Abstract: Provided herein are compositions comprising a CRISPR array comprising one or more crRNA sequences and a 5? direct repeat (DR) sequence linked to a barcode guide RNA (bcgRNA), wherein the bcgRNA comprises from 5? to 3? (a) a barcode sequence, and (b) a reverse-transcription handle. Also provided are methods that comprise using the described CRISPR arrays to introduce one or perturbations in a single cell transcriptome.

Abstract: An electrolyte composition containing (i) at least one aprotic organic solvent; (ii) at least one conducting salt; (iii) at least one silyl ester phosphonate containing the structure of formula (I), wherein T is selected from (Ia) and (Ib); and (iv) optionally one or more additives.

Abstract: Provided herein are compositions and methods comprising modified crRNA comprising a spacer sequence and a direct repeat sequence, wherein the crRNA comprises one or more chemically modified nucleotides. Also provided are methods of enhancing modulation of gene transcripts in a cell, the method comprising introducing into the cell a modified crRNA as described herein, and a Cas13 polypeptide, an mRNA encoding a Cas13 polypeptide, and/or a recombinant expression vector comprising a nucleotide sequence encoding a Cas13 polypeptide, wherein the modified crRNA guides the Cas13 polypeptide to the target RNA sequence, and wherein the modified crRNA induces regulation of the target RNA with an enhanced activity relative to a corresponding unmodified crRNA.

Abstract: A Class 2, Type VI clustered regularly interspaced short palindromic repeat (CRISPR) RNA (crRNA) which comprises a direct repeat (DR) stem loop sequence and a guide or spacer sequence, is provided characterized by a DR selected from those of Table 9. Also described is are methods for generating, selecting, characterizing and optimizing a clustered regularly interspaced short palindromic repeats (CRISPR) RNA (crRNA) for use in the CRISPR-Cas13d system described herein. Also provided is a screening method to identify crRNA particularly suited for use with specified targets. Further, the invention includes non-naturally occurring, synthesized or engineered crRNAs as described herein along with nucleic acid molecule, vectors, RNPs, cells, libraries, and compositions comprising the same, and uses thereof in treating a disease or in functionally screening a gene.

Abstract: Process for making a cathode comprising the following steps (a) Providing a cathode active material selected from layered lithium transition metal oxides, lithiated spinels, lithium transition metal phosphate with olivine structure, and lithium nickel-cobalt aluminum oxides, (b) treating said cathode active material with an oligomer bearing units according to general formula (I a), wherein R1 are the same or different and selected from hydrogen and C1-C4-alkyl, aryl, and C4-C7-cycloalkyl, R2 and R3 are selected independently at each occurrence from phenyl and C1-C8-alkyl, C4-C7-cycloalkyl, C1-C8-haloalkyl, OPR1(O)—*, and —(CR92)p—Si(R2)2—* wherein one or more non-vicinal CR92-groups may be replaced by oxygen, R9 is selected independently at each occurrence from H and C1-C4-alkyl, and p is a variable from zero to 6, and wherein the overall majority of R2 and R3 is selected from C1-C8-alkyl, and, optionally, at least one of carbon in electrically conductive form and, optionally, a binder, (c) appl

Abstract: A non-aqueous electrolyte composition containing (i) at least one aprotic organic solvent; (ii) a compound of formula (I) (iii) at least one ion containing conducting salt; and (iv) optionally one or more additives.

Abstract: In some examples, a computing device can define a marked area of an image of analog imagery captured by the computing device, separate the analog imagery of the image from a background of the image, and generate a small vector image that includes the analog imagery of the image.

Abstract: An electrochemical cell comprising (A) an anode comprising at least one anode active material, (B) a cathode comprising at least one cathode active material selected from mixed lithium transition metal oxides containing Mn and at least one second transition metal; lithium intercalating mixed oxides containing Ni, Al and at least one second transition metal; LiNiPO4; LiMnPO4; and LiCoPO4; (C) an electrolyte composition containing (i) at least one aprotic organic solvent; (ii) at least one lithium ion containing conducting salt; (iii) a compound of formula (I).

Abstract: An electrolyte composition containing (i) at least one aprotic organic solvent; (ii) at least one conducting salt; (iii) at least one silyl ester phosphonate containing the structure of formula (I), wherein T is selected from (Ia) and (Ib); and (iv) optionally one or more additives.

Abstract: An electrochemical cell has a cathode active material selected from mixed lithium transition metal oxides containing Mn and at least one second transition metal; lithium intercalating mixed oxides containing Ni, Al and at least one second transition metal; and lithium metal phosphates, wherein the outer surface of the particulate cathode active material is at least partially coated with an oxide selected from transition metal oxides, lanthanide oxides, and oxides of metals and half metals of groups 2, 13, and 14 of the periodic system; and an electrolyte composition containing at least one silyl ester phosphonate of formula (I) and at least one silyl ester phosphonate of formula (II)

Abstract: Phosphonate based lithium complexes of formula (I) and their use in electrolyte compositions for electrochemical cells.

Abstract: Process for making a cathode comprising the following steps (a) Providing a cathode active material selected from layered lithium transition metal oxides, lithiated spinels, lithium transition metal phosphate with olivine structure, and lithium nickel-cobalt aluminum oxides, (b) treating said cathode active material with an oligomer bearing units according to general formula (I a), wherein R1 are the same or different and selected from hydrogen and C1-C4-alkyl, aryl, and C4-C7-cycloalkyl, R2 and R3 are selected independently at each occurrence from phenyl and C1-C8-alkyl, C4-C7-cycloalkyl, C1-C8-haloalkyl, OPR1(O)—*, and —(CR92)p—Si(R2)2—* wherein one or more non-vicinal CR92 groups may be replaced by oxygen, R9 is selected independently at each occurrence from H and C1-C4-alkyl, and p is a variable from zero to 6, and wherein the overall majority of R2 and R3 is selected from C1-C8-alkyl, and, optionally, at least one of carbon in electrically conductive form and, optionally, a binder, c) applying a slurry o

Abstract: In some examples, a computing device can define a marked area of an image of analog imagery captured by the computing device, separate the analog imagery of the image from a background of the image, and generate a small vector image that includes the analog imagery of the image.

Abstract: An electrochemical cell comprising (A) an anode comprising at least one anode active material, (B) a cathode comprising at least one cathode active material selected from mixed lithium transition metal oxides containing Mn and at least one second transition metal; lithium intercalating mixed oxides containing Ni, Al and at least one second transition metal; LiNiPO4; LiMnPO4; and LiCoPO4; (C) an electrolyte composition containing (i) at least one aprotic organic solvent; (ii) at least one lithium ion containing conducting salt; (iii) a compound of formula (I).

Abstract: Phosphonate based lithium complexes of formula (I) and their use in electrolyte compositions for electrochemical cells.

Abstract: A non-aqueous electrolyte composition containing (i) at least one aprotic organic solvent; (ii) a compound of formula (I) (iii) at least one ion containing conducting salt; and (iv) optionally one or more additives.

Abstract: An electrochemical cell comprising (C) an anode containing at least one anode active material, (D) a cathode comprising at least one cathode active material containing Ni, and (C) an electrolyte composition containing (i) at least one aprotic organic solvent; (ii) at least one lithium ion containing conducting salt; (iii) CF2H(CF2)3CH2OCF2CF2H; and (iv) optionally one or more additives.

Abstract: The present invention relates to a group of polycationic amphiphilic cyclooligosaccharides which, due to their molecular structure, may be used as molecular transporters to the interior of cells. Furthermore, the present invention also relates to the use of said compounds in the preparation of a medicament, to the use of this medicament for gene therapy and to a pharmaceutical composition that comprises one of said compounds.

Abstract: A solution for preserving fresh flowers, fruits or vegetables without the use of refrigeration is produced by synthesizing certain decay-retarding molecular agents, such as certain enzymes, proteins, carbohydrates, and/or fats, of one of a flowering plant, a flower, a fruit or a vegetable and combining the synthetic molecular agents with an application agent to facilitate adhesion of the synthetic molecular agents to a fresh flower, fruit or vegetable. The molecular agents may be synthesized (i.e., artificially produced) using genetic engineering, chemical composition, man-made substances, and/or natural substances. The molecular agents are preferably isolated after deformulating a solution containing juice extracted from a fruit, flower or vegetable, a solvent, and remains of the fruit, flower or vegetable from which the juice was extracted. The application agent preferably comprises a powder mixed with another solvent, such as water.

Abstract: A method of producing and using a solution, and the solution itself, for extending the shelf life of fresh flowers, fruits and vegetables after they are harvested which includes the steps of extracting juice from the flowers, flowering plant parts, fruits or vegetables, filtering the juice, disintegrating the parts from which the juice is extracted, extracting an enzyme or enzymes from the parts using a solvent without the denaturing the enzymes, removing the parts and mixing the remaining ingredients with the extracted juice.