Abstract: Provided is a method for liberating sugar chains from a glycoprotein, including: a step of brining a reaction solution which contains hydroxylamines (a) and a basic reagent (b) into contact with the glycoprotein to obtain a mixed solution of sugar chains liberated from the glycoprotein and the reaction solution.

Abstract: Provided are a method for preparing a glycan from a glycoprotein, and a kit and a device for preparing a glycan from a glycoprotein. The method includes (I) a step of obtaining a glycan-containing sample by bringing a glycan releasing solution that contains a hydroxylamine compound and a basic reagent into contact with the glycoprotein and releasing the glycan from the glycoprotein; (II) a step of adsorbing a glycan having a length of a monosaccharide or more on a purifying agent for purifying the glycan by bringing the glycan-containing sample into contact with the purifying agent, which is composed of a compound having a betaine structure; and (III) a step of eluting the glycan from the purifying agent.

Abstract: Provided is a method for liberating sugar chains from a glycoprotein, including: a step of brining a reaction solution which contains hydroxylamines (a) and a basic reagent (b) into contact with the glycoprotein to obtain a mixed solution of sugar chains liberated from the glycoprotein and the reaction solution.

Abstract: There is provided a membrane electrophoresis which renders possible glycan analysis of polysaccharide and glycoprotein by separating protein, glycoprotein or polysaccharide by electrophoresis, followed by carrying out a glycan-releasing treatment of the membrane used in the electrophoresis, or a membrane electrophoresis which renders possible detection of the same by an immunostaining which uses an antibody, wherein a layer containing a hydrophilic polymer is formed on a hydrophobic polymer membrane by coating the hydrophilic polymer on the whole surface of the hydrophobic polymer membrane or by soaking the hydrophobic polymer membrane in a solution of the hydrophilic polymer, which is used as a substrate for electrophoresis.

Abstract: An object of the present invention is to provide a method for conveniently analyzing sugar chain (isomer) structure using a sample of approximately 1 picomole, which is generally subjected to analysis in proteomics without using any sugar chain preparation. The present invention relates to a method for analyzing sugar chain structure, comprising a step of obtaining the fragmentation pattern of a test sugar chain through fragmentation of the test sugar chain and a step of predicting the structure of the test sugar chain through comparison of the sugar chain predicted fragmentation pattern data generated based on fragmentation pattern templates with the fragmentation pattern of the test sugar chain.

Abstract: The present invention provides a method of enzymatic reaction on a membrane by a sugar chain releasing enzyme, for an MALDI-TOF MS analysis of a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane; a method of mass spectrometry of a sugar chain in which a sugar chain, for the MALDI-TOF MS analysis of a sugar chain excised from a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane; and a method of mass spectrometry of a glycoprotein, for the MALDI-TOF MS analysis of a glycoprotein of a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane.

Abstract: An object of the present invention is to provide a system for analyzing a sugar chain structure which can determine a complete primary structure of a sugar chain simply and rapidly with a small amount of sample.

Abstract: The present invention aims to provide processes for rapidly and easily preparing compound libraries of known structures, and compound libraries prepared by said processes. A compound library of the present invention is prepared by a process for preparing a compound library in the same vessel, comprising: (1) mixing one or more donor substrates, acceptor substrates, and transferases; (2) performing a transfer reaction to reach a degree of transfer of 1%-99% by incubating the mixture; and (3) stopping the transfer reaction.

Abstract: Oligosaccharides fragmentation pattern analysis system 10 includes: a geometry optimization part for calculating an optimized structure of an ionized oligosaccharide; a binding parameter extraction part for extracting multiple kinds of binding parameters which relate to bond strengths for respective multiple candidate bonds to be the candidate of the cleavage site within the oligosaccharide from the calculation results by the geometry optimization part; a parameter conversion part for converting respective multiple kinds of binding parameters extracted at the binding parameter extraction part, into binding scores; a weight information storage part for storing the weight information representing contribution to respective bond strengths of multiple kinds of binding scores; and a total score calculating part for determining total score of multiple kinds of binding scores in each candidate bond such that the multiple kinds of binding parameters are weighed according to the weight information.

Abstract: Disclosed are glycolipid derivatives of formula (I) ##STR1## wherein X is a group selected from ##STR2## n is 0 or an integer of 1-10 and R denotes a branched hydrocarbon chain containing from 19 to 39 carbon atoms. Those compounds are useful as antiinflammatory agents, or as agents for the treatment of rheumatoid arthritis.