Abstract: An additive manufacturing method, an additive manufacturing system (1200), a support material for additive manufacturing, an assembly of the support material and a structure material, and a product thereof are provided. The method comprises depositing, by a nozzle (1210a), a structure material into a support material based on a computer model of an object, thereby forming a portion of the object. Image data of at least the portion of the object can be obtained in-process by a detector (1240). The image data is compared to the computer model. Based on the comparison, the method can comprise modifying the computer model, modifying a print parameter, modifying machine path instructions for an additive manufacturing machine that comprises the nozzle, aborting the additive formation, indicating a discrepancy, indicating validation of the shape, or a combination thereof. The depositing of the structure material is repeated by the nozzle (1210a) as necessary to additively form the object.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for these assays.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Abstract: The present invention relates to the arrangement of one or more cells in a medium or on a substrate through the use of boundary conditions, which are changes in local environment compared to the medium or substrate alone or cause an alteration of cell response upon interaction of a cell with the boundary condition.

Abstract: An article has a surface topography for resisting bioadhesion of organisms and includes a base article having a surface. A composition of the surface includes a polymer. The surface has a topography comprising a pattern defined by a plurality of spaced apart features attached to or projected into the base article. The plurality of features each have at least one microscale dimension and at least one neighboring feature having a substantially different geometry. An average feature spacing between adjacent ones of the features is between 10 ?m and 100 ?m in at least a portion of the surface. The surface topography can be numerically represented using at least one sinusoidal function. In one embodiment, the surface can comprise a coating layer disposed on the base article.

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for these assays.

Abstract: The present invention provides methods of generating and devices of patterned soft substrates, on which cells may be seeded, as well as methods of using these substrates. Devices containing these patterned soft substrates are also provided.

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

Abstract: The present invention provides biological pacemakers or AV-node bypasses The biological pacemakers or AV-node bypasses of the invention are useful for the treatment of, inter alia, cardiac arrhythmias and AV-node conduction defects.

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

Abstract: The present invention provides methods for generating relevant in vitro models of engineered innervated tissue, as well as uses of such tissues.

Abstract: The present invention generally relates to a population of committed ventricular progenitor (CVP) cells and their use to generate a tissue engineered myocardium, in particular two dimensional tissue engineered myocardium which is comparable to functional ventricular heart muscle. One embodiment of present invention provides a composition and methods for the production of a tissue engineered myocardium which has functional properties of cardiac muscle, such as contractibility (e.g. contraction force) and numerous properties of mature fully functional ventricular heart muscle tissue. In particular, in one embodiment, a composition comprising the tissue engineered myocardium comprises committed ventricular progenitor (CVP) cells seeded on a free-standing biopolymer structure to form functional ventricular myocardium tissue.

Abstract: The present invention provides methods of generating and devices of patterned soft substrates, on which cells may be seeded, as well as methods of using these substrates. Devices containing these patterned soft substrates are also provided.

Abstract: The present invention relates to the arrangement of one or more cells in a medium or on a substrate through the use of boundary conditions, which are changes in local environment compared to the medium or substrate alone or cause an alteration of cell response upon interaction of a cell with the boundary condition.