Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image and/or printing a three-dimensional structures, the system comprising: a medium comprising an acid-sensitive photoinitiator, a photoacid, monomers, donors, and acceptors, wherein the acceptor has a non-fluorescent state and a fluorescent state, wherein at one wavelength of optical excitation an optical molecular switch molecule has a first state, and at a second state the optical molecular switch molecule fluoresces at a second wavelength of excitation; and at least a first light source and a second light source into the medium, wherein light emitted by the at least first and second light sources are directed to contact the acid-sensitive photoinitiator, wherein a first wavelength activates the photo acid, and the second wavelength triggers polymerization of the monomers.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image and/or printing a three-dimensional structures, the system comprising: a medium comprising an acid-sensitive photoinitiator, a photoacid, monomers, donors, and acceptors, wherein the acceptor has a non-fluorescent state and a fluorescent state, wherein at one wavelength of optical excitation an optical molecular switch molecule has a first state, and at a second state the optical molecular switch molecule fluoresces at a second wavelength of excitation; and at least a first light source and a second light source into the medium, wherein light emitted by the at least first and second light sources are directed to contact the acid-sensitive photoinitiator, wherein a first wavelength activates the photo acid, and the second wavelength triggers polymerization of the monomers.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image and/or printing a three dimensional structures, the system comprising: a medium comprising a photocurable monomer and an optical molecular switch molecule that has a non-fluorescent state and a fluorescent state, wherein the monomer forms a polymer upon exposure to the emitted light. Also taught are novel fluorescent photoswitch molecules.

Abstract: The present invention provides a method for the rapid monitoring of biological analytes in a point-of-care setting by providing a smart phone; providing a sample chamber; providing a sample; providing a dark box with a smartphone holder attached to the dark box top with the camera opening positioned about the aperture, adding a biological specimen suspected of containing a biological analyte in the sample chamber; adding a bis(2,4,6-trichlorophenyl) oxalate, an imidazole and a fluorophore to the sample chamber to react with the biological analyte; placing the sample chamber into the dark box; generating an emission from the fluorophore in response to the reaction with the biological analyte; and recording a set of time-lapse images of the emission with the smartphone.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image and/or printing a three dimensional structures, the system comprising: a medium comprising a photocurable monomer and an optical molecular switch molecule that has a non-fluorescent state and a fluorescent state, wherein the monomer forms a polymer upon exposure to the emitted light. Also taught are novel fluorescent photoswitch molecules.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image and/or printing a three dimensional structures, the system comprising: a medium comprising a photocurable monomer and an optical molecular switch molecule that has a non-fluorescent state and a fluorescent state, wherein the monomer forms a polymer upon exposure to the emitted light. Also taught are novel fluorescent photoswitch molecules.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image, the system comprising: a medium comprising a optical molecular switch molecule, wherein the optical molecular switch molecule has a non-fluorescent state and a fluorescent state, wherein at one wavelength of optical excitation the molecule has a first state, and at a second state the molecule fluoresces a second wavelength of excitation; and at least a first light source and a second light source into the medium, wherein light emitted by the at least first and second light sources are directed to contact the optical molecular switch molecule, e.g., scanning, weaving, diagonally or other pattern; wherein the optical molecular switch molecule is converted into a fluorescent “on state” by irradiation from the first light source, and the second light causes the optical molecular switch to emit light.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present disclosure provides compositions for in vivo imaging of hydrogen peroxide; and methods for detecting hydrogen peroxide in vivo. The compositions and methods find use in various diagnostic applications, which are also provided.

Abstract: The present disclosure provides a method of treating a subject that is resistant to one or more drugs by identifying a subject having one or more drug resistant cells; administering to the subject a pharmaceutically effective amount of an inhibitor compound, and contacting one or more drug resistant cells with the inhibitor compound to reduce the export of the inhibitor compound from the one or more drug resistant tumor cells and to block the transport of drug(s) from the one or more drug resistant cells.

Abstract: The present invention provides a method for the rapid monitoring of biological analytes in a point-of-care setting by providing a smart phone; providing a sample chamber; providing a sample; providing a dark box with a smartphone holder attached to the dark box top with the camera opening positioned about the aperture, adding a biological specimen suspected of containing a biological analyte in the sample chamber; adding a bis(2,4,6-trichlorophenyl) oxalate, an imidazole and a fluorophore to the sample chamber to react with the biological analyte; placing the sample chamber into the dark box; generating an emission from the fluorophore in response to the reaction with the biological analyte; and recording a set of time-lapse images of the emission with the smartphone.

Abstract: The present invention provides a method for the rapid monitoring of biological analytes in a point-of-care setting by providing a smart phone; providing a sample chamber; providing a sample; providing a dark box with a smartphone holder attached to the dark box top with the camera opening positioned about the aperture, adding a biological specimen suspected of containing a biological analyte in the sample chamber; adding a bis(2,4,6-trichlorophenyl) oxalate, an imidazole and a fluorophore to the sample chamber to react with the biological analyte; placing the sample chamber into the dark box; generating an emission from the fluorophore in response to the reaction with the biological analyte; and recording a set of time-lapse images of the emission with the smartphone.

Abstract: The present disclosure provides compositions for in vivo imaging of hydrogen peroxide; and methods for detecting hydrogen peroxide in vivo. The compositions and methods find use in various diagnostic applications, which are also provided.

Abstract: The present invention includes a system, apparatus and method for generating a three-dimensional image, the system comprising: a medium comprising a optical molecular switch molecule, wherein the optical molecular switch molecule has a non-fluorescent state and a fluorescent state, wherein at one wavelength of optical excitation the molecule has a first state, and at a second state the molecule fluoresces a second wavelength of excitation; and at least a first light source and a second light source into the medium, wherein light emitted by the at least first and second light sources are directed to contact the optical molecular switch molecule, e.g., scanning, weaving, diagonally or other pattern; wherein the optical molecular switch molecule is converted into a fluorescent “on state” by irradiation from the first light source, and the second light causes the optical molecular switch to emit light.

Abstract: The present invention provides a method for the rapid monitoring of biological analytes in a point-of-care setting by providing a smart phone; providing a sample chamber; providing a sample; providing a dark box with a smartphone holder attached to the dark box top with the camera opening positioned about the aperture, adding a biological specimen suspected of containing a biological analyte in the sample chamber; adding a bis(2,4,6-trichlorophenyl) oxalate, an imidazole and a fluorophore to the sample chamber to react with the biological analyte; placing the sample chamber into the dark box; generating an emission from the fluorophore in response to the reaction with the biological analyte; and recording a set of time-lapse images of the emission with the smartphone.

Abstract: The invention provides reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells.