Patents by Inventor Akio Yamane

Akio Yamane has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20180037941
    Abstract: A method for detecting a genetic mutation is provided. The method comprising: carrying out PCR using a template DNA, a forward primer, a reverse primer and a wild-type oligonucleotide; and detecting an amplified DNA. In the method, the wild-type oligonucleotide comprises LNA or BNA, and on a DNA strand of the template, with which the wild-type oligonucleotide is hybridized, a region with which the wild-type oligonucleotide is a hybridized and a region with which the forward primer or the reverse primer is hybridized partially overlap each other, or the regions are separated from each other by 1 to 18 bases.
    Type: Application
    Filed: October 11, 2017
    Publication date: February 8, 2018
    Applicant: RIKEN GENESIS CO., LTD.
    Inventors: Akio YAMANE, Ryoko IMAGAWA, Yuan YUAN
  • Patent number: 9523119
    Abstract: The present invention relates to a method of distinguishing genotypes using PCR-PHFA including: a nucleic acid amplification step in which a mutation site-including region of a gene is amplified by a nucleic acid amplification reaction, thereby obtaining an amplification reaction solution; and a distinction step in which the amplification reaction solution obtained from the nucleic acid amplification step is mixed with a reference double-stranded nucleic acid having a specific genotype on the mutation site as well as being labeled with a labeling substance, and the mixture is subjected to a competitive strand displacement reaction, and the level of the occurrence of strand displacement is assessed so as to distinguish the identity; and the competitive strand displacement reaction is performed under a condition to suppress a polymerase extension reaction, and a genotype distinguishing kit for use in the distinct of genotypes by this method.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: December 20, 2016
    Assignee: TOPPAN PRINTING CO., LTD.
    Inventors: Akio Yamane, Mashimo Nakayama, Shiro Kitano
  • Publication number: 20150132752
    Abstract: The method of the present invention is a method for determining an HLA-A*24 group, which includes determining the HLA-A*24 group on the basis of the results of the typing of (a) a 211st base and (b) a 395th base, in which A (adenine) in the initiation codon for an HLA gene is defined as the 1st base in the genomic DNA of a test subject.
    Type: Application
    Filed: June 26, 2014
    Publication date: May 14, 2015
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: Yoichi MAKINO, Akio Yamane, Tomohiko Azuma, Masato Nakayama
  • Publication number: 20130137095
    Abstract: A method for identifying a base sequence accompanying competitive hybridization that includes a thermal denaturation subjecting a sample double-stranded nucleic acid and a reference double-stranded nucleic acid containing the same base sequence as a target base sequence to thermal denaturation treatment in a single reaction solution, a temperature lowering carrying out competitive hybridization between the sample double-stranded nucleic acid and the reference double-stranded nucleic acid by lowering the temperature of the reaction solution after the thermal denaturation, a measurement measuring a double-stranded nucleic acid formed by a nucleic acid strand that composed the reference double-stranded nucleic acid and a nucleic acid strand that composed the sample double-stranded nucleic acid, and an identification identifying identity between the reference double-stranded nucleic acid and the sample double-stranded nucleic acid based on measurement results obtained from the measurement, the temperature lowerin
    Type: Application
    Filed: March 25, 2011
    Publication date: May 30, 2013
    Inventors: Shiro Kitano, Akio Yamane, Atsushi Maruyama
  • Publication number: 20130071844
    Abstract: Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.
    Type: Application
    Filed: March 23, 2011
    Publication date: March 21, 2013
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: Yoichi Makino, Akio Yamane, Masato Nakayama
  • Publication number: 20120077193
    Abstract: The present invention relates to a method of distinguishing genotypes using PCR-PHFA including: a nucleic acid amplification step in which a mutation site-including region of a gene is amplified by a nucleic acid amplification reaction, thereby obtaining an amplification reaction solution; and a distinction step in which the amplification reaction solution obtained from the nucleic acid amplification step is mixed with a reference double-stranded nucleic acid having a specific genotype on the mutation site as well as being labeled with a labeling substance, and the mixture is subjected to a competitive strand displacement reaction, and the level of the occurrence of strand displacement is assessed so as to distinguish the identity; and the competitive strand displacement reaction is performed under a condition to suppress a polymerase extension reaction, and a genotype distinguishing kit for use in the distinct of genotypes by this method.
    Type: Application
    Filed: September 29, 2011
    Publication date: March 29, 2012
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: Akio YAMANE, Mashimo Nakayama, Shiro Kitano
  • Patent number: 8122692
    Abstract: An empty bag supply method and apparatus first lifting up with a first suction member an empty bag of which bag bottom being in contact with and positioned by a first positioning stopper. The lifted bag is then held by chucks and transported upwards so that the empty bag is vertical with the bag bottom facing upward, and then a second suction member suctions the empty bag near the bag mouth and transports it onto a conveyor with the bag mouth facing forward, so that the bag mouth of the bag is stopped and positioned by a second positioning stopper. The empty bag is then lifted up by a third suction member, held by a chuck and transported upward so that the attitude of the bag is changed to vertical with the bag mouth facing upward, and then the bag is transferred to a gripper of a packaging apparatus.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: February 28, 2012
    Assignee: Toyo Jidoki Co., Ltd.
    Inventors: Yoshiteru Inoue, Akio Yamane, Yoshikatsu Nakahara
  • Publication number: 20110059868
    Abstract: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3?-end portion a sequence (Ac?) which hybridizes a sequence (A) in the 3?-end portion of the target nucleic acid sequence, and in the 5?-side of said sequence (Ac?) a sequence (B?) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5?-side of said sequence (A) on the target nucleic acid sequence, wherein {X?(Y?Y?)}/X is in the range of ?1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac?), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y? denotes the number of bases in an intervening sequence between said sequences (Ac?) and (B?) (Y? may be zero).
    Type: Application
    Filed: August 12, 2010
    Publication date: March 10, 2011
    Applicants: RIKEN, KABUSHIKI KAISHA DNAFORM
    Inventors: Yasumasa MITANI, Akio YAMANE, Yuko SHIBATA, Yoshihide HAYASHIZAKI
  • Publication number: 20100298530
    Abstract: To provide a photocrosslinkable compound and a photocrosslinking agent which are capable of ligation of nucleic acids within a short period of time compared to the conventional cases, and to which modifications depending on the intended use can easily be made, and a method of producing the photocrosslinking agent. Nucleic acids including a group represented by the formulae I, III, IV, or V as a nucleobase; a photocrosslinking agent including the nucleic acids, a method of producing nucleic acids by reacting nucleic acids including a group represented by the formulae VI, VII, VIII, or IX as a nucleobase with an aromatic azidated product represented by the formula X.
    Type: Application
    Filed: May 14, 2010
    Publication date: November 25, 2010
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: KENZOU FUJIMOTO, TAKEHIRO AMI, CHIFUMI NAGATA, AKIO YAMANE
  • Patent number: 7803579
    Abstract: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3?-end portion a sequence (Ac?) which hybridizes a sequence (A) in the 3?-end portion of the target nucleic acid sequence, and in the 5?-side of the sequence (Ac?) a sequence (B?) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5?-side of the sequence (A) on the target nucleic acid sequence, wherein {X?(Y?Y?)}/X is in the range of ?1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac?), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y? denotes the number of bases in an intervening sequence between the sequences (Ac?) and (B?) (Y? may be zero).
    Type: Grant
    Filed: October 29, 2003
    Date of Patent: September 28, 2010
    Assignees: Riken, Kabushiki Kaisha Dnaform
    Inventors: Yasumasa Mitani, Akio Yamane, Yuko Shibata, Yoshihide Hayashizaki
  • Publication number: 20100024362
    Abstract: An empty bag supply method and apparatus first lifting up with a first suction member an empty bag of which bag bottom being in contact with and positioned by a first positioning stopper. The lifted bag is then held by chucks and transported upwards so that the empty bag is vertical with the bag bottom facing upward, and then a second suction member suctions the empty bag near the bag mouth and transports it onto a conveyor with the bag mouth facing forward, so that the bag mouth of the bag is stopped and positioned by a second positioning stopper. The empty bag is then lifted up by a third suction member, held by a chuck and transported upward so that the attitude of the bag is changed to vertical with the bag mouth facing upward, and then the bag is transferred to a gripper of a packaging apparatus.
    Type: Application
    Filed: July 30, 2009
    Publication date: February 4, 2010
    Inventors: Yoshiteru Inoue, Akio Yamane, Yoshikatsu Nakahara
  • Patent number: 7604838
    Abstract: Active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: October 20, 2009
    Assignee: Hitachi Software Engineering Co., Ltd.
    Inventors: Hisashi Ukawa, Akio Yamane
  • Publication number: 20080272507
    Abstract: In accordance with the present invention, active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
    Type: Application
    Filed: July 3, 2008
    Publication date: November 6, 2008
    Inventors: Hisashi Ukawa, Akio Yamane
  • Publication number: 20060292635
    Abstract: In accordance with the present invention, active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
    Type: Application
    Filed: May 23, 2006
    Publication date: December 28, 2006
    Inventors: Hisashi Ukawa, Akio Yamane
  • Publication number: 20060160084
    Abstract: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3?-end portion a sequence (Ac?) which hybridizes a sequence (A) in the 3?-end portion of the target nucleic acid sequence, and in the 5?-side of said sequence (Ac?) a sequence (B?) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5?-side of said sequence (A) on the target nucleic acid sequence, wherein {X?(Y?Y?)}/X is in the range of ?1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac?), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y? denotes the number of bases in an intervening sequence between said sequences (Ac?) and (B?) (Y? may be zero).
    Type: Application
    Filed: October 29, 2003
    Publication date: July 20, 2006
    Inventors: Yasumasa Mitani, Akio Yamane, Yuko Shibata, Yoshihide Hayashizaki
  • Patent number: 6306603
    Abstract: The present invention provides CD36 mutant gene and methods and kits for diagnosing diseases caused by a lipid metabolism abnormality.
    Type: Grant
    Filed: January 3, 2000
    Date of Patent: October 23, 2001
    Assignee: Wakunaga Phaemaceutical Co., Ltd.
    Inventors: Takanori Oka, Akio Yamane, Takao Tanaka
  • Patent number: 6033851
    Abstract: An object of the present invention is to suppress nonspecific extension reaction of the primer in the primer extension method.The primer extension reaction to form a nucleic acid strand complementary to a nucleic acid template strand with the use of a primer according to the present invention is characterized in that the reaction between the primer and the template strand is carried out in the presence of a nucleic acid or a derivative thereof which is complementary to said primer and has an affinity for said primer is equivalent to or less than that of said primer for the nucleic acid template strand.
    Type: Grant
    Filed: May 30, 1997
    Date of Patent: March 7, 2000
    Assignee: Wakunaga Seiyaku Kabushiki Kaisha
    Inventor: Akio Yamane
  • Patent number: 5948618
    Abstract: A nucleic acid differentiation method utilizing complementary strand-exchange reaction in competitive hybridization can clearly differentiate a site of gene mutation or a difference between genes in a target nucleic acid even when the gene mutation or difference is located near the primer binding site of the target nucleic acid.
    Type: Grant
    Filed: November 24, 1997
    Date of Patent: September 7, 1999
    Assignee: Wakunaga Seiyaku Kabushiki Kaisha
    Inventors: Takanori Oka, Akio Yamane
  • Patent number: 5792609
    Abstract: A method of detecting falciparum, tertian, quartan and ovale malaria parasites by using primers represented by the sequences (SEQ ID NO:1), (SEQ ID NO:3) to (SEQ ID NO:10):5'AAGTCATCTTTCGAGGTGAC3' (SEQ ID NO:4)5'GAATTTTCTCTTCGGAGTTTA3' (SEQ ID NO:5)5'GAGACATTCTTATATATG3' (SEQ ID NO:3)5'GAAAATTCCTTTCGGGGA3' (SEQ ID NO:1)5'CGACTAGGTGTTGGATGA3' (SEQ ID NO:6)5'GAACGAAAGTTAAGGGAGT3' (SEQ ID NO:7)5'ACTGAAGGAAGCAATCTAA3' (SEQ ID NO:8)5'TCAGATACCGTCGTAATCTT3' (SEQ ID NO:9)5'CCAAAGACTTTGATTTCTCAT3' (SEQ ID NO:10)This invention allows all of the plasmodia, which infect the human, to be detected easily, rapidly and with a high sensitivity or distinguished accurately from one another, thus permitting treatment for malaria and large-scale mass examination in the area where malaria is prevalent.
    Type: Grant
    Filed: September 11, 1995
    Date of Patent: August 11, 1998
    Assignee: Wakunaga Pharmaceutical Co., Ltd.
    Inventors: Yusuke Wataya, Akio Yamane
  • Patent number: 5741638
    Abstract: A microtiter well in which a single stranded nucleic acid including a plurality of sequences specifically hybridizalbe with a target nucleic acid is immobilized is disclosed. The single stranded nucleic acid is derived from a phage or a phage-plasmid. The microtiter well enables a target nucleic acid to be specifically detected with a high sensitivity and high efficiency and further enables the detection procedure to be automated.
    Type: Grant
    Filed: December 19, 1994
    Date of Patent: April 21, 1998
    Assignee: Wakunaga Seiyaku Kabushiki Kaisha
    Inventor: Akio Yamane