Patents by Inventor Achim Karger
Achim Karger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11905557Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.Type: GrantFiled: March 5, 2021Date of Patent: February 20, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Swati Goyal, Achim Karger, Peter Ma, S. Jeffrey Rosner, Ian Walton, Jonathan Wang, Michael Wenz
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Publication number: 20230314369Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.Type: ApplicationFiled: February 10, 2023Publication date: October 5, 2023Inventors: Jen-Kuei LIU, Bharti SOLANKI, Hans Michael WENZ, Frank MERCER, Cevat AKIN, Achim KARGER, Annelise BARRON, Scott MACK
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Patent number: 11754524Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.Type: GrantFiled: July 7, 2020Date of Patent: September 12, 2023Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Ian Walton, Robert Cobene, Alexander Dukhovny, Adam Sannicandro, John Dixon, Achim Karger, Dan Kline
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Patent number: 11619608Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.Type: GrantFiled: May 22, 2018Date of Patent: April 4, 2023Inventors: Jen-Kuei Liu, Bharti Solanki, Hans Michael Wenz, Frank Mercer, Cevat Akin, Achim Karger, Annelise Barron, Scott Mack
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Publication number: 20210269871Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.Type: ApplicationFiled: March 5, 2021Publication date: September 2, 2021Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Swati GOYAL, Achim KARGER, Peter MA, S. Jeffrey ROSNER, Ian WALTON, Jonathan WANG, Michael WENZ
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Publication number: 20200333291Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.Type: ApplicationFiled: July 7, 2020Publication date: October 22, 2020Inventors: Ian WALTON, Robert COBENE, Alexander DUKHOVNY, Adam SANNICANDRO, John DIXON, Achim KARGER, Dan KLINE
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Patent number: 10746695Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.Type: GrantFiled: March 6, 2015Date of Patent: August 18, 2020Assignee: Life Technologies CorporationInventors: Ian Walton, Robert Cobene, Alexander Dukhovny, Adam Sannicandro, John Dixon, Achim Karger, Dan Kline
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Publication number: 20200141901Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.Type: ApplicationFiled: May 22, 2018Publication date: May 7, 2020Inventors: Jen-Kuei LIU, Bharti SOLANKI, Hans Michael WENZ, Frank MERCER, Cevat AKIN, Achim KARGER, Annelise BARRON, Scott MACK
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Patent number: 10274460Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.Type: GrantFiled: March 6, 2015Date of Patent: April 30, 2019Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Ian Walton, Achim Karger, Alexander Khorlin, Michael Simon, Adam Sannicandro, Alexander Dukhovny, Robert Cobene, Dan Kline, John Dixon
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Publication number: 20170176385Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.Type: ApplicationFiled: March 6, 2015Publication date: June 22, 2017Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ian Walton, Achim Karger, Alexander Khorlin, Michael Simon, Adam Sannicandro, Alexander Dukhovny, Robert Cobene, Dan Kline, John Dixon
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Publication number: 20170176384Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.Type: ApplicationFiled: March 6, 2015Publication date: June 22, 2017Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ian Walton, Robert Cobene, Alexander Dukhovny, Adam Sannicandro, John Dixon, Achim Karger, Dan Kline
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Publication number: 20170051344Abstract: According to various embodiments described herein, a microfiuidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfiuidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfiuidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.Type: ApplicationFiled: January 23, 2015Publication date: February 23, 2017Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Swati Goyal, Achim Karger, Peter Ma, S. Jeffrey Rosner, Ian Walton, Jonathan Wang, Michael Wenz
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Publication number: 20140162258Abstract: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.Type: ApplicationFiled: October 21, 2013Publication date: June 12, 2014Applicant: Applied Biosystems, LLCInventors: Achim KARGER, James Rozzelle, Chien-Wei Chang, Elena Bolchakova
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Patent number: 8530158Abstract: Systems and methods for processing a biological sample are provided herein. For example, the system can be configured to deaggregate/declump a sample before, during, and/or after sample preparation and/or sample analysis. For example, the system can include a deaggregation device/system in communication with, for example, a nucleic acid amplification process (e.g., an ePCR system). Various embodiments of the deaggregation device are provided herein. For example, in some embodiments, the deaggregation device can include a valve, a valve manifold, a conduit, a channel, or some combinations thereof.Type: GrantFiled: May 10, 2011Date of Patent: September 10, 2013Assignee: Life Technologies CorporationInventors: Maengseok Song, Achim Karger, Patrick Kinney
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Patent number: 8089623Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: GrantFiled: August 18, 2009Date of Patent: January 3, 2012Assignee: Applied Biosystems LLCInventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Publication number: 20110275125Abstract: Systems and methods for processing a biological sample are provided herein. For example, the system can be configured to deaggregate/declump a sample before, during, and/or after sample preparation and/or sample analysis. For example, the system can include a deaggregation device/system in communication with, for example, a nucleic acid amplification process (e.g., an ePCR system). Various embodiments of the deaggregation device are provided herein. For example, in some embodiments, the deaggregation device can include a valve, a valve manifold, a conduit, a channel, or some combinations thereof.Type: ApplicationFiled: May 10, 2011Publication date: November 10, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Maengseok SONG, Achim Karger, Patrick Kinney
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Publication number: 20110123985Abstract: An enrichment module and method are provided for enriching a population of templated beads and separating them from non-templated beads. The method can include hybridizing a templated bead with an enrichment bead to form a complex, trapping the complex in a filtration medium, washing non-templated beads through the filtration medium while retaining the complex, and then eluting the templated bead from the complex. The module can include a column for enrichment and filtration material exhibiting desired size-exclusion properties.Type: ApplicationFiled: April 8, 2010Publication date: May 26, 2011Applicant: APPLIED BIOSYSTEMS, LLCInventors: Aldrich N. K. LAU, Achim KARGER, Patrick KINNEY, James NURSE, Aftab KEVAL, Douglas GREINER, Carmen GJERSTAD
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Publication number: 20100261229Abstract: An emulsion generation apparatus and method for forming an emulsion are provided wherein a customized impeller design is adapted to form an emulsion with a desired droplet size that defines a desired volume. The emulsion generation apparatus provides improved uniformity in emulsion preparation and may be used to create large or small volume emulsions rapidly and reproducibly. A system and method are also provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions and other reactions. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.Type: ApplicationFiled: April 8, 2010Publication date: October 14, 2010Applicant: APPLIED BIOSYSTEMS, LLCInventors: Aldrich N. K. LAU, Christine LAMBERT, Achim KARGER, Madison JORDAN
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Publication number: 20100220321Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: ApplicationFiled: August 18, 2009Publication date: September 2, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Publication number: 20100113285Abstract: A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.Type: ApplicationFiled: April 28, 2009Publication date: May 6, 2010Applicant: Life Technologies CorporationInventors: H. Pin Kao, Ian A. Harding, Kathleen C. Hayashibara, Achim Karger, Fiona C. Laird Hyland, Mark F. Oldham, Omead Ostadan, Mark R. Pratt, Junko F. Stevens, Austin B. Tomaney, Greg Young