Abstract: To provide a method for producing transferrin by secretion with high productivity using a transformant of Schizosaccharomyces pombe, and a transformant suitable for the method. A transformant using Schizosaccharomyces pombe as a host, comprising a transferrin gene and a secretion signal peptide gene expressing transferrin to which a secretion signal peptide which functions in the host binds, located in an upstream region of the transferrin gene, and having gas2 gene endogenous to the host deleted or inactivated; and a method for producing transferrin, which comprises culturing the transformant in a liquid medium and obtaining transferrin from the liquid medium.

Abstract: To provide a method for producing transferrin by secretion with high productivity using a transformant of Schizosaccharomyces pombe, and a transformant suitable for the method. A transformant using Schizosaccharomyces pombe as a host, comprising a transferrin gene and a secretion signal peptide gene expressing transferrin to which a secretion signal peptide which functions in the host binds, located in an upstream region of the transferrin gene, and having gas2 gene endogenous to the host deleted or inactivated; and a method for producing transferrin, which comprises culturing the transformant in a liquid medium and obtaining transferrin from the liquid medium.

Abstract: To provide a resin composition which is used to coat the substrate surface, thereby to selectively trap a variety of target substances via a ligand which specifically binds to the target substances on the substrate surface.

Abstract: A cell culture vessel includes a bottom, a peripheral wall, and a partition. The bottom of the cell culture vessel has a culture surface with a plurality of wells. The peripheral wall extends upwardly from a periphery of the bottom. The partition partitions a region on the culture surface surrounded by the peripheral wall into a plurality of sub-regions.

Abstract: The efficiency of production of a heterologous protein by a transformant of a yeast host is improved. A method of constructing a host for expression of a foreign gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes (especially, metalloprotease genes and serine protease genes) of Schizosaccharomyces pombe, a host in which the above-mentioned gene is deleted or inactivated, a transformant obtained by introducing a foreign gene into the host and a method of producing a heterologous protein using the transformant. The protease-associated gene is at least one gene selected from the group consisting of psp3 (SPAC1006.01), is sxa2 (SPAC1296.03c), ppp51 (SPAC22G7.01c) and ppp52 (SPBC18A7.01) or at least two genes selected from the group consisting of metalloprotease genes and serine protease genes, especially, three genes psp3 (SPAC1006.01), isp6 (SPAC4A8.04) and ppp53 (SPAP14E8.04).

Abstract: To provide a method for transforming S. pombe for creating a transformant with a high stability of maintenance after passage and enables the steady production of a heterologous protein of interest, a transformant produced by the method and a method for producing a heterologous protein using the resultant transformant. A method for transforming Schizosaccharomyces pombe by using a vector carrying an expression cassette (containing a promoter capable of functioning in Schizosaccharomyces pombe, a heterologous protein structural gene and a terminator), and having recombination region(s) at which homologous recombination with each chromosome of Schizosaccharomyces pombe is to be achieved, which comprises integrating the expression cassette into the Tf2 transposon gene position(s) in the chromosome(s) of Schizosaccharomyces pombe by homologous recombination, a transformant created by the method and a method for producing a heterologous protein using the resultant transformant.

Abstract: The efficiency of production of a heterologous protein by a transformant of a yeast host is improved. A method of constructing a host for expression of a foreign gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes (especially, metalloprotease genes and serine protease genes) of Schizosaccharomyces pombe, a host in which the above-mentioned gene is deleted or inactivated, a transformant obtained by introducing a foreign gene into the host and a method of producing a heterologous protein using the transformant. The protease-associated gene is at least one gene selected from the group consisting of psp3 (SPAC1006.01), is sxa2 (SPAC1296.03c), ppp51 (SPAC22G7.01c) and ppp52 (SPBC18A7.01) or at least two genes selected from the group consisting of metalloprotease genes and serine protease genes, especially, three genes psp3 (SPAC1006.01), isp6 (SPAC4A8.04) and ppp53 (SPAP14E8.04).