Abstract: The present invention provides a method for the isolation of sperm DNA from swabs taken from rape victims without having to perform a change in buffers. Non-sperm cells from the victim are digested with an enzyme and solubilized, and then in the same buffer an enzyme capable of digesting soluble DNA is added and the victim's DNA is degraded, leaving only the rapist's DNA intact. Since no change of buffer is needed, no centrifugation or filtration steps are needed. The inventive method has utility particularly in the forensic science field.

Abstract: The present invention relates to a method for quantifying residual host cell genomic DNA in recombinant protein biologics using quantitative real time PCR.

Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as mass difference.

Abstract: The present invention discloses a new method for detecting or quantifying a truncating mutation of a target gene in a subject, said method relying on the in vitro compartmentalization of single genetic constructs in aqueous droplets of a water-in-oil emulsion.

Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.

Abstract: The present invention provides a method for the isolation of sperm DNA from swabs taken from rape victims without having to perform a change in buffers. Non-sperm cells from the victim are digested with an enzyme and solubilized, and then in the same buffer an enzyme capable of digesting soluble DNA is added and the victim's DNA is degraded, leaving only the rapist's DNA intact. Since no change of buffer is needed, no centrifugation or filtration steps are needed. The inventive method has utility particularly in the forensic science field.

Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.

Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.

Abstract: In various aspects, the present invention provides novel and effective methods and kits for the isolation of sperm and sperm DNA from samples having at least one other cell type, or having the DNA of one other cell type. More specifically, a process for isolating sperm DNA from a mixture of sperm and non-sperm cells by filtration is provided. DNA from non-sperm cells is solubilized by selective lysis, and the intact sperm are retained on a filter, washed, and then treated in situ with a reducing agent to solubilize the sperm DNA. Significantly, centrifugation steps are not required, the process can be easily automated, and can be performed on many samples in parallel. The novel methods and kits are based on filtering selectively solubilized samples through filters that retain sperm by virtue of having uniform pore diameters that: are smaller than sperm, large enough to allow passage of cell debris and solubilized DNA; and are stable to pressure.