Abstract: The present disclosure relates to a protocol for the regeneration of Elaeis plants from protoplasts and to the genetic manipulation of the protoplasts to introduce or facilitate expression of desired properties and beneficial traits in Elaeis plants.

Abstract: A method for producing transgenic plants, including treating a target tissue using plasmolyzing media (PM) which contains 4% to 10% of sucrose and 100 ?M to 300 ?M of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension using a suitable strain and a suitable transformation vector. A PM containing 4% to 10% sucrose and 100 ?M to 300 ?M AS is treated for a period between 1 to 3 days. Cultivation is performed in a cultivation media in a dark condition at a temperature between 25° C. to 30° C. A non-selection media with an antibiotic is introduced. A selection media containing an active ingredient phosphinothricin (PPT) is introduced in a light condition at a temperature of between 25° C. to 30° C. in a sub culture for a period of between 3 weeks to 1 month. The putative transformant is regenerated and the number of copies of the transgenes is analyzed.

Abstract: The present invention relates to an isolated nucleic acid molecule comprising a sequence of nucleotides encoding a ?-ketoacyl ACP synthase II from a plant, or a variant, derivative, homolog or analog of said ?-ketoacyl ACP synthase II. The invention also relates to an isolated ?-ketoacyl ACP synthase II enzyme from a plant. The polynucleotide sequences of the present invention may be used inter alia for the generation of cells or plants comprising such cells which have a modified phenotype and/or which exhibit a desired characteristic.

Abstract: A method for producing transgenic plants, including treating a target tissue using plasmolying media (PM) which contains 4% to 10% of sucrose and 100 ?M to 300 ?M of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension using a suitable strain and a suitable transformation vector. A PM containing 4% to 10% sucrose and 100 ?M to 300 ?M AS is treated for a period between 1 to 3 days. Cultivation is performed in a cultivation media in a dark condition at a temperature between 25° C. to 30° C. A non-selection media with an antibiotic is introduced. A selection media containing an active ingredient phosphinothricin (PPT) is introduced in a light condition at a temperature of between 25° C. to 30° C. in a sub culture for a period of between 3 weeks to 1 month. The putative transformant is regenerated and the number of copies of the transgenes is analyzed.