Patents by Inventor Alexei Vorobiev

Alexei Vorobiev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
    Patent number: 9085800
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Grant
    Filed: March 23, 2012
    Date of Patent: July 21, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • Minor groove binder phosphoramidites and methods of use
    Patent number: 9056887
    Abstract: Minor groove binder phosphoramidites having the formula M-L-PA, wherein M is a minor groove binder comprising a protected heteroaromatic amine, L is a linker, and PA is a phosphoramidite group, have been synthesized. Preferred methods of synthesis include synthesizing a minor groove binder intermediate containing a transiently protected hydroxyl group, protecting heteroaromatic amines of the corresponding minor groove binder intermediate as carbamate intermediates, reacting the carbamate intermediate to remove the transient protecting group to yield carbamate-protected minor groove binder agent as an intermediate with a free hydroxyl group, and converting the intermediate with a free hydroxyl group to the desired minor groove binder phosphoramidite. These minor groove binder phosphoramidites are useful in the preparation of oligonucleotide conjugates, particularly those for use as probes and primers.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: June 16, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov
  • Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
    Patent number: 8969003
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogs can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogs can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: March 3, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
    Publication number: 20130261014
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 3, 2013
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • Phosphonate fluorescent dyes and conjugates
    Patent number: 8389745
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 29, 2011
    Date of Patent: March 5, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • MINOR GROOVE BINDER PHOSPHORAMIDITES AND METHODS OF USE
    Publication number: 20130030166
    Abstract: Minor groove binder phosphoramidites having unique structures have been synthesized according to particular methods. These minor groove binder phosphoramidites are useful in the preparation of oligonucleotide conjugates, particularly those for use as probes and primers.
    Type: Application
    Filed: July 24, 2012
    Publication date: January 31, 2013
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov
  • FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
    Publication number: 20120244535
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Application
    Filed: March 23, 2012
    Publication date: September 27, 2012
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • PHOSPHONATE FLUORESCENT DYES AND CONJUGATES
    Publication number: 20120116079
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: August 29, 2011
    Publication date: May 10, 2012
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Phosphonate fluorescent dyes and conjugates
    Patent number: 8008522
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: January 6, 2010
    Date of Patent: August 30, 2011
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • PHOSPHONATE FLUORESCENT DYES AND CONJUGATES
    Publication number: 20100261903
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: January 6, 2010
    Publication date: October 14, 2010
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Phosphonylated fluorescent dyes and conjugates
    Patent number: 7767834
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: August 3, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7718374
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: May 18, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Phosphonate fluorescent dyes and conjugates
    Patent number: 7671218
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: March 2, 2010
    Assignee: Elitech Holdings B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF HIGHLY POLYMORPHIC TARGET SEQUENCES
    Publication number: 20090087922
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Application
    Filed: January 28, 2008
    Publication date: April 2, 2009
    Applicant: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • PHOSPHONYLATED FLUORESCENT DYES AND CONJUGATES
    Publication number: 20080207887
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: November 8, 2007
    Publication date: August 28, 2008
    Applicant: EPOCH BIOSCIENCES, INC.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7348146
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: March 25, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Phosphonate fluorescent dyes and conjugates
    Publication number: 20070172832
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: August 12, 2005
    Publication date: July 26, 2007
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Compounds and methods for fluorescent labeling
    Publication number: 20060204990
    Abstract: Compounds useful in labeling biological materials are provided along with methods for their use.
    Type: Application
    Filed: February 17, 2006
    Publication date: September 14, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev, Michael Reed, Nicolaas Vermeulen
  • Phosphonylated fluorescent dyes and conjugates
    Publication number: 20060199955
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: February 21, 2006
    Publication date: September 7, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Compounds and methods for fluorescent labeling
    Publication number: 20050277144
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Application
    Filed: May 10, 2005
    Publication date: December 15, 2005
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev, Michael Reed, Nicolaas Vermeulen