Patents by Inventor Alan L. Rockwood
Alan L. Rockwood has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11969670Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.Type: GrantFiled: July 26, 2021Date of Patent: April 30, 2024Assignee: ARUP Laboratories, Inc.Inventors: Mark M. Kushnir, Alan L. Rockwood
-
Publication number: 20220040597Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.Type: ApplicationFiled: July 26, 2021Publication date: February 10, 2022Inventors: Mark M. Kushnir, Alan L. Rockwood
-
Patent number: 11071927Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.Type: GrantFiled: November 15, 2018Date of Patent: July 27, 2021Assignee: ARUP Laboratories, Inc.Inventors: Mark M. Kushnir, Alan L. Rockwood
-
Patent number: 10627401Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.Type: GrantFiled: September 21, 2015Date of Patent: April 21, 2020Assignee: University of Utah Research FoundationInventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Publication number: 20190361040Abstract: Methods for measuring and analyzing parathyroid hormone-related peptide (PTHrP) using LC-MS/MS, including applications of the methods thereof, are disclosed and discussed. Such methods can include, along with the use of an isotope-labeled internal standard, purifying PTHrP from a biological sample, proteolytically digesting the PTHrP, and measuring specific digestion products using the using LC-MS/MS.Type: ApplicationFiled: March 25, 2019Publication date: November 28, 2019Inventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Publication number: 20190329153Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.Type: ApplicationFiled: November 15, 2018Publication date: October 31, 2019Inventors: Mark M. Kushnir, Alan L. Rockwood
-
Patent number: 10241119Abstract: Methods for measuring and analyzing parathyroid hormone-related peptide (PTHrP) using LC-MS/MS, including applications of the methods thereof, are disclosed and discussed. Such methods can include, along with the use of an isotope-labeled internal standard, purifying PTHrP from a biological sample, proteolytically digesting the PTHrP, and measuring specific digestion products using the using LC-MS/MS.Type: GrantFiled: May 9, 2016Date of Patent: March 26, 2019Assignee: University of Utah Research FoundationInventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Publication number: 20180342381Abstract: Processes and methods for modeling non-linear calibration behavior resulting from isotopic interference between a target analyte and an internal standard during a mass spectrometry operation are disclosed and described. In some embodiments, a correction to instrument data obtained during the mass spectrometry operation can be made. Such a correction may entail determining, in some cases experimentally determining, one or two constants, and a single adjustable parameter for each analyte/internal standard pair.Type: ApplicationFiled: March 12, 2014Publication date: November 29, 2018Inventors: Geoffrey S. Rule, Zlatuse D. Clark, Bingfang Yue, Alan L. Rockwood
-
Patent number: 9834578Abstract: The present disclosure provides methods and composition involving increased sensitivity of compounds using mass spectrometry. In one embodiment, a method of increasing the sensitivity for detection of a carbonyl group-containing compound by mass spectrometry can comprise derivatizing the carbonyl group-containing compound with an O-substituted hydroxylamine thereby producing an oxime, resulting in enhanced sensitivity of detection by mass spectrometry, as compared to the underivatized carbonyl group-containing compound. Additionally, a method for assaying a carbonyl group-containing compound can comprise reacting the carbonyl group-containing compound in a sample with an O-substituted hydroxylamine to produce an oxime and performing analysis with mass spectrometric detection of the oxime by a mass spectrometry instrument.Type: GrantFiled: May 24, 2011Date of Patent: December 5, 2017Assignee: University of Utah Research FoundationInventors: Bingfang Yue, Alan L. Rockwood, Mark M. Kushnir
-
Publication number: 20160327577Abstract: Methods for measuring and analyzing parathyroid hormone-related peptide (PTHrP) using LC-MS/MS, including applications of the methods thereof, are disclosed and discussed. Such methods can include, along with the use of an isotope-labeled internal standard, purifying PTHrP from a biological sample, proteolytically digesting the PTHrP, and measuring specific digestion products using the using LC-MS/MS.Type: ApplicationFiled: May 9, 2016Publication date: November 10, 2016Applicant: University of Utah Research FoundationInventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Publication number: 20160077092Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.Type: ApplicationFiled: September 21, 2015Publication date: March 17, 2016Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATIONInventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Patent number: 9140695Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.Type: GrantFiled: November 14, 2012Date of Patent: September 22, 2015Assignee: University of Utah Research FoundationInventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
-
Publication number: 20120126107Abstract: The present disclosure provides methods and composition involving increased sensitivity of compounds using mass spectrometry. In one embodiment, a method of increasing the sensitivity for detection of a carbonyl group-containing compound by mass spectrometry can comprise derivatizing the carbonyl group-containing compound with an O-substituted hydroxylamine thereby producing an oxime, resulting in enhanced sensitivity of detection by mass spectrometry, as compared to the underivatized carbonyl group-containing compound. Additionally, a method for assaying a carbonyl group-containing compound can comprise reacting the carbonyl group-containing compound in a sample with an O-substituted hydroxylamine to produce an oxime and performing analysis with mass spectrometric detection of the oxime by a mass spectrometry instrument.Type: ApplicationFiled: May 24, 2011Publication date: May 24, 2012Inventors: Bingfang Yue, Alan L. Rockwood, Mark M. Kushnir
-
Patent number: 7375320Abstract: A virtual ion trap that uses electric focusing fields instead of machined metal electrodes that normally surround the trapping volume, wherein two opposing surfaces include a plurality of uniquely designed and coated electrodes, and wherein the electrodes can be disposed on the two opposing surfaces using plating techniques that enable much higher tolerances to be met than existing machining techniques.Type: GrantFiled: May 24, 2007Date of Patent: May 20, 2008Assignee: Brigham Young UniversityInventors: Edgar D. Lee, Alan L. Rockwood, Randall Waite, Stephen A. Lammert, Milton L. Lee
-
Patent number: 7227138Abstract: A virtual ion trap that uses electric focusing fields instead of machined metal electrodes that normally surround the trapping volume, wherein two opposing surfaces include a plurality of uniquely designed and coated electrodes, and wherein the electrodes can be disposed on the two opposing surfaces using plating techniques that enable much higher tolerances to be met than existing machining techniques.Type: GrantFiled: June 28, 2004Date of Patent: June 5, 2007Assignee: Brigham Young UniversityInventors: Edgar D. Lee, Alan L. Rockwood, Randall Waite, Stephen A. Lammert, Milton L. Lee
-
Patent number: 7199362Abstract: A cross-flow ion mobility analyzer (CIMA) that includes a component of gas flow that opposes an electric field that is established within a channel, wherein ions are carried through the channel, wherein ions of a specific mobility are trapped by the opposing electric field and flow field within the channel and are detected when the ions reach the end of the channel, wherein a detector at the end of the channel sees a continuous stream of mobility-selected ions, and wherein different ions are selected by modifying the electric field and/or the velocity of the flow field.Type: GrantFiled: April 9, 2004Date of Patent: April 3, 2007Assignee: Brigham Young UniversityInventors: Alan L. Rockwood, Edgar D. Lee, Nosa Agbonkonkon, Milton L. Lee
-
Patent number: 7158903Abstract: The present invention provides methods for high throughput analysis of analytes in complex mixtures for unresolved chromatographic peaks including specific embodiment for summing intensities for each mass transition of interest over a selected chromatographic peak (50) to generate a signal corresponding to total intensity for each transition (55, 60). The intensities are deconvoluted into intensities of individual analytes (65, 70), based on branching ratios acquired from authentic standards, and a comparison to calibration curve is performed to obtain a quantitative concentration measurement of a particular analyte in a sample.Type: GrantFiled: March 14, 2003Date of Patent: January 2, 2007Assignee: University of UtahInventors: Mark M. Kushnir, Alan L. Rockwood, Gordon J. Nelson
-
Patent number: 7075070Abstract: A single set of electrodes, wherein different electrical potentials are applied to the single set of electrodes at different times in order to perform both ion mobility-based spectrometry and mass spectrometry (MS) on a sample of ions, wherein the ions are processed by performing ion mobility-based spectrometry and mass spectrometry in any sequence, any number of times, and as isolated or superposed procedures in order to trap, separate, fragment, and/or analyze charged particles and charged particles derived from atoms, molecules, particles, sub-atomic particles and ions.Type: GrantFiled: June 21, 2004Date of Patent: July 11, 2006Assignee: Brigham Young UniversityInventors: Edgar D. Lee, Alan L. Rockwood, Milton L. Lee, Samuel E. Tolley
-
Patent number: 7060987Abstract: A radio-frequency quadrupole ion guide having a symmetrical magnetic field disposed along an axis of the ion guide, wherein the system provides prolonged interaction between electrons and uncharged compounds within an ionization volume of the ion guide, resulting in enhanced ion creation.Type: GrantFiled: March 3, 2004Date of Patent: June 13, 2006Assignee: Brigham Young UniversityInventors: Edgar D. Lee, Alan L. Rockwood, Bingfang Yue, Milton L. Lee
-
Patent number: 6992299Abstract: A method and apparatus for focusing ions for delivery to an ion detection device using an aerodynamic ion focusing system that uses a high-velocity converging gas flow at an entrance aperture to focus an ion plume by reducing spreading and increasing desolvation of ions, and wherein a voltage is applied to at least a portion of the aerodynamic ion focusing system to assist in the focusing and delivery of ions to the ion detection device.Type: GrantFiled: December 18, 2003Date of Patent: January 31, 2006Assignee: Brigham Young UniversityInventors: Edgar D. Lee, Milton L. Lee, Alan L. Rockwood, Li Zhou