Patents by Inventor Alexey Terskikh
Alexey Terskikh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9834584Abstract: Nucleic acid compositions encoding chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.Type: GrantFiled: November 30, 2006Date of Patent: December 5, 2017Assignee: Takara Bio USA, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 9598670Abstract: We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: GrantFiled: November 26, 2014Date of Patent: March 21, 2017Assignee: BURNHAM INSTITUTE FOR MEDICAL RESEARCHInventors: Alexey Terskikh, Ruchi Bajpai
-
Patent number: 9157061Abstract: We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: GrantFiled: June 20, 2011Date of Patent: October 13, 2015Assignee: BURNHAM INSTITUTE FOR MEDICAL RESEARCHInventors: Alexey Terskikh, Ruchi Bajpai
-
Publication number: 20150152380Abstract: We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: ApplicationFiled: November 26, 2014Publication date: June 4, 2015Inventors: Alexey Terskikh, Ruchi Bajpai
-
Publication number: 20120034618Abstract: We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: ApplicationFiled: June 20, 2011Publication date: February 9, 2012Applicant: SANFORD-BURNHAM MEDICAL RESEARCH INSTITUTEInventors: Alexey Terskikh, Ruchi Bajpai
-
Patent number: 8012682Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: September 6, 2011Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7964402Abstract: We used Accutase™, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: GrantFiled: May 24, 2007Date of Patent: June 21, 2011Assignee: Sanford-Burnham Medical Research InstituteInventors: Alexey Terskikh, Ruchi Bajpai
-
Publication number: 20110091568Abstract: Compositions for application to the skin of individuals in need thereof are provided that include media conditioned by the growth of human stem cells, particularly human neural stem cells. In some embodiments, the stem cells are grown without the use of feeder layers.Type: ApplicationFiled: April 7, 2010Publication date: April 21, 2011Inventors: Stuart A. Lipton, Alexey Terskikh
-
Publication number: 20100105876Abstract: The present invention concerns an oligomer comprising more than 2 units, wherein each unit comprises a peptidic domain capable of oligomerizing and a domain capable of binding to an acceptor (ligand), wherein the oligomerizing domain is not an antibody or a functional antibody fragment from the constant region. Also described is the use and synthesis of this oligomer.Type: ApplicationFiled: April 25, 2002Publication date: April 29, 2010Inventors: Alexey Terskikh, Reto Crameri, Jean-Pierre Mach, Jean-Marc Le Doussal, Andrey Kajava
-
Patent number: 7537915Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red”(NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green”(NFP-8), and Discosoma sp.“magenta”(NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: May 26, 2009Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7442521Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific protiens of interest include protiens obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp. “green” (NFP-8), and Discosoma sp. “magneta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: October 28, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7442522Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4),Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: October 28, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Publication number: 20080254004Abstract: This invention provides pure populations of neural precursor cells, capable of differentiation into neurons, glial cells, and astrocytes. The populations are obtained by culturing stem cell populations (such as embryonic stem cells) in a cocktail of growth conditions that initiates differentiation, and establishes the neural precursor population. The precursors can be further differentiated in culture into a variety of different neural phenotypes. The neural precursors can be generated in pure form (at least 99%) and in large quantities for use in drug screening and the treatment of neurological disorders.Type: ApplicationFiled: February 9, 2006Publication date: October 16, 2008Applicant: Burnham Institute For Medical ResearchInventors: Alexey Terskikh, Ruchi Bajpai
-
Patent number: 7344862Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2),Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: March 18, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Publication number: 20080058221Abstract: We used Accutase™, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.Type: ApplicationFiled: May 24, 2007Publication date: March 6, 2008Inventors: Alexey Terskikh, Ruchi Bajpai
-
Patent number: 7338784Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red”(NFP-6), Anemonia sulcata (NFP-7), Discosoma sp. “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: March 4, 2008Assignee: Clontech Laboratories, IncInventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7338783Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red”(NFP-6), Anemonia sulcata (NFP-7), Discosoma sp. “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: March 4, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7338782Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: March 4, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Patent number: 7338785Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp. “magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: GrantFiled: November 30, 2006Date of Patent: March 4, 2008Assignee: Clontech Laboratories, Inc.Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh
-
Publication number: 20080022419Abstract: Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the proteins encoded by the same, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp. “magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins.Type: ApplicationFiled: November 30, 2006Publication date: January 24, 2008Inventors: Sergey Lukyanov, Arcady Fradkov, Yulil Labas, Mikhail Matz, Alexey Terskikh