Patents by Inventor Ales Strancar
Ales Strancar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230302381Abstract: A method for removal of host cell DNA from a sample containing a species of desired protein, virus, or extracellular vesicle comprising the steps of: Loading a substrate bearing an anionic metal affinity ligand with a metal ion, Equilibrating the substrate with a buffer having a pH in the range of pH 6 to pH 10, and a salt concentration in a concentration range up to 1 M which salt is not forming a chemical complex with the anionic metal affinity ligand, Contacting the sample with the metal-loaded anionic metal affinity substrate, Separating the substrate from the sample, wherein the sample has reduced content of contaminating DNA.Type: ApplicationFiled: August 18, 2021Publication date: September 28, 2023Inventors: Peter S. GAGNON, Sara Drmota PREBIL, Maja LESKOVEC, Ales STRANCAR
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Publication number: 20230294017Abstract: A method for separating full Adeno-associated virus (AAV) capsids from empty AAV capsids in a buffered mixture comprising full AAV capsids, empty AAV capsids, comprising the steps of contacting the buffered mixture with a first substrate bearing a metal affinity ligand attached to the first substrate, said metal affinity ligand having the ability to complex metal ions via three or more nitrogen atoms, separating empty AAV capsids from full AAV capsids by eluting with a pH gradient, a salt gradient, a metal ion gradient or a combination thereof in the presence of multivalent cations bound to the metal affinity ligand to obtain a purified full AAV capsid fraction.Type: ApplicationFiled: August 18, 2021Publication date: September 21, 2023Inventors: Maja LESKOVEC, Sara Drmota PREBIL, Rok ZIGON, Ales STRANCAR, Peter S. GAGNON
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Publication number: 20230227791Abstract: A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.Type: ApplicationFiled: June 4, 2021Publication date: July 20, 2023Inventors: Peter S. GAGNON, Maja LESKOVIC, Sara Drmota PREBIL, Ales STRANCAR
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Patent number: 11339377Abstract: The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.Type: GrantFiled: November 8, 2018Date of Patent: May 24, 2022Assignee: BLUESKY IMMUNOTHERAPIES GMBHInventors: Markus Wolschek, Manfred Reiter, Ales Strancar, Mojca Tajnik Sbaizero
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Publication number: 20210388058Abstract: A method for manufacturing a fraction comprising the proteins lactoferrin and/or lactoperoxidase from a source containing at least one of these proteins wherein the source is selected from the group consisting of milk, colostrum, acid or sweet whey, by means of a chromatographic separation process with a monolithic column having cation exchanger properties, wherein in the separation process a pH gradient or a combined pH and salt gradient elution is employed after loading the source to the column. Further disclosed is a composition of matter comprising lactoferrin having a C value of >60% and A value of >1% or lactoperoxidase.Type: ApplicationFiled: November 6, 2019Publication date: December 16, 2021Inventors: Marko KETE, Blaz LOKAR, Maja Zupancic JUSTIN, Ales STRANCAR
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Publication number: 20200332264Abstract: The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.Type: ApplicationFiled: November 8, 2018Publication date: October 22, 2020Inventors: Markus WOLSCHEK, Manfred REITER, Ales STRANCAR, Mojca Tajnik SBAIZERO
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Patent number: 10570376Abstract: A process for the purification of influenza virus or derivative thereof requires providing a source of influenza virus or derivative thereof, optionally subjecting the source to a pre-purification step, followed by subjecting the source to at least one chromatographic step on chromatographic material selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths, and collecting fractions eluted from the chromatographic material that contain the influenza virus or derivative thereof, excluding sulfuric ester of cellulose or cross-linked polysaccharides.Type: GrantFiled: July 6, 2015Date of Patent: February 25, 2020Assignees: BIA SEPARATIONS D.O.O., BAXTER HEALTHCARE SAInventors: Matjaz Peterka, Ales Strancar, Marko Banjac, Petra Kramberger, Elisabeth Roethl, Thomas Muster
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Publication number: 20160002606Abstract: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus or derivative thereof; optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatographic materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths; collecting eluting influenza virus or derivatives thereof containing fractions with the proviso that sulfuric ester of cellulose or cross-linked polysaccharides are excluded.Type: ApplicationFiled: July 6, 2015Publication date: January 7, 2016Inventors: Matjaz PETERKA, Ales STRANCAR, Marko BANJAC, Petra KRAMBERGER, Elisabeth ROETHL, Thomas MUSTER
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Publication number: 20100158944Abstract: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus or derivative thereof; optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatographic materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths; collecting eluting influenza virus or derivatives thereof containing fractions with the proviso that sulfuric ester of cellulose or cross-linked polysaccharides are excluded.Type: ApplicationFiled: July 6, 2007Publication date: June 24, 2010Inventors: Matjaz Peterka, Ales Strancar, Marko Banjac, Petra Kramberger, Elisabeth Rothl, Thomas Muster
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Patent number: 6893856Abstract: Disclosed is a process for preparing agents containing virus-inactivated vitamin K-dependent plasma components as well as protein C, protein S, factors II, VII, IX and/or X as well as combinations thereof, such as, for example, PPSB preparations, wherein a source containing these components is subjected to a appropriate separation procedures, especially by using membrane-chromatographic methods.Type: GrantFiled: May 2, 2002Date of Patent: May 17, 2005Assignee: Octapharma AGInventors: Djuro Josic, Ales Strancar
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Patent number: 6736973Abstract: A porous self-supporting structure comprising at least two porous components A and B and the porous component B embraces the porous component A, wherein (i) surfaces of pores of the at least two porous components A and B are provided with chemical moieties for interaction with substances passing the pores and (ii) the pores of the porous components comprise a uniform multimodal pore size distribution through the entire polymeric structure.Type: GrantFiled: July 26, 2000Date of Patent: May 18, 2004Assignee: BIA Separations d.o.o.Inventors: Ales Podgornik, Milos Barut, Ales Strancar, Djuro Josic
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Publication number: 20040002081Abstract: The invention relates to a process for isolating and purifying a polynucleotide on a manufacturing scale which uses a chromatographic separation process comprising a combination of two different chromatographic steps selected from hydrophobic interaction chromatography, polar interaction chromatography and anion exchange chromatography. In at least one of the two steps the chromatographic support is a porous monolithic bed. The invention also relates to a chromatographic device and its use for isolating and purifying a polynucleotide of interest, in particular plasmid DNA, on manufacturing scale.Type: ApplicationFiled: December 18, 2002Publication date: January 1, 2004Applicant: Boehringer Ingelheim International GmbH and BIA Separations d.o.o.Inventors: Jochen Urthaler, Roman Necina, Janez Jancar, Ales Podgornik, Milos Barut, Ales Strancar, Mojca Merhar
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Patent number: 6664305Abstract: A process of manufacturing of a chromatography material comprising the steps of (i) reacting a polymerizable at least bifunctional monomer A with a ligand also having a functional group which binds covalently with one of the functional groups of said polymerizable bifunctional monomer A, (ii) to form a compound B comprising at least one polymerizable functional moiety (iii) polymerizing the compound B essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting or (iv) reacting the ligand and a spacer via a covalent bond which is cleavable to form a ligand-spacer conjugate and binding the ligand-spacer conjugate to the surface of a chromatographic support or reacting the ligand-spacer conjugate via a covalent bond to the at least bi-functional monomer A and polymerizing it essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting.Type: GrantFiled: October 28, 2002Date of Patent: December 16, 2003Assignee: BIA Separations d.o.o.Inventors: Alois Jungbauer, Rainer Hahn, Ales Podgornik, Milos Barut, Ales Strancar
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Publication number: 20030125204Abstract: A process of manufacturing of a chromatography material comprising the steps ofType: ApplicationFiled: October 28, 2002Publication date: July 3, 2003Inventors: Alois Jungbauer, Rainer Hahn, Ales Podgornik, Milos Barut, Ales Strancar
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Publication number: 20020182582Abstract: Disclosed is a process for preparing agents containing virus-inactivated vitamin K-dependent plasma components as well as protein C, protein S, factors II, VII, IX and/or X as well as combinations thereof, such as, for example, PPSB preparations, wherein a source containing these components is subjected to a appropriate separation procedures, especially by using membrane-chromatographic methods.Type: ApplicationFiled: May 2, 2002Publication date: December 5, 2002Applicant: Octapharma AGInventors: Djuro Josic, Ales Strancar
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Publication number: 20020045240Abstract: Disclosed is a process for preparing agents containing virus-inactivated vitamin K-dependent plasma components as well as protein C, protein S, factors II, VII, IX and/or X as well as combinations thereof, such as, for example, PPSB preparations, wherein a source containing these components is subjected to appropriate separation procedures, especially by using membrane-chromatographic methods.Type: ApplicationFiled: August 29, 1996Publication date: April 18, 2002Inventors: DJURO JOSIC, ALES STRANCAR
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Patent number: 6319401Abstract: A device for the rapid separation and/or conversion of substrates is disclosed herein. The device include a porous tube (2) disposed inside a tubular sample distributor (3). The tubular sample distributor (3) includes channel structures on its inner surface and is disposed inside a tubular body (4). A tubular sample collector (1) having channel structures on its outer surface is disposed inside the porous tube (2). A circular sample distributor (7) is provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample distributor (3). A circular sample collector (8) is also provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample collector (1). The hollow body (4) is arranged between securing means (5) that provide a sample inlet and outlet in communication with the circular sample distributor (7) and circular sample collector (8), respectively.Type: GrantFiled: March 26, 1999Date of Patent: November 20, 2001Assignee: BIA Separations d.o.o.Inventors: Djuro Josic, Primoz Koselj, Ales Podgronik, Ales Strancar
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Patent number: 5972218Abstract: An apparatus for the conversion of substrates both for analytical and preparative purposes in a flow-through and cross-flow reactor having compact porous discs and/or compact porous tubes as carrier for conversion reaction or preparative separations which compact porous discs and/or compact porous tubes are modified with substances being capable of specifically binding and/or converting said substrates by distributing the samples to be converted and/or preparatively separated and collecting the converted and/or separated substrates preferably in a low volume, wherein the distribution of the sample does not affect the carrier adversely.Type: GrantFiled: August 23, 1995Date of Patent: October 26, 1999Assignee: BIA d.o.o. Laboratory and Process Equipment Co., Ltd.Inventors: Djuro Josic, Primoz Koselj, Ales Podgronik, Ales Strancar