Abstract: A fermentation process for preparing a postbiotic with a by-product of wheat processing includes: preparation of a material; inoculation of Paenibacillus polymyxa (P. polymyxa)+Brachybacterium paraconglomeratum (B. paraconglomeratum)+Flavobacterium pectinovorum (F. pectinovorum) as fermentation strains; and isolation and extraction of the postbiotic. Since wheat starch wastewater and wheat bran include abundant nutrient components, the wheat starch wastewater and wheat bran can be used to prepare postbiotics, which is conducive to reducing a wastewater treatment cost of a related enterprise and protecting the environment, and can turn waste into treasure and increase an added value of an agricultural product.

Abstract: Disclosed are a method for preparing exosomes from bovine placenta, lyophilized powder of the exosomes, as well as a composition containing exosomes from animal placenta and a preparation method and use thereof in anti-wrinkle on skin.

Abstract: The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced.

Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.

Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.

Abstract: The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced.

Abstract: The present invention provides a battery array voltage equalization device comprising: a sampling unit which samples the battery voltage signals of the battery array according to a sampling control signal; an analog-to-digital converting unit which converts the sampled voltage signals into a digital voltage signal; a control unit which generates the sampling control signal and a driving signal based on the digital voltage signal; an equalization unit which equalizes the voltage signals of the battery array based on the driving signal; a filter unit which is connected to the equalization unit and the battery array. The present invention applies the filter unit to filter out the ripple signal generated during equalization.

Abstract: The present invention provides a circuit for controlling the operation of a backup power supply, wherein the circuit comprises a switch unit, a control unit, and a processing unit; wherein the switch unit is configured to be operably coupled to an external power supply; wherein a first input terminal is configured to be operably coupled to a backup power supply, a second input terminal is configured to be operably coupled to the switch unit, the third and fourth output terminals of the control unit are configured to be operably coupled to an input terminal of the processing unit; the fifth and sixth input terminals of the control unit are configured to be operably coupled to a respective output terminal of the processing unit; and the control unit is configured to turn on and off the backup power supply.

Abstract: The present invention provides a voltage regulating device for load starting, wherein the voltage regulating device comprises a voltage regulating circuit, and a switch circuit configured to be operably coupled between an output terminal of voltage regulating circuit and a load; wherein the switch circuit is configured to disconnect the voltage regulating circuit from the load when the voltage regulating circuit connects to a DC power.

Abstract: The present invention provides a circuit for controlling the operation of a backup power supply, wherein the circuit comprises a switch unit, a control unit, and a processing unit; wherein the switch unit is configured to be operably coupled to an external power supply; wherein a first input terminal is configured to be operably coupled to a backup power supply, a second input terminal is configured to be operably coupled to the switch unit, the third and fourth output terminals of the control unit are configured to be operably coupled to an input terminal of the processing unit; the fifth and sixth input terminals of the control unit are configured to be operably coupled to a respective output terminal of the processing unit; and the control unit is configured to turn on and off the backup power supply.

Abstract: The present invention provides a battery array voltage equalization device comprising: a sampling unit which samples the battery voltage signals of the battery array according to a sampling control signal; an analog-to-digital converting unit which converts the sampled voltage signals into a digital voltage signal; a control unit which generates the sampling control signal and a driving signal based on the digital voltage signal; an equalization unit which equalizes the voltage signals of the battery array based on the driving signal; a filter unit which is connected to the equalization unit and the battery array. The present invention applies the filter unit to filter out the ripple signal generated during equalization.