediate the first and second end. The passageway has an inside diameter at the first end and the second end that is larger than the inside diameter of the passageway at the center section so that the passageway tapers from each end to the center section. The center section includes a porous membrane having a first surface and a second surface attached across and obstructing the passageway. The membrane forms a first receptacle from the passageway toward the first end and forms a second receptacle from the passageway toward the second end. A method for using the insert of the present invention to grow cells on both sides of a porous membrane includes horizontally submerging the insert in liquid medium so that the air in the receptacles is displaced by the medium.

Abstract: A portable toxicity tester for use in the field using bioluminescent organisms consisting of a light tight container for housing the necessary flash of light detecting apparatus and the necessary signal processing circuits, digital readout and control panel. A light tight chamber is provided with a removable stirrer assembly which is the stirrer motor housing as well as the light tight cover for the chamber. With the cover removed, a vial containing the necessary inoculated bioluminescence organisms is inserted into a holder in the chamber. Part of the stirrer assembly is a paddle attached to the motor shaft that extends to near the bottom of the vial. The top of the vial holder extends beyond the outer surface of the cover to insure a light tight seal.

Abstract: The method of making a glass diffusion cell which utilizes taking of an inner glass tube and forming an enlarged annular flange at one end of this tube and in the sidewall of this tube forming a refilling port and a sampling port. This inner glass tube is then located within an outer glass tube with the end of this outer glass tube that is located directly adjacent the enlarged annular flange being sealed to the inner glass tube. The sidewall of the outer glass tube is then spot heated which permits a refilling tube to be connected to the refilling port and a sampling tube to be connected to the sampling port establishing a fluid interconnection with the interior of the inner glass tube. Inlet and outlet tubes are connected to the sidewall of the outer glass tube which fluidly connect with the annular chamber located between the inner glass tube and the outer glass tube. The bottom wall of both the inner glass tube and the outer glass tube are then sealed.

Abstract: The method for processing of potatoes is characterized by the fact that the method is carried out in a potato processing plant, which comprises an oil separator, heating equipment and at least one enzyme reactor and which is modified in such a manner that it can also be used for processing of rape. It is not obvious that such modification is sufficiently small for achieving an economically sound method for processing of rape outside the potato season.

Abstract: An apparatus for counting microorganism colonies on at least one disposable microorganism culturing medium having a substantially planar substrate. The apparatus is adapted to interact with a cassette holding a number of similar substrates at once. The apparatus includes an imaging device capable of detecting colonies on the substantially planar substrate, a cassette positioning device for moving the cassette so that the substrates supported within the cassette are moved sequentially into a predetermined position relative to the imaging device, and an ejecting device for ejecting a substantially planar substrate in turn from the cassette when that substrate is in the predetermined position from the cassette and into an imaging position adjacent the imaging device. Suitable cassettes and a method for counting colonies on substrates supported within a cassette are also disclosed.