Patents by Inventor Aihua Deng

Aihua Deng has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA assembly method and its application
    Patent number: 11597958
    Abstract: The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: March 7, 2023
    Assignee: NANJING ZHONGKEYOUZI INSTITUTE OF BIOTECHNOLOGY CO., LTD
    Inventors: Shuwen Liu, Tingyi Wen, Yun Zhang, Aihua Deng
  • DNA ASSEMBLY METHOD AND ITS APPLICATION
    Publication number: 20200208188
    Abstract: The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.
    Type: Application
    Filed: December 23, 2019
    Publication date: July 2, 2020
    Inventors: Shuwen LIU, Tingyi WEN, Yun ZHANG, Aihua DENG
  • Method for introducing an exogenous DNA by overcoming the restriction modification barrier of a target bacterium
    Patent number: 9605268
    Abstract: Disclosed is a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method includes the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A, 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule, and 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: March 28, 2017
    Assignee: Institute of Microbiology, Chinese Academy of Sciences
    Inventors: Tingyi Wen, Guoqiang Zhang, Aihua Deng
  • METHOD FOR INTRODUCING AN EXOGENOUS DNA BY OVERCOMING THE RESTRICTION MODIFICATION BARRIER OF A TARGET BACTERIUM
    Publication number: 20150050740
    Abstract: The present invention discloses a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method provided in the present invention comprises the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A; 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule; 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium. The experiments of the invention have demonstrated that the invention has a high transformation efficiency compared to prior methods for enabling genetic manipulation by overcoming the restriction modification barrier of the bacterium.
    Type: Application
    Filed: February 21, 2013
    Publication date: February 19, 2015
    Inventors: Tingyi Wen, Guoqiang Zhang, Aihua Deng