Abstract: Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use a Renilla GFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intracellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.

Abstract: Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use a Renilla GFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intracellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.

Abstract: Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use a Renilla GFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intra-cellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.

Abstract: The present invention relates to a novel biosensor. A resonance energy transfer (RET) biosensor comprising a beta(?)-arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore is described.

Abstract: A vehicle seat backrest suitable for allowing the attachment of a child seat, the backrest comprising a rigid structure and an anchoring system comprising an anchor rod to permit to a coupling member belonging to the child seat to be anchored on the anchor rod via the rear face of the rigid structure. The anchoring system comprises a rigid plate comprising retainer suitable for retaining the anchor rod by abutment contact when the coupling member of the child seat exerts forces on the said anchor rod.

Abstract: A seat for an automobile vehicle comprises a seat pan (2) and a seat back (3), the seat pan (2) featuring a seating surface that is approximately horizontal extending from the seat back (3) to a forward limit (43), the seating surface comprising a fixed seating surface (4) and a mobile front section (5) spanning at least part of the width of the forward limit (43). The front section (5) can move between a raised position in which it is aligned with the fixed seating surface (4), and a tilted position in which the front section (5) is tilted down below the fixed seating surface (4) forming ridge (44) with it that is more or less parallel with the forward limit (43), this position being suitable to accommodate a seated child.

Abstract: A cheek plate for a rigid structure of a vehicle seat, the cheek plate being constituted by a rigid plate having a central portion defined on either side by first and second side portions. The central portion has at least a first channel section, and the first side portion extends to a side end which is folded over and fastened against said first channel section so as to form at least a first box section.

Abstract: A vehicle seat backrest suitable for allowing the attachment of a child seat, the backrest comprising a rigid structure and an anchoring system comprising an anchor rod to permit to a coupling member belonging to the child seat to be anchored on the anchor rod via the rear face of the rigid structure. The anchoring system comprises a rigid plate comprising retention means suitable for retaining the anchor rod by abutment contact when the coupling member of the child seat exerts forces on the said anchor rod.

Abstract: A braking system for braking a first element (2) pivotally mounted on a pivot pin (6) that is secured to a stationary second element (3). The system includes a helical spring (18) which has a diameter adapted so that, in the assembled state, said helical spring (18) is in contact against the inside wall (15) of a sleeve (14) secured to the second element, the helical spring (18) being adapted so as to make it possible, while the first element is pivoting, to increase the diameter of the helical spring (18) so as to increase the friction force between the helical spring (18) and the sleeve (14), thereby causing the pivoting of the first element (2) to be braked.

Abstract: A seat for an automobile vehicle comprises a seat pan (2) and a seat back (3), the seat pan (2) featuring a seating surface that is approximately horizontal extending from the seat back (3) to a forward limit (43), the seating surface comprising a fixed seating surface (4) and a mobile front section (5) spanning at least part of the width of the forward limit (43). The front section (5) can move between a raised position in which it is aligned with the fixed seating surface (4), and a tilted position in which the front section (5) is tilted down below the fixed seating surface (4) forming ridge (44) with it that is more or less parallel with the forward limit (43), this position being suitable to accommodate a seated child.

Abstract: A seat pan of an automobile vehicle seat includes an anti-sub-marining cross member to limit the movement of a pelvis of a user towards the front of the seat, the cross member extending crosswise to a seat pan framework and being able to move towards the front while exerting pelvis retention forces during the movement of the pelvis and cross member towards the front of the seat. The seat pan includes energy dissipation means to control, from a predetermined threshold, forces transmitted by the cross member to the occupant of the seat. The ends of the cross member are connected to links that pivot on the seat pan framework about an axis passing through lower ends of said links so that the movement of the cross member towards the front is in the form of an arc of a circle, defined by the pivoting of the links towards the front, opposing the retention force exerted by the energy dissipation means which are connected to upper ends of the links and near the cross member.

Abstract: The invention concerns a motor vehicle seat (1) characterised in that it comprises in its seating portion (3) a part (6) controlling the deceleration of the pelvis of the passenger seated thereon, particularly during a frontal impact, consisting of an element in aluminium foam. The invention is applicable to motor vehicle seats.

Abstract: A motor vehicle seat support includes a carrier frame on which front forks and rear forks are designed to work respectively with fastening rods that are integral with the floor of the vehicle. Each one of the front and rear forks carries a mobile catch to lock each one of the forks with the corresponding rod, and a device for operating the catches to bring the latter into an unlocked position, so as to make possible the disengagement of the fork in relation to the fastening rods. The front and rear forks are carried by two lower guide rails in contact with upper guide rails braced by a base for holding a seat.

Abstract: An electronic control circuit of the type including a microprocessor, a circuit for monitoring the operational state of the microprocessor connected directly to a first output of the microprocessor, and a warning light of the control circuit and its input and output peripherals. The warning light is connected to a second output of said microprocessor through a starter and protection circuit and thus diagnostic data are available in series at the input terminal of said warning light which is also the output of said starter and protection circuit.