Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Abstract: Techniques for secure mobile payment are provided. A credit or gift card is registered for additional security, such that a secure identifier or personal identification number (PIN) is required for use of the credit or gift card. Registration is not required or even known by the third-party payment service associated with the card. Subsequently, when a consumer attempts to use the card and before payment instructions are sent to the corresponding third-party payment service, the secure identifier or PIN is requested and verified. If properly verified, the payment instructions are forwarded for processing by the third-party payment service.

Abstract: An electronic calendar and method of controlling an electronic calendar. A calendar image of a calendar for a person is displayed on a display screen on a display device. The calendar image comprises a plurality of day boxes. A summary of information for the person for a day is displayed in a day box corresponding to the day in the plurality of day boxes. A detail view box is displayed overlaid on the calendar image adjacent to the day box in response to a selection of the day box by an operator. Detailed information for the person for the day is displayed in the detail view box. The detailed information may include work shift information and enable swapping work shifts with others.

Abstract: Systems, methods, and devices are described herein for placing secondary content into a break of unknown duration in a stream of primary content. In one aspect the described techniques may include receiving information indicative of a break in streaming of the primary content, such as metadata data that is inserted into the stream of primary content, for example, on a client device. A duration of the stream of the primary content may be obtained. Secondary content may be played, for example during a break in the streaming of the primary content. Upon detecting that the duration of the stream of the primary content has increased to a second duration, for example, in response to the client device receiving additional primary content, the primary content may be streamed.

Abstract: The present invention relates to methods for genotyping microbial host cells that have been subjected to metabolic engineering. The methods provided herein allow detection of genetic edits in the genome of a microbial host cell using PCR-based genome enrichment following appendage of a common priming site. The compositions and methods of the present invention can be used to confirm engineered metabolic diversity as well as ectopic insertions. Kits for performing the methods are also disclosed.

Abstract: Systems, methods, and devices are described herein for placing secondary content into a break of unknown duration in a stream of primary content. In one aspect, the described techniques may include streaming a segment of primary content, for example, by a reduce capability client device, such as a mobile device or tablet. The device may receive information indicative of a break in the streaming of the primary content. The device may play secondary content based on receiving the information indicative of the break. The device may determine when a next segment of the primary content is available, and begin streaming the next segment of the primary content upon detecting that the second segment of the primary content is available.

Abstract: Techniques are described herein for optimizing the presentation of content through obtaining viewership metrics, generating multiple renditions of presentations of content, and selecting optimized renditions of content. One aspect includes associating a request for viewership metrics with a presentation of content, publishing the presentation of content, and receiving information of the viewership metrics associated with the presentation. Another aspect includes copying a presentation of a content item, creating a second presentation of the content item for publishing over one or more channels, and linking the presentation of the content item to the second presentation of the content item, such that a change to one propagates to the other of the presentation of the content item or the second presentation of the content item. In yet another aspect, a content rendition may be selected for presentation based on performance of the rendition out of multiple renditions in an auction.

Abstract: Systems, devices and methods are described herein for segmentation of content, and more specifically for segmentation of content in a content management system. In one aspect, a method may include receiving content associated with speech, text, or closed captioning data. The speech, the text, or the closed captioning data may be analyzed to derive at least one of a topic, subject, or event for at least a portion of the content. The content may be divided into two or more content segments based on the analyzing. At least one of the topic, the subject, or the event may be associated with at least one of the two or more content segments based on the analyzing. At least one of the two or more content segments may then be published such that each of the two or more content segments is individually accessible.

Abstract: Systems, methods, and devices are described herein for placing secondary content into a break of unknown duration in a stream of primary content. In one aspect the described techniques may include receiving information indicative of a break in streaming of the primary content, such as metadata data that is inserted into the stream of primary content, for example, on a client device. A duration of the stream of the primary content may be obtained. Secondary content may be played, for example during a break in the streaming of the primary content. Upon detecting that the duration of the stream of the primary content has increased to a second duration, for example, in response to the client device receiving additional primary content, the primary content may be streamed.

Abstract: Systems, devices, and methods are described herein for providing a graphical user interface for configuring presentations of content and controlling distribution of content, for example, through in conjunction with a management system.

Abstract: Systems, devices and methods are described herein for segmentation of content, and more specifically for segmentation of content in a content management system. In one aspect, a method may include receiving content associated with speech, text, or closed captioning data. The speech, the text, or the closed captioning data may be analyzed to derive at least one of a topic, subject, or event for at least a portion of the content. The content may be divided into two or more content segments based on the analyzing. At least one of the topic, the subject, or the event may be associated with at least one of the two or more content segments based on the analyzing. At least one of the two or more content segments may then be published such that each of the two or more content segments is individually accessible.

Abstract: Techniques are described herein for optimizing the presentation of content through obtaining viewership metrics, generating multiple renditions of presentations of content, and selecting optimized renditions of content. One aspect includes associating a request for viewership metrics with a presentation of content, publishing the presentation of content, and receiving information of the viewership metrics associated with the presentation. Another aspect includes copying a presentation of a content item, creating a second presentation of the content item for publishing over one or more channels, and linking the presentation of the content item to the second presentation of the content item, such that a change to one propagates to the other of the presentation of the content item or the second presentation of the content item. In yet another aspect, a content rendition may be selected for presentation based on performance of the rendition out of multiple renditions in an auction.

Abstract: Systems, devices and methods are described herein for segmentation of content, and more specifically for segmentation of content in a content management system. In one aspect, a method may include receiving content associated with speech, text, or closed captioning data. The speech, the text, or the closed captioning data may be analyzed to derive at least one of a topic, subject, or event for at least a portion of the content. The content may be divided into two or more content segments based on the analyzing. At least one of the topic, the subject, or the event may be associated with at least one of the two or more content segments based on the analyzing. At least one of the two or more content segments may then be published such that each of the two or more content segments is individually accessible.

Abstract: Systems and methods allowing for a customer to initiate a fuel purchase transaction utilizing a mobile device are described. The customer may further be presented with advertisements on his mobile device and/or be provided with the opportunity to purchase additional items for inclusion on his fuel transaction. In some embodiments, the customer may pay for his transaction using mobile wallet information or other information associated with the mobile device. Alternatively, in some embodiments, the customer may pay for his transaction using input means of the fuel pump.

Abstract: A thermal and environmental noise suppressing interface circuit which is configured to operate cold and is configured to perform biasing with suppression of noise currents from room temperature noise voltages and dc coupled rf readout of a superconducting device under test with a single coaxial cable or equivalent conductor pair. The circuit is configured to suppress the propagation of thermal and environmental noises to/from sensors operating at a different temperature from its operating and control equipment while maintaining a single input-output channel, and provides for the placement of a local grounding impedance on an intercept board.

Abstract: Implementations of the present disclosure provide an adapter for use in a processing chamber. In one implementation, the adapter comprises a hollow body having a first end and a second end opposing the first end, a first block and a second block symmetrically disposed within the hollow body about a longitudinal axis of the body, wherein the first block and the second block define a central opening therebetween, and a retention device disposed in contact with the first and second blocks to confine the movement of the first and second blocks with respect to the hollow body. The central opening is sized so that the first block and the second block provide direct contact with a press seal of the lamp.

Abstract: Systems, devices, and methods are described herein for providing a graphical user interface for configuring presentations of content and controlling distribution of content, for example, through in conjunction with a management system.

Abstract: Systems, methods, and devices are described herein for placing secondary content into a break of unknown duration in a stream of primary content. In one aspect, the described techniques may include streaming a segment of primary content, for example, by a reduce capability client device, such as a mobile device or tablet. The device may receive information indicative of a break in the streaming of the primary content. The device may play secondary content based on receiving the information indicative of the break. The device may determine when a next segment of the primary content is available, and begin streaming the next segment of the primary content upon detecting that the second segment of the primary content is available.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.