Patents by Inventor Alexander N. Glazer
Alexander N. Glazer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5906723Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.Type: GrantFiled: August 26, 1996Date of Patent: May 25, 1999Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Alexander N. Glazer, Kaiqin Lao, Adam T. Woolley
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Patent number: 5853992Abstract: Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.Type: GrantFiled: October 4, 1996Date of Patent: December 29, 1998Assignee: The Regents of the University of CaliforniaInventors: Alexander N. Glazer, Richard A. Mathies, Su-Chun Hung, Jingyue Ju
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Patent number: 5783687Abstract: Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.Type: GrantFiled: September 5, 1996Date of Patent: July 21, 1998Assignee: The Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson
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Patent number: 5763162Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.Type: GrantFiled: December 2, 1993Date of Patent: June 9, 1998Assignee: The Regents of University of CaliforniaInventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
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Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
Patent number: 5760201Abstract: Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.Type: GrantFiled: July 12, 1995Date of Patent: June 2, 1998Assignee: The Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson -
Patent number: 5728528Abstract: Fluorescent labels are provided employing energy absorber/donor components and energy acceptor/fluorescer components joined together by a spacer which comprises sugar phosphate monomeric units, particularly ribosyl phosphate monomeric units, where the sugar groups are free of active hydroxyl groups. Particularly, an energy transfer component is substituted at the 5' position of the spacer chain, while the other energy transfer component is substituted at the 1' position of the 3' terminal ribosyl group of the label forming an ET cassette for linking to a nucleic acid sequence with any compositions. By employing combinations of ET components, with a common energy absorber/donor and different fluorescers, one can provide for families of labels which can be tagged to any target molecules and which can be excited at a single wavelength and fluoresce at different wavelengths with large Stokes shifts. The compositions find particular application in sequencing.Type: GrantFiled: September 20, 1995Date of Patent: March 17, 1998Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Alexander N. Glazer, Jingyue Ju
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Patent number: 5646264Abstract: Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.Type: GrantFiled: January 23, 1995Date of Patent: July 8, 1997Assignee: The Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson
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Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
Patent number: 5565554Abstract: Novel fluorescent DNA-staining dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts.Type: GrantFiled: July 29, 1994Date of Patent: October 15, 1996Assignee: The Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson -
Patent number: 5521065Abstract: A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments is disclosed. The method involves reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions. Following hybridization, any of the first and second probes hybridized to contiguous first and second target regions are ligated, and the sample is tested for the presence of expected probe ligation product. The presence of ligated product indicates that the target sequence is present in the sample.Type: GrantFiled: June 8, 1994Date of Patent: May 28, 1996Assignee: Applied Biosystems, Inc.Inventors: Norman M. Whiteley, Michael W. Hunkapiller, Alexander N. Glazer
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Patent number: 5401847Abstract: Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.Type: GrantFiled: January 27, 1993Date of Patent: March 28, 1995Assignee: Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson
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Patent number: 5312921Abstract: Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.Type: GrantFiled: May 14, 1993Date of Patent: May 17, 1994Assignee: Regents of the University of CaliforniaInventors: Alexander N. Glazer, Scott C. Benson
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Patent number: 5242794Abstract: The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed.Type: GrantFiled: June 5, 1989Date of Patent: September 7, 1993Assignee: Applied Biosystems, Inc.Inventors: Norman M. Whiteley, Michael W. Hunkapiller, Alexander N. Glazer
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Patent number: 5055556Abstract: Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.Type: GrantFiled: October 25, 1985Date of Patent: October 8, 1991Assignee: The Board of Trustees of the Leland Stanford Jr. Univ.Inventors: Lubert Stryer, Alexander N. Glazer, Vernon T. Oi
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Patent number: 4883750Abstract: The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed.Type: GrantFiled: December 13, 1984Date of Patent: November 28, 1989Assignee: Applied Biosystems, Inc.Inventors: Norman M. Whiteley, Michael W. Hunkapiller, Alexander N. Glazer
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Patent number: 4859582Abstract: Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.Type: GrantFiled: February 13, 1985Date of Patent: August 22, 1989Assignee: The Board of Trustees of the Leland Stanford Jr. UniversityInventors: Lubert Stryer, Alexander N. Glazer, Vernon T. Oi
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Patent number: 4857474Abstract: This invention provides a class of phycoerythrins useful in diagnostic and detection protocols wherein a fluorescent label is required. The unique spectral properties of the phycoerythrins described herein provide for increased sensitivity and alternative uses in assays employing them.Type: GrantFiled: March 29, 1985Date of Patent: August 15, 1989Assignee: Research CorporationInventors: John B. Waterbury, Stanley W. Watson, Alexander N. Glazer, Linda J. Ong
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Patent number: 4542104Abstract: Fluorescent conjugates are employed providing combinations of a fluorescent sensitizer and a fluorescent phycobiliprotein. The conjugates find use in applications where large Stokes shifts, high absorption coefficients and high fluorescence quantum yields are desired. Particularly, combinations of phycobiliproteins are employed where the wavelength of excitation may be 50 nm or more different from the emission wavelength.Type: GrantFiled: April 6, 1983Date of Patent: September 17, 1985Assignee: The Board of Trustees of the Leland Stanford Jr. Univ.Inventors: Lubert Stryer, Alexander N. Glazer
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Patent number: 4520110Abstract: Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.Type: GrantFiled: December 30, 1982Date of Patent: May 28, 1985Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Lubert Stryer, Alexander N. Glazer, Vernon T. Oi