Abstract: The present invention relates to a method for cultivation and manufacturing efficiently algae containing useful substances in cells thereof in a high concentration. According to conventional cultivation apparatus, shear stress applied to algae causes difficulty of high density cultivation; however, by cultivation of algae by using a convenient cultivation apparatus having a dome shape, conical shape, or cylindrical shape and made by a clear material, high density cultivation has been realized without shear stress. The present cultivation apparatus realizes more efficient cultivation in combined use with a separate gas discharging apparatus. In addition, the present invention can be applied to a wide variety of algal species having ability to produce highly unsaturated fatty acid, a photosynthetic pigment, or a polysaccharide.

Abstract: A device for the manipulation of a biological material by a magnetic field is presented. The device comprises a magnetic field source coupled to a current source. The current source is of a kind supplying an electric current of at least two electrical degree shifted phases. The magnetic field source comprises a two-part inductor, each inductor part producing a coordinate varying magnetic field (CVMF). Each inductor part is formed by at least two conductors aligned in a spaced-apart relationship, wherein each of the at least two conductors is connectable to a different phase of the current source, and has two spaced-apart parts arranged such that when the conductor is connected to the current source, the electric current flows in its two parts in opposite directions, respectively. The conductors of each inductor part are arranged such that each two locally adjacent conductor parts are associated with two different phases of the electric current source.

Abstract: A device for the manipulation of a biological material by a magnetic field is presented. The device comprises a magnetic field source coupled to a current source. The current source is of a kind supplying an electric current of at least two electrical degree shifted phases. The magnetic field source comprises a two-part inductor, each inductor part producing a coordinate varying magnetic field (CVMF). Each inductor part is formed by at least two conductors aligned in a spaced-apart relationship, wherein each of the at least two conductors is connectable to a different phase of the current source, and has two spaced-apart parts arranged such that when the conductor is connected to the current source, the electric current flows in its two parts in opposite directions, respectively. The conductors of each inductor part are arranged such that each two locally adjacent conductor parts are associated with two different phases of the electric current source.

Abstract: This invention relates to a culture device of a domed shape, a conical shape, or a cylindrical shape of a closed type used for culture of microalgae, and a gas discharge device set so as to be movable in the culture device. The culture device is basically composed of a transparent inside member (a semispherical dome, a conical peripheral wall, or a cylindrical peripheral wall), a transparent outside member, and a bottom portion connecting the lower ends of the two members, a cylindrical opening portion is provided at the top part of the outside member, and a gas introducing member and a discharging member of a culture solution are provided in the bottom portion. The gas discharge device is basically composed of two opposed rectangular base plates, a bubble guide member, and a discharge nozzle. The culture solution can be agitated without mechanical agitation and the culture can be carried out in high concentrations.