Patents by Inventor Alexander Nierth

Alexander Nierth has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR WITH THE USE OF LARGE STOKES SHIFT FLUORESCENT DYES
    Publication number: 20240035077
    Abstract: The present invention allows for the expansion of multiplexing capabilities of common PCR devices by using fluorogenic PCR probes made of large Stokes shift (LSS) fluorescent dyes. With this approach, no changes of the hardware or software components in the instrument are required.
    Type: Application
    Filed: December 21, 2021
    Publication date: February 1, 2024
    Inventors: Alexander Nierth, Fangnian Wang, Jin Wang
  • MODIFIED NUCLEOSIDE PHOSPHATES WITH HIGH THERMAL STABILITY
    Publication number: 20230220464
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: March 16, 2023
    Publication date: July 13, 2023
    Inventor: Alexander Nierth
  • DICATIONIC FLUORESCENT DYES
    Publication number: 20230050863
    Abstract: The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.
    Type: Application
    Filed: December 7, 2020
    Publication date: February 16, 2023
    Inventor: Alexander Nierth
  • Reagents and methods for post-synthetic modification of nucleic acids
    Patent number: 11384376
    Abstract: The present invention relates to compositions and methods (reagents and protocols) for the post-synthetic modification of nucleic acids obtained from solid-phase oligonucleotide synthesis with a label (such as fluorescent dyes). The coupling reagent is the triazine-based salt 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMT-MM) in the presence of a counteranion.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: July 12, 2022
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Alexander Nierth
  • Photocleavable nucleotide reagents with high stability
    Patent number: 11078534
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: August 3, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • Catalysts for reversing formaldehyde adducts and crosslinks
    Patent number: 10995104
    Abstract: Catalysts act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting catalysts to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: May 4, 2021
    Assignee: Roche Molecular System, Inc.
    Inventor: Alexander Nierth
  • REAGENTS AND METHODS FOR POST-SYNTHETIC MODIFICATION OF NUCLEIC ACIDS
    Publication number: 20200017895
    Abstract: The present invention relates to compositions and methods (reagents and protocols) for the post-synthetic modification of nucleic acids obtained from solid-phase oligonucleotide synthesis with a label (such as fluorescent dyes). The coupling reagent is the triazine-based salt 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMT-MM) in the presence of a counteranion.
    Type: Application
    Filed: May 28, 2019
    Publication date: January 16, 2020
    Inventor: Alexander Nierth
  • MODIFIED NUCLEOSIDE PHOSPHATES WITH HIGH THERMAL STABILITY
    Publication number: 20190292596
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: March 19, 2019
    Publication date: September 26, 2019
    Inventor: Alexander Nierth
  • PHOTOCLEAVABLE NUCLEOTIDE REAGENTS WITH HIGH STABILITY
    Publication number: 20190161797
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: November 26, 2018
    Publication date: May 30, 2019
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • Catalysts for reversing formaldehyde adducts and crosslinks
    Publication number: 20180370992
    Abstract: Catalysts act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting catalysts to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.
    Type: Application
    Filed: May 29, 2018
    Publication date: December 27, 2018
    Inventor: Alexander Nierth