Patents by Inventor Alicia DiCicco

Alicia DiCicco has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • T4 DNA Ligase Variants with Increased Resistance to Salt
    Publication number: 20240067951
    Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
    Type: Application
    Filed: May 10, 2023
    Publication date: February 29, 2024
    Inventors: Alicia DiCicco, Zhenyu Zhu, Aine Quimby
  • T4 DNA ligase variants with increased thermostability
    Patent number: 11788082
    Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity at temperatures above 37° C. compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. T4 Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
    Type: Grant
    Filed: February 22, 2022
    Date of Patent: October 17, 2023
    Assignee: ABclonal Science, Inc.
    Inventors: Zhenyu Zhu, Aine Quimby, Dapeng Sun, Alicia DiCicco
  • T4 DNA Ligase Variants with Increased Ligation Efficiency
    Publication number: 20230295707
    Abstract: The invention includes a mutant T4 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T4 DNA ligase. The mutant T4 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, as described more fully in the Summary.
    Type: Application
    Filed: March 21, 2022
    Publication date: September 21, 2023
    Inventors: Zhenyu Zhu, Aine Quimby, Dapeng Sun, Alicia DiCicco
  • T4 DNA Ligase Variants with Increased Thermostability
    Publication number: 20230265412
    Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity at temperatures above 37° C. compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. T4 Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
    Type: Application
    Filed: February 22, 2022
    Publication date: August 24, 2023
    Inventors: Zhenyu Zhu, Aine Quimby, Dapeng Sun, Alicia DiCicco
  • T4 DNA Ligase Variants with Increased Ligation Efficiency
    Publication number: 20230212550
    Abstract: The invention includes a mutant T4 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T4 DNA ligase. The mutant T4 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, as described more fully in the Summary with reference to the Sequence Listing.
    Type: Application
    Filed: December 2, 2022
    Publication date: July 6, 2023
    Inventors: Zhenyu Zhu, Aine Quimby, Dapeng Sun, Alicia DiCicco
  • T4 DNA ligase variants with increased resistance to salt
    Patent number: 11685913
    Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
    Type: Grant
    Filed: February 18, 2022
    Date of Patent: June 27, 2023
    Assignee: AbClonal Science, Inc.
    Inventors: Alicia DiCicco, Zhenyu Zhu, Aine Quimby