Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: The present invention relates to a method for preparing an organically functionalized layered double hydroxide which comprises reacting (a) a layered double hydroxide, (b) a triglyceride oil, (c) an enzyme which is capable of catalyzing the hydrolysis of an ester, and (d) water, to produce the organically functionalized layered double hydroxide. The subject invention further reveals a method for preparing an organically functionalized layered double hydroxide as specified in claim 1 which comprises (1) dispersing into an aqueous medium (a) the layered double hydroxide, (b) the triglyceride oil, and (c) the enzyme, (2) allowing the triglyceride oil to hydrolyze into long-chain fatty acids and glycerol to produce a dispersion of the organically functionalized layered double hydroxide; and (3) recovering the organically functionalized layered double hydroxide from the aqueous dispersion.

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: This invention is based upon the discovery that a hydroxylated carboxylic acids which contain at least 17 carbon atoms and alkaline earth metal salts thereof, such as zinc ricinoleate, act effectively as deodorants in asphalt and asphalt containing compositions. The present invention more specifically discloses an asphalt additive composition which is comprised of (1) an asphalt, (2) 0.05 weight percent to about 4 weight percent of a partitioning agent, and (3) at least 0.1 weight percent of a deodorant selected from the group consisting of (a) a hydroxylated carboxylic acid which contains at least 17 carbon atoms and (b) an alkaline earth metal salt of a hydroxylated carboxylic acid which contains at least 17 carbon atoms. In many applications it is beneficial for the asphalt additive composition to further include 0.5 weight percent to about 50 weight percent of a polymer additive.

Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials.

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.