Abstract: The present invention provides for systems and methods for automated quantification of individual compounds in a mixture. The invention is generally concerned with quantitative analysis of solid, liquid, or gas samples and relies on a separation instrument and on a mass detector. The separation instrument provide means to separate the individual compounds typically according to certain physicochemical property, and the mass detector provides means to determine the amount or concentration of an arbitrary compound using an instrument-specific calibration curve. The present invention provides automated capability to analyze such mixtures without user intervention by varying pre-determined control parameters on one or more of the instruments so as to achieve a high degree of analytical resolution of some or all of the individual compounds in the mixture.

Abstract: A method of screening solution conditions suitable for the crystallization of macromolecules with a picogram to a microgram of protein using picoliter or nanoliter volumes is provided. A preferred method comprises preparing a plurality of recipe solutions in milliliter volumes, aspirating protein and recipe solutions, respectively, from a plurality of wells, and dispensing the protein and recipe solutions into a plurality of wells, covering the combined protein and recipe solutions with oil, and maintaining the solution wells until crystallization or precipitation occur therein.

Abstract: A method of screening solution conditions suitable for the crystallization of macromolecules with a picogram to a microgram of protein using picoliter or nanoliter volumes is provided. A preferred method comprises preparing a plurality of recipe solutions in milliliter volumes, aspirating protein and recipe solutions, respectively, from a plurality of wells, and dispensing the protein and recipe solutions into a plurality of wells, covering the combined protein and recipe solutions with oil, and maintaining the solution wells until crystallization or precipitation occur therein.

Abstract: The present invention provides for systems and methods for automated quantification of individual compounds in a mixture. The invention is generally concerned with quantitative analysis of solid, liquid, or gas samples and relies on a separation instrument and on a mass detector. The separation instrument provide means to separate the individual compounds typically according to certain physicochemical property, and the mass detector provides means to determine the amount or concentration of an arbitrary compound using an instrument-specific calibration curve. The present invention provides automated capability to analyze such mixtures without user intervention by varying pre-determined control parameters on one or more of the instruments so as to achieve a high degree of analytical resolution of some or all of the individual compounds in the mixture.

Abstract: A method of screening solution conditions suitable for the crystallization of macromolecules with a picogram to a microgram of protein using picoliter or nanoliter volumes is provided. A preferred method comprises preparing a plurality of recipe solutions in milliliter volumes, aspirating protein and recipe solutions, respectively, from a plurality of wells, and dispensing the protein and recipe solutions into a plurality of wells, covering the combined protein and recipe solutions with oil, and maintaining the solution wells until crystallization or precipitation occur therein.