Patents by Inventor Alexander I. Kuklin

Alexander I. Kuklin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method and system for RNA analysis by matched ion polynucleotide chromatography
    Patent number: 6576133
    Abstract: A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a polymeric separation medium having non-polar surfaces and eluting the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The preferred surfaces are characterized by being substantially free from multivalent cations which are free to interfere with RNA segregation. The elution is preferably performed at a temperature sufficient to denature the RNA. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm. Examples of separation media include beads and monolithic columns.
    Type: Grant
    Filed: January 2, 2001
    Date of Patent: June 10, 2003
    Assignee: Transgenomic, INC
    Inventors: Douglas T. Gjerde, David P. Hornby, Christopher P. Hanna, Alexander I. Kuklin, Robert M. Haefele, Paul D. Taylor
  • Method and system for RNA analysis by matched ion polynucleotide chromatography
    Patent number: 6475388
    Abstract: A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a reverse phase column containing polymeric beads and eluting, preferably under denaturing conditions, the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm.
    Type: Grant
    Filed: April 21, 2000
    Date of Patent: November 5, 2002
    Assignee: Transgenomic, Inc.
    Inventors: Douglas T. Gjerde, David P. Hornby, Christopher P. Hanna, Alexander I. Kuklin
  • Method and system for RNA analysis by matched ion polynucleotide chromatography
    Publication number: 20020153312
    Abstract: A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a reverse phase column containing polymeric beads and eluting, preferably under denaturing conditions, the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm.
    Type: Application
    Filed: April 18, 2002
    Publication date: October 24, 2002
    Inventors: Douglas T. Gjerde, David P. Hornby, Christopher T. Hanna, Alexander I. Kuklin
  • Method and system for RNA analysis by matched ion polynucleotide chromatography
    Publication number: 20010042714
    Abstract: A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a polymeric separation medium having non-polar surfaces and eluting the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The preferred surfaces are characterized by being substantially free from multivalent cations which are free to interfere with RNA segregation. The elution is preferably performed at a temperature sufficient to denature the RNA. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm. Examples of separation media include beads and monolithic columns.
    Type: Application
    Filed: January 2, 2001
    Publication date: November 22, 2001
    Inventors: Douglas T. Gjerde, David P. Hornby, Christopher P. Hanna, Alexander I. Kuklin, Robert M. Haefele, Paul D. Taylor