Patents by Inventor Alexander Alexeev
Alexander Alexeev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11925934Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.Type: GrantFiled: July 1, 2020Date of Patent: March 12, 2024Assignee: Georgia Tech Research CorporationInventors: Todd Sulchek, Alexander Alexeev, Gonghao Wang
-
METHODS AND SYSTEMS FOR MECHANOPORATION-BASED HIGH-THROUGHPUT PAYLOAD DELIVERY INTO BIOLOGICAL CELLS
Publication number: 20230416781Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: ApplicationFiled: August 25, 2023Publication date: December 28, 2023Applicant: CellFe, Inc.Inventors: Sewoon HAN, Ian SICHER, Alexander ALEXEEV, Ockchul KIM -
Methods and systems for mechanoporation-based high-throughput payload delivery into biological cells
Patent number: 11788050Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: GrantFiled: January 31, 2023Date of Patent: October 17, 2023Assignee: CellFe, Inc.Inventors: Sewoon Han, Ian Sicher, Alexander Alexeev, Ockchul Kim -
METHODS AND SYSTEMS FOR MECHANOPORATION-BASED HIGH-THROUGHPUT PAYLOAD DELIVERY INTO BIOLOGICAL CELLS
Publication number: 20230174918Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: ApplicationFiled: January 31, 2023Publication date: June 8, 2023Applicant: CellFe, Inc.Inventors: Sewoon HAN, Ian SICHER, Alexander ALEXEEV, Ockchul KIM -
Patent number: 11668638Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.Type: GrantFiled: February 4, 2022Date of Patent: June 6, 2023Assignee: Georgia Tech Research CorporationInventors: Todd Sulchek, Alexander Alexeev, Bushra Tasadduq
-
Publication number: 20220298461Abstract: The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.Type: ApplicationFiled: June 11, 2020Publication date: September 22, 2022Applicant: CELLFE, INC.Inventors: Alla ZAMARAYEVA, Alexander ALEXEEV, Todd SULCHEK, Sewoon HAN, Miguel CALERO-GARCIA, Ian SICHER
-
Publication number: 20220228965Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.Type: ApplicationFiled: February 4, 2022Publication date: July 21, 2022Inventors: Todd Sulchek, Alexander Alexeev, Bushra Tasadduq
-
Publication number: 20220213422Abstract: The present disclosure provides methods and systems for cell processing, including delivery of substances into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more substances such as therapeutic or gene-editing reagents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems.Type: ApplicationFiled: May 15, 2020Publication date: July 7, 2022Applicant: CELLFE, INC.Inventors: Alla ZAMARAYEVA, Alexander ALEXEEV, Todd SULCHEK, Miguel CALERO-GARCIA, Ian SICHER, Jocelyn LOO, Sewoon HAN
-
Publication number: 20220204908Abstract: Described herein are methods and systems for cell processing or, more specifically, for introducing various payloads into cells. These methods and systems use a mechanoporation approach in which cells are rapidly compressed and then released to relax while absorbing the payload. More specifically, these methods and systems enable high-throughput mechanoporation with various clogging mitigation features. A cell processing apparatus comprises a shell with an inner shell cylindrical surface, a core with an outer core cylindrical surface, and ridges, supported on and protruding away from one of the inner shell cylindrical surface and the outer core cylindrical surface. The core is disposed inside the shell. The outer core cylindrical surface is concentric with the inner shell cylindrical surface. Each of the ridges forms a ridge gap with the other one of the inner shell cylindrical surface and the outer core cylindrical surface.Type: ApplicationFiled: December 24, 2021Publication date: June 30, 2022Inventors: Sewoon HAN, Alexander ALEXEEV, Todd SULCHEK
-
Patent number: 11268892Abstract: Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.Type: GrantFiled: November 8, 2017Date of Patent: March 8, 2022Assignee: Georgia Tech Research CorporationInventors: Todd Sulchek, Alexander Alexeev, Bushra Tasadduq
-
Publication number: 20220062904Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter, and a plurality of relaxation spaces disposed between the compressive surfaces, flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume Vloss, and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume Vgain and absorb a portion of the plurality of molecules.Type: ApplicationFiled: November 11, 2021Publication date: March 3, 2022Inventors: Todd Sulchek, Alexander Alexeev, Anna Liu
-
Publication number: 20220056393Abstract: The present disclosure provides systems and methods for intracellular delivery. The systems and methods may comprise the use of a cell processing apparatus which may comprise a plurality of compression elements such as ridges. The intracellular delivery may be caused by rapid compression of cells, which may result in a reduction of a cell volume. The compression may occur while the cells pass through gaps formed by at least a subset of the ridges. The cell processing apparatus may further comprise one or more recovery spaces which are positioned between adjacent ridges. The cells may recover at least a portion of the reduced cell volume by absorbing media and/or reagents surrounding the cells while flowing through the recovery spaces. The ridges may also divert less compressible cells into a diversion channel, thereby sorting the cells based on various cell properties and/or preventing clogging within the apparatus.Type: ApplicationFiled: November 8, 2021Publication date: February 24, 2022Applicant: CELLFE, INC.Inventors: Sewoon HAN, Alexander ALEXEEV, Alla ZAMARAYEVA, Todd SULCHEK
-
Patent number: 11198127Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.Type: GrantFiled: November 8, 2017Date of Patent: December 14, 2021Assignee: Georgia Tech Research CorporationInventors: Todd Sulchek, Alexander Alexeev, Anna Liu
-
Publication number: 20210292700Abstract: The present disclosure provides systems and methods for intracellular delivery. The systems and methods may comprise the use of a cell processing apparatus which may comprise a plurality of compression elements such as ridges. The intracellular delivery may be caused by rapid compression of cells, which may result in a reduction of a cell volume. The compression may occur while the cells pass through gaps formed by at least a subset of the ridges. The cell processing apparatus may further comprise one or more recovery spaces which are positioned between adjacent ridges. The cells may recover at least a portion of the reduced cell volume by absorbing media and/or reagents surrounding the cells while flowing through the recovery spaces. The ridges may also divert less compressible cells into a diversion channel, thereby sorting the cells based on various cell properties and/or preventing clogging within the apparatus.Type: ApplicationFiled: June 4, 2021Publication date: September 23, 2021Applicant: CELLFE, INC.Inventors: Sewoon HAN, Alexander ALEXEEV, Alla ZAMARAYEVA, Todd SULCHEK
-
Publication number: 20200330990Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.Type: ApplicationFiled: July 1, 2020Publication date: October 22, 2020Inventors: Todd Sulchek, Alexander Alexeev, Gonghao Wang
-
Patent number: 10717084Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.Type: GrantFiled: November 9, 2016Date of Patent: July 21, 2020Assignee: Georgia Tech Research CorporationInventors: Todd Sulchek, Alexander Alexeev, Gonghao Wang
-
Publication number: 20190360909Abstract: Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.Type: ApplicationFiled: November 8, 2017Publication date: November 28, 2019Inventors: Todd Sulchek, Alexander Alexeev, Bushra Tasadduq
-
Publication number: 20190262835Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.Type: ApplicationFiled: November 8, 2017Publication date: August 29, 2019Inventors: Todd Sulchek, Alexander Alexeev, Anna Liu
-
Publication number: 20180318837Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.Type: ApplicationFiled: November 9, 2016Publication date: November 8, 2018Inventors: Todd SULCHEK, Alexander ALEXEEV, Gonghao WANG
-
Patent number: 8959485Abstract: Methods and apparatus for security protection domain-based testing. A testing framework enables the same certification tests to be run across different protection domains or operation modes, and on different platforms or devices. The testing framework may, for example, be directed to testing implementations of the Java Platform, Micro Edition (Java ME®) using Connected Device Configuration (CDC) or Connected Limited Device Configuration (CLDC) as the configuration layer and Mobile Information Device Profile (MIDP) as the profile layer. Different Mobile Information Device Profile (MIDP) specifications (e.g., MIDP 2.x and MIDP 3.x specifications) may be supported. The testing framework may be deployed in the context of compatibility testing and technology compatibility kits (TCKs). The testing framework may, for example, be applied in compatibility testing for Java ME® platform technology implementations.Type: GrantFiled: June 25, 2012Date of Patent: February 17, 2015Assignee: Oracle International CorporationInventors: Boris Kvartskhava, Vasily Isaenko, Alexander Alexeev