Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: The invention relates to DNA-adapter-molecules for the preparation of DNA-libraries and methods for producing them and their use. The invention is useful for the application in molecular biology, in particular for Next Generation Sequencing and/or Library Multiplexing. The present invention discloses DNA-adapter-molecules, comprising a double-stranded polynucleotide molecule, whereat the 5? end of the first strand is modified in a way, that no binding site for kinases is available, the 3? end of the first strand is modified in a way that no ligation can occur, the 5? end of the reverse strand is modified in a way, that no binding site for a kinase is available, and the 3? end of the reverse strand features a free hydroxyl group (at the 3? position of the last nucleotide).

Abstract: The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5? region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs.

Abstract: The invention relates to a method for detecting a nucleic acid of an organism in a composition, comprising the steps of, (i) amplifying the nucleic acid to be detected, (ii) during or after amplification, hybridizing to said nucleic acid to be detected a first probe that comprises an abasic site additionally optionally carrying a detectable label, (iii) wherein the position of the abasic site corresponds to a position in said nucleic acid to be detected, known to have a polymorphism in said organism, and wherein said nucleic acid is detected if hybridization occurs.

Abstract: The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5? region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs.

Abstract: The invention provides a process for efficient and reliable preparation of organic peroxides, preferably dialkyl peroxides, peroxycarboxylic acids, peroxycarboxylic esters, diacyl peroxides, peroxycarbonate esters, peroxydicarbonates, ketone peroxides and perketals with the aid of at least one static micromixer and an apparatus for performing the process.

Abstract: The invention provides a continuous-flow process for producing a polymeric material. The process of the invention comprises the steps of: introducing at least two solutions, each comprising one or more reactants, into a microreactor to mix the two solutions to form a reacting solution, wherein the one or more reactants are selected from the group consisting of a monomer, an initiator, a coupling agent, a prepolymer, a macromer, and mixtures thereof; feeding continuously the reacting solution into a flow-through polymerization reactor in a coil form, wherein the flow-through polymerization reactor is capable of providing a residence time sufficient for (co)polymerizing the reactants to form a polymer with a desired polydispersity, wherein the flow-through polymerization reactor is immersed in an ultrasonic bath to minimize or substantially eliminate unwanted high-molecular-weight fractions of the polymer.

Abstract: The invention provides a process for efficient and reliable preparation of organic peroxides, preferably dialkyl peroxides, peroxycarboxylic acids, peroxycarboxylic esters, diacyl peroxides, peroxycarbonate esters, peroxydicarbonates, ketone peroxides and perketals with the aid of at least one static micromixer and an apparatus for performing the process.

Abstract: The invention relates to a method which is used to efficiently mark proteins with the help of a micromixer, in addition to a device which enables said inventive method to be carried out in an economical manner and with additional auxiliary agents.

Abstract: The invention relates to a device for drawing a sample and analyzing body fluids, and to a corresponding method. The device comprises a handle (1), which accommodates a pneumatic drive device, and a sensor unit (2) that contains a microneedle (3) on an elastic membrane (4) and at least one sensor system (5). The handle (1) and the sensor unit are detachably connected to one another, and the pneumatic drive device interacts with the sensor unit (2) in such a manner that the drive device exerts pressure onto the elastic membrane (4) so that the microneedle (3) pierces the surface of the skin in order to draw body fluid. During the ensuing decreasing pressure, the elastic membrane (4) returns to its original shape, and body fluid is suctioned, due to the resulting underpressure, from the location of piercing and into the interior of the sensor unit (2) to the at least one sensor system (5).

Abstract: The invention provides a continuous-flow process for producing a polymeric material. The process of the invention comprises the steps of: introducing at least two solutions, each comprising one or more reactants, into a microreactor to mix the two solutions to form a reacting solution, wherein the one or more reactants are selected from the group consisting of a monomer, an initiator, a coupling agent, a prepolymer, a macromer, and mixtures thereof; feeding continuously the reacting solution into a flow-through polymerization reactor in a coil form, wherein the flow-through polymerization reactor is capable of providing a residence time sufficient for (co)polymerizing the reactants to form a polymer with a desired polydispersity, wherein the flow-through polymerization reactor is immersed in an ultrasonic bath to minimize or substantially eliminate unwanted high-molecular-weight fractions of the polymer.